- Volume 60 Issue 7
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Changes in free amino acid content and hardness of beef while dry-aging with Mucor flavus
- Hanagasaki, Takashi (Okinawa Prefectural Industrial Technology Center) ;
- Asato, Naokazu (Okinawa Prefectural Livestock Research Center)
- Received : 2018.03.29
- Accepted : 2018.06.27
- Published : 2018.07.31
Background: A mold strain thought to be suitable for dry-aging process was isolated. The information about the scientific aspects of molds related to dry-aging beef is scarce. We, therefore, conducted aging trials to determine the characteristics of the isolated mold strain associated with dry-aging process. Specifically, during the dry-aging of beef with the mold strain, the changes in the free amino acid content, hardness, productive loss, drip and cooking loss were analyzed. These characteristics were compared with those obtained while dry-aging in the absence of a mold. Results: The isolated mold strain was identified as Mucor flavus. The free amino acid content in the mold-aging beef decreased or remained constant during the aging process. However, that in the trimming sections of the beef dramatically increased in the presence of mold. In addition, hardness of mold-aging beef gradually decreased during the aging process and finally decreased significantly. Conclusion: Amino acids such as GABA (gamma-aminobutyric acid), proline, and aspartic acid were produced by our mold strain, M. flavus during its growth on beef meat, and the mold conferred savory odors to the dry-aged beef.
Supported by : Science and Technology Promotion Division of Okinawa
- Julie D. Thompson, Desmond G. Higgins, Toby J. Gibson. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 1994;22(22):4673-4680. https://doi.org/10.1093/nar/22.22.4673
- Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol. 1980;16:111-20. https://doi.org/10.1007/BF01731581
- Laster MA. Tenderness, Flavor, and yield assessments of dry aged beef. M.S. Thesis. College Station: Texas AM University; 2007.
- Muramoto T, Maeno K, Okada Y, Tezuka S, Kamata T. Relationship between shear force and tenderness of Japanese shorthorn beef. Tohoku J Anim Sci Technol. 2014;64(1):7-12.
- Neil P. Dry aging beef. Int J Gastoronomy Food Sci. 2012;1:78-80. https://doi.org/10.1016/j.ijgfs.2011.11.005
- Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol. 1987;4:406-25.
- Savell JW. Dry-aging of beef, exective summery. National Cattlemen's beef association. 2008. http://www.beefresearch.org/cmdocs/beefresearch/ dry%20Aging%20of%20beef.pdf.
- Stephanie M-S, Patrice N, Emmanuel C, Jean-Luc J. Mucor: a Janus-faced fungal genus with human health impact and industrial applications. Fungal Biol Rev. 2017;31:12-32. https://doi.org/10.1016/j.fbr.2016.11.002
- Sugioka K, Tasaki T, Sakai H, Ieiri S, Araki T. Study of free amino acid in aging of beef. Proceedings of the annual meeting 2015 Okayama of Japan Society for Bioscience, Biotechnology, and Agrochemistry; 2015. p. 2F26P18.
- Walther G, Pawlowska J, Alastruey-Izquierdo A, Wrzosek M, Rodriguez-Tudela JL, Dolatabadi S, Chakrabarti A, de Hoog GS. DNA barcoding in Mucorales: an inventory of biodiversity. Persoonia. 2013;30:11-47. https://doi.org/10.3767/003158513X665070
- Schipper MAA. On Mucor mucedo, Mucor flavus and related species. Stud Mycol. 1975;10:1-33.
- Warren KE, Kastner CL. A comparison of dry aged and vacuum-aged beef striploins. J Muscle Foods. 1992;3:151-7. https://doi.org/10.1111/j.1745-4573.1992.tb00471.x
- White TJ, Bruns T, Lee S, Taylor J. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR protocols, a guide to methods and applications; 1990. p. 315-22.
- Abdou AM, Higashiguchi S, Horie K, Kim K, Hatta H, Yokogoshi H. Relaxation immunity enhancement effects of gamma-aminobutyric acid (GABA) administration in humans. BioFactors. 2006;26(3):201-8. https://doi.org/10.1002/biof.5520260305
- Baird B. Dry aging enhances palatability of beef, beef safety and quality. 2008. https://www.beefresearch.org/CMDocs/BeefResearch/PE_Issues_ Update/Dry_aging_enhances_palatability_of_beef.pdf. Accessed March April 2008.
- Campbell RE, Hunt MC, Levis P, Chambers Iv E. Dry-aging effects on palatability of beef longissimus muscle. J Food Sci. 2001;66:196-9. https://doi.org/10.1111/j.1365-2621.2001.tb11315.x
- Dashmaa D, Tripathi VK, Cho S, Kim Y, Hwang I. Dry aging of beef; review. J Anim Sci Technol. 2016;58:20. https://doi.org/10.1186/s40781-016-0101-9
- Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution. 1985;39:783-91. https://doi.org/10.1111/j.1558-5646.1985.tb00420.x