Yield, Nutritional Content, and Antioxidant Activity of Pleurotus ostreatus on Corncobs Supplemented with Herb Residues

  • Jin, Zhiqiang (Department of Biological Science and Technology, Changzhi University) ;
  • Li, Yunling (Department of Biological Science and Technology, Changzhi University) ;
  • Ren, Jiahong (Department of Biological Science and Technology, Changzhi University) ;
  • Qin, Nan (Department of Pharmaceutical and Food Engineering, Shanxi University of Traditional Chinese Medicine)
  • Received : 2018.01.29
  • Accepted : 2018.02.13
  • Published : 2018.03.31


Improper disposal of herb residues in China has caused severe problems to the surrounding environment and human safety. Three herb residues, i.e., compound Kushen injection residues (CKI) and part one and part two of Qizhitongluo Capsule residues (QC1 and QC2, respectively), were used for the cultivation of Pleurotus ostreatus. The effect of the supplementation of corncobs (CC) with different herb residues on yield, nutritional composition, and antioxidant activity of P. ostreatus was investigated. Compared to the control, the higher mycelial growth rate was observed on substrates CC +30% CKI and CC +30% QC1, while the higher yield was obtained from substrates CC +30% QC2 and CC +30% CKI. Moreover, chemical analysis of fruit bodies revealed that the addition of herb residues to CC significantly increased proteins, amino acids, ashes, minerals (Na and Ca), and total phenolic contents but significantly reduced carbohydrates and $IC_{50}$ values of DPPH radicals. In addition, no heavy metals (Pb, Cd, and As) were detected in the fruiting bodies harvested from different substrate combinations. These results demonstrated that mixtures of CC with herb residues might be utilized as a novel, practical, and easily available substrate for the cultivation of P. ostreatus, which is beneficial for the effective management of herb residues.


  1. Patel Y, Naraian R, Singh VK. Medicinal properties of Pleurotus species (Oyster mushroom): a review. World J Fungal Plant Biol. 2012;3:1-12.
  2. Sardar H, Ali MA, Anjum MA, et al. Agro-industrial residues influence mineral elements accumulation and nutritional composition of king oyster mushroom (Pleurotus eryngii). Sci Hortic. 2017;225:327-334.
  3. Chanakya HN, Malayil S, Vijayalakshmi C. Cultivation of Pleurotus spp. on a combination of anaerobically digested plant material and various agro-residues. Energy Sustain Dev. 2015;27:84-92.
  4. Koutrotsios G, Mountzouris KC, Chatzipavlidis I, et al. Bioconversion of lignocellulosic residues by Agrocybe cylindracea and Pleurotus ostreatus mushroom fungi - assessment of their effect on the final product and spent substrate properties. Food Chem. 2014;161:127-135.
  5. Correa RCG, Brugnari T, Bracht A, et al. Biotechnological, nutritional and therapeutic uses of Pleurotus spp. (Oyster mushroom) related with its chemical composition: a review on the past decade findings. Trends Food Sci Tech. 2016;50:103-117.
  6. Carrasco-Gonzalez JA, Serna-Saldivar SO, Gutierrez-Uribe JA. Nutritional composition and nutraceutical properties of the Pleurotus fruiting bodies: potencial use as food ingredient. J Food Compos Anal. 2017;58:69-81.
  7. Fernandes A, Barros L, Martins A, et al. Nutritional characterisation of Pleurotus ostreatus (Jacq. ex Fr.) P. Kumm. produced using paper scraps as substrate. Food Chem. 2015;169:396-400.
  8. Li H, Zhang Z, Li M, et al. Yield, size, nutritional value, and antioxidant activity of oyster mushrooms grown on perilla stalks. Saudi J Biol Sci. 2017;24:347-354.
  9. Meng F, Yang S, Wang X, et al. Reclamation of Chinese herb residues using probiotics and evaluation of their beneficial effect on pathogen infection. J Infect Public Health. 2017;10:749-754.
  10. Yang J, Qiu K. Development of high surface area mesoporous activated carbons from herb residues. Chem Eng J. 2011;167:148-154.
  11. Zhao S, Zhou T. Biosorption of methylene blue from wastewater by an extraction residue of Salvia miltiorrhiza Bge. Bioresour Technol. 2016;219:330-337.
  12. Guo F, Dong Y, Dong L, et al. An innovative example of herb residues recycling by gasification in a fluidized bed. Waste Manage. 2013;33:825-832.
  13. Gupta A, Sharma S, Saha S, et al. Yield and nutritional content of Pleurotus sajor caju on wheat straw supplemented with raw and detoxified mahua cake. Food Chem. 2013;141:4231-4239.
  14. Yang W, Guo F, Wan Z. Yield and size of oyster mushroom grown on rice/wheat straw basal substrate supplemented with cotton seed hull. Saudi J Biol Sci. 2013;20:333-338.
  15. Liu Y, Chen D, You Y, et al. Nutritional composition of boletus mushrooms from Southwest China and their antihyperglycemic and antioxidant activities. Food Chem. 2016;211:83-91.
  16. Association of Official Analytical Chemists. Official methods of analysis. 16th ed. Arlington (VA): Association of Official Analytical Chemists; 1995.
  17. Liang C, Wu C, Lu P, et al. Biological efficiency and nutritional value of the culinary-medicinal mushroom Auricularia cultivated on a sawdust basal substrate supplement with different proportions of grass plants. Saudi J Biol Sci. 2016. DOI:10.1016/j.sjbs.2016.10.017
  18. GB 5009.124 -2016. National food safety standards in food amino acids. Beijing: Standardization Administration of the People's Republic of China; 2016.
  19. Heleno SA, Barros L, Martins A, et al. Nutritional value, bioactive compounds, antimicrobial activity and bioaccessibility studies with wild edible mushrooms. LWT Food Sci Technol. 2015c;63:799-806.
  20. Liu Y, Sun J, Luo Z, et al. Chemical composition of five wild edible mushrooms collected from Southwest China and their antihyperglycemic and antioxidant activity. Food Chem Toxicol. 2012;50:1238-1244.
  21. Narain R, Sahu RK, Kumar S, et al. Influence of different nitrogen rich supplements during cultivation of Pleurotus florida on maize cobs substrate. Environmentalist. 2009;29:1-7.
  22. Xu F, Li Z, Liu Y, et al. Evaluation of edible mushroom Oudemansiella canarii cultivation on different lignocellulosic substrates. Saudi J Biol Sci. 2016;23:607-613.
  23. Liang Z, Wu C, Shieh Z, et al. Utilization of grass plants for cultivation of Pleurotus citrinopileatus. Int Biodeter Biodegr. 2009;63:509-514.
  24. Chukwurah NF, Eze SC, Chiejina NV, et al. Correlation of stipe length, pileus width and stipe girth of oyster mushroom (Pleurotus ostreatus) grown in different farm substrates. J Agric Biotech Sustain Dev. 2013;5:54-60.
  25. Beluhan S, Ranogajec A. Chemical composition and non-volatile components of Croatian wild edible mushrooms. Food Chem. 2011;124:1076-1082.
  26. Manzi P, Gambelli L, Marconi S, et al. Nutrients in edible mushrooms: an inter-species comparative study. Food Chem. 1999;65:477-482.
  27. Mintesnot B, Ayalew A, Kebede A. Evaluation of biomass of some invasive weed species as substrate for oyster mushroom (Pleurotus spp.) cultivation. Pak J Biol Sci. 2014;17:213-219.
  28. Inyod T, Sassanarakit S, Payapanon A, et al. Selection of Macrocybe crassa mushroom for commercial production. Agric Nat Resour. 2016;50:186-191.
  29. Heleno SA, Barros L, Martins A, et al. Chemical composition, antioxidant activity and bioaccessibility studies in phenolic extracts of two Hericium wild edible species. LWT Food Sci Technol. 2015b;63:475-481.
  30. Alananbeh KM, Bouqellah NA, Al Kaff NS. Cultivation of oyster mushroom Pleurotus ostreatus on date-palm leaves mixed with other agro-wastes in Saudi Arabia. Saudi J Biol Sci. 2014;21:616-625.
  31. GB 2762-2017. National food safety standards in food contaminants. Beijing: Standardization Administration of the People's Republic of China; 2017.
  32. Srikram A, Supapvanich S. Proximate compositions and bioactive compounds of edible wild and cultivated mushrooms from Northeast Thailand. Agric Nat Resour. 2016;50:432-436.
  33. Heleno SA, Ferreira RC, Antonio AL, et al. Nutritional value, bioactive compounds and antioxidant properties of three edible mushrooms from Poland. Food Biosci. 2015a;11:48-55.
  34. Amic D, Davidovic-Amic D, Beslo D, et al. Structure-radical scavenging activity relationships of flavonoids. Croat Chem Acta. 2003;76:55-61.
  35. da Paz MF, Breyer CA, Longhi RF, et al. Determining the basic composition and total phenolic compounds of Pleurotus sajor-caju cultivated in three different substrates by solid state bioprocess. J Biotec Biodivers. 2012;3:11-14.
  36. Muthangya M, Mshandete AM, Amana MJ, et al. Nutritional and antioxidant analysis of Pleurotus HK 37 grown on Agave sisalana saline solid waste. Int J Res Biochem Biophys. 2014;4:5-12.
  37. Yang H, Zhou Z, He L, et al. Hepatoprotective and inhibiting HBV effects of polysaccharides from roots of Sophora flavescens. Int J Biol Macromol. 2017;108:744-752.
  38. Lee JH, Ko MJ, Chung MS. Subcritical water extraction of bioactive components from red ginseng (Panax ginseng C.A. Meyer). J Supercrit Fluid. 2018;133:177-183.
  39. Wang QH, Han N, Dai N, et al. The structural elucidation and antimicrobial activities of two isoflavane glycosides from Astragalus membranaceus (Fisch) Bge. var. mongholicus (Bge) Hsiao. J Mol Struct. 2014;1076:535-538.