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A report of 28 unrecorded bacterial species, phylum Bacteroidetes, in Korea

  • Maeng, Soohyun (Department of Public Health Sciences, Graduate School, Korea University) ;
  • Baek, Chaeyun (Department of Public Health Sciences, Graduate School, Korea University) ;
  • Bae, Jin-Woo (Department of Biology, Kyung Hee University) ;
  • Cha, Chang-Jun (Department of Biotechnology, Chung-Ang University) ;
  • Jahng, Kwang-Yeop (Department of Life Sciences, Chonbuk National University) ;
  • Joh, Ki-seong (Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies) ;
  • Kim, Wonyong (Department of Microbiology, Chung-Ang University College of Medicine) ;
  • Seong, Chi Nam (Department of Biology, Sunchon National University) ;
  • Lee, Soon Dong (Faculty of Science Education, Jeju National University) ;
  • Cho, Jang-Cheon (Department of Biological Sciences, Inha University) ;
  • Yi, Hana (Department of Public Health Sciences, Graduate School, Korea University)
  • Received : 2017.08.28
  • Accepted : 2018.02.26
  • Published : 2018.05.31

Abstract

In order to investigate indigenous prokaryotic species diversity in Korea, various environmental samples from diverse ecosystems were examined. Isolated bacterial strains were identified based on 16S rRNA gene sequences, and those exhibiting at least 98.7% sequence similarity with known bacterial species, but not reported in Korea, were selected as unrecorded species. 28 unrecorded bacterial species belonging to the phylum Bacteroidetes were discovered from various habitats including wastewater, freshwater, freshwater sediment, wet land, reclaimed land, plant root, bird feces, seawater, sea sand, tidal flat sediment, a scallop, marine algae, and seaweed. The unrecorded species were assigned to 18 different genera in five families: Flavobacterium, Epilithonimonas, Dokdonia, Gillisia, Flavicella, Chryseobacterium, Algibacter, Aquimarina, Lacinutrix, Gaetbulibacter, Cellulophaga, Tenacibaculum, and Maribacter of Flavobacteriaceae, Dyadobacter of Cytophagaceae, Draconibacterium of Draconibacterium_f, Sunxiuqinia of Prolixibacteraceae, and Fulvivirga of Fulvivirga_f. The selected isolates were subjected to further taxonomic characterization including analysis of Gram reaction, cellular and colonial morphology, biochemical activities, and phylogenetic trees. Descriptive information of the 28 unrecorded species is provided.

Acknowledgement

Grant : The Survey of Korean Indigenous Species

Supported by : National Institute of Biological Resources

References

  1. Bernardet, J.-F. 2015. Flavobacteriales ord. nov. Bergey's Manual of Systematics of Archaea and Bacteria. W. B. Whitman. New York, Springer.
  2. Euzeby, J. 2012. Validation List no. 143. Int. J. Syst. Evol. Microbiol. 62:1-4. https://doi.org/10.1099/ijs.0.039487-0
  3. Jeon, Y.S., K. Lee, S.C. Park, B.S. Kim, Y.J. Cho, S.M. Ha and J. Chun. 2014. EzEditor: a versatile sequence alignment editor for both rRNA- and protein-coding genes. Int J Syst Evol Microbiol 64:689-691. https://doi.org/10.1099/ijs.0.059360-0
  4. Kämpfer, P. 2015. Sphingobacteriia class. nov. Bergey's Manual of Systematics of Archaea and Bacteria. W. B. Whitman. New York, Springer.
  5. Krieg, N.R. 2015. Bacteroidia class. nov. Bergey's Manual of Systematics of Archaea and Bacteria. W. B. Whitman. New York, Springer.
  6. Krieg, N.R., W. Ludwig, J. Euzeby and W.B. Whitman. 2010. Phylum XIV. Bacteroidetes phyl. nov. Bergey's Manual of Systematic Bacteriology. N. R. Krieg, J. T. Staley, D. R. Brown et al. New York, Springer. 4:25.
  7. Nakagawa, Y. 2015. Cytophagia class. nov. Bergey's Manual of Systematics of Archaea and Bacteria. W. B. Whitman. New York, Springer.
  8. Tamura, K., G. Stecher, D. Peterson, A. Filipski and S. Kumar. 2013. MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Mol. Biol. Evol. 30:2725-2729. https://doi.org/10.1093/molbev/mst197
  9. Thomas, F., J.H. Hehemann, E. Rebuffet, M. Czjzek and G. Michel. 2011. Environmental and gut bacteroidetes: the food connection. Front. Microbiol. 2:93.
  10. Yoon, S.H., S.M. Ha, S. Kwon, J. Lim, Y. Kim, H. Seo and J. Chun. 2017. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int. J. Syst. Evol. Microbiol. 67:1613-1617. https://doi.org/10.1099/ijsem.0.001755