Two combined amino acids promote sleep activity in caffeine-induced sleepless model systems

  • Hong, Ki-Bae (Department of Biological Sciences and Environmental Sciences Program, Southern Illinois University-Edwardsville) ;
  • Park, Yooheon (Dongguk University Research Institute of Biotechnology and Medical Converged Science, Dongguk University) ;
  • Suh, Hyung Joo (Department of Public Health Sciences, Korea University)
  • Received : 2017.10.25
  • Accepted : 2018.03.13
  • Published : 2018.06.01


BACKGROUND/OBJECTIVES: The aim of this study was to evaluate the biological and sleep-promoting effects of combined ${\gamma}$-aminobutyric acid (GABA) and 5-hydroxytryptophan (5-HTP) using caffeine-induced sleepless fruit flies, ICR mice, and Sprague-Dawley rats. MATERIALS/METHODS: Video-tracking analysis was applied to investigate behavioral changes of Drosophila melanogaster. Pentobarbital-induced sleep test and electroencephalogram (EEG) patterns were used for analysis of sleep latency, duration, and quantity and quality of sleep in vertebrate models. RESULTS: Administration of combined GABA/5-HTP could significantly reverse the caffeine induced total distance of flies (P < 0.001). Also, individually administered and combined GABA/5-HTP significantly increased the total sleeping time in the caffeine-induced sleepless ICR mice (P < 0.001). In the caffeine-induced sleepless SD-rats, combined GABA/5-HTP showed significant differences in sleep quality between individual amino acid administrations (P < 0.05). CONCLUSIONS: Taken together, we identified inhibitory effects of combined GABA/5-HTP in locomotor activity, sleep quantity and quality in caffeine-induced sleepless models, indicating that combined GABA/5-HTP may be effective in patients with insomnia by providing sufficient sleep.




Supported by : Korea Institute for Advancement of Technology (KIAT)


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