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Study on Molecular Phylogenetics of Korean Arisaema Species Based on Universal DNA Barcodes

범용성 DNA 바코드 분석 기반 한국산 천남성속(Arisaema) 식물의 분자계통학적 연구

  • Noh, Pureum (K-herb Research Center, Korea Institute of Oriental Medicine) ;
  • Han, Kyeongsuk (K-herb Research Center, Korea Institute of Oriental Medicine) ;
  • Kim, Wook Jin (K-herb Research Center, Korea Institute of Oriental Medicine) ;
  • Yang, Sungyu (K-herb Research Center, Korea Institute of Oriental Medicine) ;
  • Choi, Goya (K-herb Research Center, Korea Institute of Oriental Medicine) ;
  • Ko, Sung Chul (Department of Biological Science and Biotechnology) ;
  • Moon, Byeong Cheol (K-herb Research Center, Korea Institute of Oriental Medicine)
  • 노푸름 (한국한의학연구원 K-herb 연구단) ;
  • 한경숙 (한국한의학연구원 K-herb 연구단) ;
  • 김욱진 (한국한의학연구원 K-herb 연구단) ;
  • 양선규 (한국한의학연구원 K-herb 연구단) ;
  • 최고야 (한국한의학연구원 K-herb 연구단) ;
  • 고성철 (한남대학교 생명나노과학대학 생명시스템과학과) ;
  • 문병철 (한국한의학연구원 K-herb 연구단)
  • Received : 2017.10.13
  • Accepted : 2018.01.19
  • Published : 2018.02.28

Abstract

Molecular phylogenetic analysis was conducted to evaluate the taxonomic relationships of genus Arisaema L. distributed in Korea and the molecular phylogenetic characteristics of three authentic Arisaema species for the herbal medicine Arisaematis Rhizoma (the rhizomes of A. amurense, A. heterophyllum, and A. erubescens). The sequences of three DNA barcodes (rDNA-ITS, matK, and rbcL) were analyzed using 50 samples of nine taxa consisted of eight Korean and one Chinese Arisaema with one outgroup (Dracunculus vulgaris). Both individual and combined phylogenetic analyses of three DNA barcode sequences revealed that the treated nine taxa are independently classified into six distinct clades (Clade I, A. amurense f. amurense and A. amurense f. serratum; Clade II, A. serratum and A. takesimense; Clade III, A. ringens; Clade IV, A. erubescens; Clade V, A. heterophyllum; Clade VI, A. thunbergii subsp. thunbergii and A. thunbergii subsp. geomundoense). These six clades were reasonably divided into three individual sections, Pedatisecta, Sinarisaema, and Tortuosa. Futhermore, the results of comparative DNA barcode sequences analyses provided a significant information for the taxonomic reconsideration of Arisaema L. at the specific and intraspecific level. However, we could not confirm the taxonomic characteristics or identity among the three authentic medicinal species through the molecular phylogenetic analyses of genus Arisaema L. for Arisaematis Rhizoma.

국내에 분포하는 천남성속의 계통학적 유연관계와 한약재 천남성(Arisaematis Rhizoma)으로 사용되는 천남성 3종(둥근잎천남성, 두루미천남성, 일파산남성)에 대한 분류학적 특징을 분석하기 위하여 천남성속 식물에 대한 분자계통학적 연구를 수행하였다. 3개의 범용성 DNA 바코드(ITS, matK, rbcL) 염기서열을 이용하여 국내 분포 천남성속 8분류군과 중국에 분포하는 약전수재 종 1분류군을 포함하는 9종 50개 시료와 같은 과의 Dracunculus vulgaris를 군외군으로 하여 유연관계를 분석하였다. 3개의 개별 DNA 바코드 염기서열과 이들을 유합한 염기서열로 계통학적 유연관계를 분석한 결과, 천남성속의 9 분류군은 6개의 독립적인 분계조를 형성하며 구별되었으며(Clade I, 둥근잎천남성 및 천남성; Clade II, 점박이천남성 및 섬남성; Clade III, 큰천남성; Clade IV, 일파산남성; Clade V, 두루미천남성; Clade VI, 무늬천남성 및 거문천남성), 이들 6개의 분계조는 각각 Pedatisecta절, Sinarisaema절, 및 Tortuosa절로 분류되었다. 또한 이들 DNA 바코드 구간의 비교 결과는 천남성속 식물의 종 및 종 이하 분류 단위의 분류학적 재검토의 필요성에 대한 중요한 정보를 제공하였다. 하지만 대한민국약전에 수재되어 한약재로 사용가능한 3종의 천남성 기원종의 종내 분류학적 연관성이나 분자계통학적 특징은 확인되지 않았다.

Keywords

Acknowledgement

Grant : 기원검증 체계 구축을 통한 한약자원 국내생산 기반기술 개발

Supported by : 한국한의학연구원

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