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Protection of Radiation-Induced DNA Damage by Functional Cosmeceutical Poly-Gamma-Glutamate

  • Oh, Yu-Jin (Department of Bio and Fermentation Convergence Technology, BK21 PLUS Project, Kookmin University) ;
  • Kwak, Mi-Sun (Department of Bio and Fermentation Convergence Technology, BK21 PLUS Project, Kookmin University) ;
  • Sung, Moon-Hee (Department of Bio and Fermentation Convergence Technology, BK21 PLUS Project, Kookmin University)
  • Received : 2017.12.07
  • Accepted : 2018.01.22
  • Published : 2018.04.28

Abstract

This study compared the radioprotective effects of high-molecular-weight poly-gamma-glutamate (${\gamma}-PGA$, average molecular mass 3,000 kDa) and a reduced form of glutathione (GSH, a known radioprotector) on calf thymus DNA damage. The radiation-induced DNA damage was measured on the basis of the decreased fluorescence intensity after binding the DNA with ethidium bromide. All the experiments used $^{60}Co$ gamma radiation at 1,252 Gy, representing 50% DNA damage. When increasing the concentration of ${\gamma}-PGA$ from 0.33 to $1.65{\mu}M$, the DNA protection from radiation-induced damage also increased, with a maximum of 87% protection. Meanwhile, the maximal DNA protection when increasing the concentration of GSH was only 70%. Therefore, ${\gamma}-PGA$ exhibited significant radioprotective effects against gamma irradiation.

Acknowledgement

Supported by : Korea Institute for Advancement of Technology (KIAT)

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