Human amnion-derived mesenchymal stem cells induced osteogenesis and angiogenesis in human adipose-derived stem cells via ERK1/2 MAPK signaling pathway

  • Wang, Yuli (Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University) ;
  • Chen, Xichen (Analysis Center, Nanjing Medical University) ;
  • Yin, Ying (Nanjing Stomatological Hospital, Medical School of Nanjing University) ;
  • Li, Song (Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University)
  • Received : 2018.01.11
  • Accepted : 2018.02.06
  • Published : 2018.04.30


Mesenchymal stem cells (MSCs) have shown great potential in treating bone deficiency. Human adipose-derived stem cells (HASCs) are multipotent progenitor cells with multi-lineage differentiation potential. Human amnion-derived mesenchymal stem cells (HAMSCs) are capable of promoting osteogenic differentiation of MSCs. In this study, we investigated the effect of HAMSCs on HASCs by a transwell co-culture system. HAMSCs promoted proliferation, osteogenic differentiation, angiogenic potential and adiponectin (APN) secretion of HASCs. Moreover, the positive effect of HAMSCs was significantly inhibited by U0126, a highly selective inhibitor of extracellular signaling-regulated kinase 1/2 (ERK1/2) mitogen-activated protein kinase (MAPK) signaling pathway. These observations suggested that HAMSCs induced bone regeneration in HASCs via ERK1/2 MAPK signaling pathway.


Supported by : Jiangsu Higher Education Institutions


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