- Volume 31 Issue 6
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Applications of capacitation status for litter size enhancement in various pig breeds
- Kwon, Woo-Sung (Department of Animal Biotechnology, Kyungpook National University) ;
- Shin, Dong-Ha (Department of Animal Science & Technology, ChungAng University) ;
- Ryu, Do-Yeal (Department of Animal Science & Technology, ChungAng University) ;
- Khatun, Amena (Department of Animal Science & Technology, ChungAng University) ;
- Rahman, Md Saidur (Department of Animal Science & Technology, ChungAng University) ;
- Pang, Myung-Geol (Department of Animal Science & Technology, ChungAng University)
- Received : 2017.10.16
- Accepted : 2017.12.02
- Published : 2018.06.01
Objective: Several studies have reported the development of new molecular methods for the prognosis and diagnosis of male fertility based on biomarkers aimed at overcoming the limitations of conventional male fertility analysis tools. However, further studies are needed for the field application of these methods. Therefore, alternative methods based on existing semen analysis methods are required to improve production efficiency in the animal industry. Methods: we examined the possibility of improving litter size in various pig breeds using combined Hoechst 33258/chlortetracycline fluorescence (H33258/CTC) staining. The correlation between field fertility and capacitation status by combined H33258/CTC staining in different ejaculates spermatozoa (n = 3) from an individual boar (20 Landrace, 20 Yorkshire, and 20 Duroc) was evaluated as well as overall accuracy. Results: The acrosome reacted (AR) pattern after capacitation (%) was positively correlated with the litter size of Landrace, Yorkshire, and Duroc pigs and the overall accuracy was 75%, 75%, and 70% in Landrace, Yorkshire, and Duroc pigs, respectively. The difference (
Supported by : Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET)
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