Antimicrobial and Antitumor Photodynamic Effects of Phleichrome from the Phytopathogenic Fungus Cladosporium Phlei

  • So, Kum-Kang (Departments of Molecular Biology and Bioactive Material Sciences, Institute for Molecular Biology and Genetics Chonbuk National University) ;
  • Chun, Jeesun (Departments of Molecular Biology and Bioactive Material Sciences, Institute for Molecular Biology and Genetics Chonbuk National University) ;
  • Kim, Dae-Hyuk (Departments of Molecular Biology and Bioactive Material Sciences, Institute for Molecular Biology and Genetics Chonbuk National University)
  • Received : 2018.10.29
  • Accepted : 2018.11.13
  • Published : 2018.12.31


Fungal perylenequinones have photodynamic activity and are promising photosensitizers for photodynamic therapy (PDT). Here, we investigated the bactericidal and antitumor activities of phleichrome from the fungal perylenequinone family in vitro. Photodynamic bactericidal activity of phleichrome was analyzed by agar-well diffusion method under dark and illuminated conditions. The photodynamic antitumor activity of phleichrome was analyzed in MCF-7, HeLa, SW480, and HepG2 human cancer cell lines using in vitro cytotoxicity assays. Photodynamic bactericidal activities against Gram-negative and Gram-positive bacteria were species-specific. Antitumor activity against all tumor cell lines increased under the illuminated condition. Depending on the results of the analyses, Phleichrome has potential for further drug development related to its antibacterial and antitumor activities.


Supported by : NRF


  1. Pra_zmo EJ, Kwasny M, Lapinski M, et al. Photodynamic therapy as a promising method used in the treatment of oral diseases. Adv Clin Exp Med. 2016;25:799-807.
  2. Ackroyd R, Kelty C, Brown N, et al. The history of photodetection and photodynamic therapy. Photochem Photobiol. 2001;74:656-669.<0656:THOPAP>2.0.CO;2
  3. Masiera N, Bojarska A, Gawryszewska I, et al. Antimicrobial photodynamic therapy by means of porphycene photosensitizers. J Photochem Photobiol B Biol. 2017;174:84-89.
  4. Sperandio FF, Huang YY, Hamblin MR. Antimicrobial photodynamic therapy to kill Gram-negative bacteria. Recent Pat Antiinfect Drug Discov. 2013;8:108-120.
  5. Baltazar LM, Ray A, Santos DA, et al. Antimicrobial photodynamic therapy: an effective alternative approach to control fungal infections. Front Microbiol. 2015;6:202.
  6. Yoshihara T, Shimanuki T, Araki T, et al. Phleichrome: a new phytotoxic compound produced by Cladosporium phlei. Arg Biol Chem. 1975;39:1683-1684.
  7. So KK, Jo IS, Chae MS, et al. Improved production of phleichrome from the phytopathogenic fungus Cladosporium phlei using synthetic inducers and photodynamic ROS production by phleichrome. J Biosci Bioeng. 2015;119:289-296.
  8. Gnanamanickam SS, Smith DA. Selective toxicity of isoflavonoid phytoalexins to Gram-positive bacteria. Phytopathology. 1980;70:894-896.
  9. So Y, Lee SY, Han AR, et al. Rosmarinic acid methyl ester inhibits LPS-induced NO production via suppression of MyD88- dependent and -independent pathways and induction of HO-1 in RAW 264.7 cells. Molecules. 2016;21:1083.
  10. Zhu TC, Finlay JC. The role of photodynamic therapy (PDT) physics. Med Phys. 2008;35:3127-3136.
  11. Rkein AM, Ozog DM. Photodynamic therapy. Dermatol Clin. 2014;32:415-425.
  12. Abrahamse H, Hamblin MR. New photosensitizers for photodynamic therapy. Biochem J. 2016;473:347-364.
  13. O’Connor AE, Gallagher WM, Byrne AT. Porphyrin and nonporphyrin photosensitizers in oncology: preclinical and clinical advances in photodynamic therapy. Photochem Photobiol. 2009;85:1053-1074.
  14. Alves E, Costa L, Carvalho CM, et al. Charge effect on the photoinactivation of Gram-negative and Gram-positive bacteria by cationic meso-substituted porphyrins. BMC Microbiol. 2009;9:70.
  15. Merchat M, Spikes JD, Bertoloni G, et al. Studies on the mechanism of bacteria photosensitization by meso-substituted cationic porphyrins. J Photochem Photobiol B Biol. 1996;35:149-157.
  16. Caminos DA, Spesia MB, Durantini EN. Photodynamic inactivation of Escherichia coli by novel meso-substituted porphyrins by 4-(3-N,N,N-trimethylammoniumpropoxy)phenyl and 4-(trifluoromethyl)phenyl groups. Photochem Photobiol Sci. 2006;5:56-65.
  17. Banfi S, Caruso E, Buccafurni L, et al. Antibacterial activity of tetraaryl-porphyrin photosensitizers: an in vitro study on Gram negative and Gram positive bacteria. J Photochem Photobiol B. 2006;85:28-38.