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Immobilization of Lysozyme from Hen Egg by Crosslinking Method onto Chitosan Non-woven

키토산 섬유를 담체로 이용한 라이소자임 효소의 고정화

  • Lee, So Hee (Department of Clothing and Textiles/Research Institute of Natural Science, Gyeongsang National University)
  • 이소희 (경상대학교 의류학과/기초과학연구소)
  • Received : 2018.11.27
  • Accepted : 2018.12.11
  • Published : 2018.12.27

Abstract

Immobilization of lysozyme on chitosan non-woven using glutaraldehyde(GA) was investigated. For this, 100 % chitosan non-woven was prepared as novel support for the enzyme immobilization. In addition, free lysozyme activity was examined depending on various pH and temperature by measuring time. Moreover, the optimum immobilization conditions depending on various pH, temperature, immobilization time and lysozyme concentration was evaluated. In addition, thermal stability and storage stability of immobilized lysozyme were measured. The characteristics of immobilized lysozyme was examined by FT-IR, surface morphology, and MTT assay. The results are follows: the optimal immobilization of lysozyme were pH 7.0, $25^{\circ}C$, lysozyme concentration 1.5 mg/ml, immobilization time 240 min. The immobilized lysozyme showed higher thermal stability than the free trypsin. The immobilized lysozyme activity was retained 80 % of its initial activity at $4^{\circ}C$ over 30 days of storage. The lysozyme was immobilized effectively on chitosan non-woven by observation of surface morphology.

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Figure 1. The effects of pH on the free lysozyme activity depending on time.

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Figure 2. The effect of temperature on the free lysozyme activity depending on time.

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Figure 3. The effects of pH on the immobilized lysozyme activity.

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Figure 4. The effects of temperature on the immobilized lysozyme activity.

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Figure 5.The effects of immobilization time on the immobilized lysozyme activity.

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Figure 7. The thermal stabilities of immobilized lysozyme.

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Figure 6. The effects of lysozyme concentration on the immobilized lysozyme activity.

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Figure 8.Storage stabilities of immobilized lysozyme.

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Figure 9. Antimicrobial effects of the lysozyme immobilized chitosan non-woven.

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Figure 10. Cell viability of the chitosan non-woven.

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Figure 11. FT-IR spectrometer of chitosan non-woven.

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Figure 12. FE-SEM analysis of the chitosan non-woven.

Table 1. Characteristics of chitosan non-woven

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Table 2. Properties of enzyme

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Acknowledgement

Supported by : 한국연구재단

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