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Protective Effect of 6 Food Mixed-Extract on the CCl4-Induced Hepatic Damage in Rats

6종 식품의 혼합추출물이 사염화탄소로 유도된 흰쥐 간 손상에 미치는 보호효과

  • 황보미향 (계명문화대학교 식품영양조리학부) ;
  • 예춘정 (계명문화대학교 보건학부)
  • Received : 2018.10.10
  • Accepted : 2018.11.08
  • Published : 2018.12.31

Abstract

The objective of this study was to examine the biochemical parameters of hepatic function such as serum level of ALT (alanine aminotransferase), AST (aspartate aminotransferase), ALP (alkaline phosphatase), LDH (lactate dehydrogenase), and content of TG (triglyceride) and cholesterol, and tissue immunological changes of the $CCl_4$-treated rats with administration of the mixed sample extract (MSE). The liver weight in $CCl_4$-administered experimental control group (EC) was slightly higher than that of normal control (NC) group. Hepatic damage parameters (ALT, AST, ALP, LDH & TG) in serum of the EC group were significantly higher than those in serum of the NC and silymarin-treated positive control (PC) group. On the other hand, these hepatic damage parameters of MSE-treated experimental (E1 & E2) groups were significantly lower than those of EC group. The number of WBC, neutrophils, lymphocytes and platelets, and the contents of hemoglobin, and hematocrit in EC group were significantly higher than those of NC group. However, the number of WBC and lymphocytes in E1 and E2 groups were significantly lower than those of EC group. Also, the collagen developmental areas in the liver of NC and PC groups by hepatic immuno-histological findings were found slightly positive. Whereas, hepatic fibrous developmental tissue of EC group was strongly positive brown color band, those of E1 & E2 groups were decreased. Therefore, it was concluded that the induction of hepatic fibrous tissue activation had a preventive effect of MSE against the $CCl_4$-induced hepatic damage in rats. However, further study is needed in this filed.

Keywords

liver;hematological;tetrachlorid;biochemical;hepatic damage;immunological

Table 1. Water intake, food intake, body weight gain food and efficiency ratio of CCl4 in rats treated with mixed sample extract (MSE)

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Table 2. Effect of mixed sample extract (MSE) on the organ weight of the CCl4-induced hepatic damage in rats

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Table 3. Effect of mixed sample extract (MSE) on the serum levels of ALT, AST, ALP, LDH and the contents of triglyceride (TG) and cholesterol (CHOL) of the CCl4-induced hepatic damage in rats

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Table 4. Changes of hematological parameters in CCl4-induced hepatic damage of rats treated with mixed sample extract (MSE)

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Table 5. Comparison of SMA protein expression in rat live tissue treated with mixed sample extract (MSE)

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