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Anti-inflammatory activity Effects of Mori Folium Water Extracton IL-1α, IL-6 and IL-10 on mouse macrophages

상엽 추출물이 마우스 대식세포의 IL-1α, IL-6, IL-10에 대한 항염활성 연구

  • Park, Young Sik (Department of Alternative medicine, Nambu University) ;
  • Han, Hyo-Sang (Department of Health Administration, Joongbu University)
  • 박영식 (남부대학교 대체의학과) ;
  • 한효상 (중부대학교 보건행정학과)
  • Received : 2018.09.19
  • Accepted : 2018.11.20
  • Published : 2018.11.28

Abstract

This study was conducted to investigate of Mori Folium Water Extract (MF) on anti-inflammation activity. MF Water extracts after 24 houres cultivation were examined to ascertain the cell viability of mouse macrophage RAW 264.7 cells. The influence of the Water extracts in RAW 264.7 macrophage cells treated with LPS was investigated. nitric oxide (NO) production, nterleukin$(IL)-1{\alpha}$ IL-6 and IL-10 increased generation of cytokines. mouse macrophage RAW 264.7 cells cell viability changes were no decreas after MTT assay of MF Water extract. The MF water extracts inhibited NO generation caused by LPS in the macrophages over $25{\mu}g/mL$. The MF water extracts increased in the control group the $IL-1{\alpha}$ and IL-6 activation generated by LPS in the macrophages over $50{\mu}g/mL$. Accordingly, it was found that different MF water extract concentrations significantly influenced certain anti-inflammation activities in RAW 264.7 macrophage cells. The results of this study are expected to be highly applicable to health - friendly functional materials. Further studies are needed to confirm the signaling pathways associated with anti-inflammation of macrophages through continuous studies.

Keywords

Mori Folium;Nitric oxide;Anti-inflammatory effect;Macrophage;Cytokine

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Fig. 1. Effect of MF on cell viability in RAW 264.7 macrophage cells incubated for 24 hrs. Results are the mean ± SD of three independent experiments.

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Fig. 2. Effect of MF on the nitric oxide production in RAW 264.7 macrophage cells were incubated for 24 hrs. treated with LPS; 1 ㎍ /mL. Results are the mean ± SD of three independent experiments.

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Fig. 3. Effect of MF on the IL-1α production in RAW 264.7 macrophage cells were incubated for 24 hrs. treated with LPS; 1 ㎍/mL. Results are the mean ± SD of three independent experiments.

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Fig. 4. Effect of MF on the IL-6 production in RAW 264.7 macrophage cells were incubated for 24 hrs. treated with LPS; 1 ㎍/mL. Results are the mean ± SD of three independent experiments.

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Fig. 5. Effect of MF on the IL-10 production in RAW 264.7 macrophage cells were incubated for 24 hrs. treated with LPS; 1 ㎍/mL. Results are the mean ± SD of three independent experiments.

Table 1. Effect of MF on IL-6 Production in Raw 264.7 Cells Treated with LPS

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Table 2. Effect of MF on IL-10 Production in Raw 264.7 Cells Treated with LPS

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