Fig. 1. Effect of different culture techniques on morphological changes of intramuscular adipocytes and muscle cells. Intramuscular preadipocytes (IP) and muscle satellite cells (MSC) obtained from Hanwoo were grown at 37℃ under a humidified atmosphere of 5% CO2. Upon reaching approximately 70% of confluency, the growth medium was replaced with 2% fetal bovine serum/Dulbecco's modified Eagles medium (FBS/DMEM) for both IP and MSC. Intramuscular adipocytes and muscle cells were co-cultured using a 0.4 μm insert membrane. Black arrows indicate lipid droplets for adipocytes and myotubes for muscle cells, respectively. Magnification = 100 x.
Table 1. Effect of different culture techniques on differentiation of muscle cells and intramuscular adipocytes isolated from Korean native cattlez.