Effects of dietary fermented spent coffee ground on nutrient digestibility and nitrogen utilization in sheep

  • Choi, Yongjun ;
  • Rim, Jong-su ;
  • Na, Youngjun ;
  • Lee, Sang Rak
  • Received : 2017.09.06
  • Accepted : 2017.10.23
  • Published : 2018.03.01


Objective: The objective of the study was to determine the effect of fermented spent coffee ground (FSCG) on nutrient digestibility and nitrogen utilization in sheep. Methods: Fermentation of spent coffee ground (SCG) was conducted using Lactobacillus plantrum. Fermentation was performed at moisture content of 70% and temperature of $39^{\circ}C$ with anaerobic air tension for 48 h. Four adult rams (initial body weight = $56.8{\pm}0.4kg$) were housed in a respiration-metabolism chamber and the treatments were: i) control (Basal diet; 0% SCG or FSCG), ii) 10% level of SCG, iii) 10% level of FSCG, and iv) 20% level of FSCG in $4{\times}4$ Latin square design. Each dietary experiment period lasted for 18-d with a 14-d of adaptation period and a 4-d of sample collection period. Results: In SCG fermentation experimental result, acid detergent insoluble nitrogen (ADIN) concentration of FSCG (64.5% of total N) was lower than that of non-fermented SCG (78.8% of total N). Digestibility of dry matter and organic matter was similar among treatment groups. Although crude protein (CP) digestibility of the control was greater than FSCG groups (p<0.05), the 10% FSCG group showed greater CP digestibility and nitrogen retention than non-fermented 10% SCG group (p<0.05). Body weight gain and average daily gain were linearly decreased with increasing FSCG feeding level (p<0.05). When the feeding level of FSCG was increased, water intake was linearly increased (p<0.05). With an increasing FSCG level, dry matter intake did not differ among groups, although the gain to feed ratio tended to decrease with increasing level of FSCG (p<0.10). Conclusion: Microbial fermentation of SCG can improve protein digestibility, thereby increasing CP digestibility and nitrogen utilization in sheep. Fermentation using microorganisms in feed ingredients with low digestibility could have a positive effect on improving the quality of raw feed.


Feed Intake;Fermented Spent Coffee Ground;Nutrient Digestibility;Nitrogen Utilization;Sheep


  1. Machado EMS, Rodriguez-Jasso RM, Teixeira JA, Mussatto I. Growth of fungal strains on coffee industry residues with removal of polyphenolic compounds. Biochem Eng J 2012;60:87-90.
  2. Campos-Vega R, Loarca-Pina G, Vergara-Castaneda HA, Oomah BD. Spent coffee grounds: A review on current research and future prospects. Trends Food Sci Technol 2015;45:24-36.
  3. Vignoli JA, Bassoli DG, Benassi MT. Antioxidant activity, polyphenols, caffeine and melanoidins in soluble coffee: the influence of processing conditions and raw material. Food Chem 2011;124:863-8.
  4. Campbell TW, Bartley EE, Bechtle RM, Dayton AD. Coffee grounds. I. Effects of coffee grounds on ration digestibility and diuresis in cattle, on in vitro rumen fermentation, and on rat Growth. J Dairy Sci 1976;59:1452-60.
  5. Seo J, Jung JK, Seo S. Evaluation of nutritional and economic feed values of spent coffee grounds and Artemisia princeps residues as a ruminant feed using in vitro ruminal fermentation. Peer J 2015;3:e1343.
  6. Givens DI, Barber WP. In vivo evaluation of spent coffee grounds as a ruminant feed. Agric Wastes 1986;18:69-72.
  7. Pond WG, Church DC, Pond KR, others. Basic animal nutrition and feeding. 4 th ed. Wiley india Pvt. Ltd., New Delhi, India: John Wiley and Sons; 1995.
  8. de Brito CBM, Felix AP, de Jesus RM, et al. Digestibility and palatability of dog foods containing different moisture levels, and the inclusion of a mould inhibitor. Anim Feed Sci Technol 2010;159:150-5.
  9. Baumont R. Palatability and feeding behaviour in ruminants. A review. Ann Zootech 1996;45:385-400.
  10. Ando S, Ishida M, Oshio S, Tanaka O. Effects of isolated and commercial lactic acid bacteria on the silage quality, digestibility, voluntary intake and ruminal fluid characteristics. Asian-Australas J Anim Sci 2006;19:386-9.
  11. Agarwal R, Lata S, Gupta M, Singh P. Removal of melanoidin present in distillery effluent as a major colorant: A Review. J Environ Biol 2010;31:521-8.
  12. Tondee T, Sirianuntapiboon S. Decolorization of molasses wastewater by Lactobacillus plantarum No. PV71-1861. Bioresour Technol 2008;99:6258-65.
  13. Sneasth PHA, Mair NS, Sharpe ME, Holt JG. Bergey's manual of systematic bacteriology, Volume 2. New York, NY: Springer US; 2005.
  14. Li DH, Kim BG, Lee SR. A respiration-metabolism chamber system for measuring gas emission and nutrient digestibility in small ruminant animals. Rev Colomb Cienc Pecu 2010;23:444-50.
  15. National Research Council. Nutrient requirements of small ruminants. Washington, DC, USA: National Academy Press; 2007.
  16. Latimer JW, Horwitz W. Official methods of analysis of AOAC International. 18th ed. Washington, DC, USA: AOAC International Press; 2005.
  17. Soest PJV, Robertson JB, Lewis BA. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. J Dairy Sci 1991;74:3583-97.
  18. Licitra G, Hernandez TM, Van Soest PJ. Standardization of procedures for nitrogen fractionation of ruminant feeds. Anim Feed Sci Technol 1996;57:347-58.
  19. Cai Y, Benno Y, Ogawa M, Kumai S. Effect of applying lactic acid bacteria isolated from forage crops on fermentation characteristics and aerobic deterioration of Silage. J Dairy Sci 1999;82:520-6.
  20. Chaney AL, Marbach EP. Modified reagents for determination of urea and ammonia. Clin Chem 1962;8:130-2.
  21. Fussell RJ, McCalley DV. Determination of volatile fatty acids ($C_{2}-C_{5}$) and lactic acid in silage by gas chromatography. Analyst 1987;112:1213-6.
  22. Littell RC, Milliken GA, Stroup WW, Wolfinger RD, Schabenberger O. SAS for mixed models. Cary, NC, USA: Institute Inc; 2007.
  23. Bekedam EK. Coffee brew melanoidins: structural and functional poperties of brown-colored coffee compounds. [Ph. D. thesis]. Wageningen, Netherlands: Wageningen University; 2008.
  24. Givens DI, Barber WP. In vivo evaluation of spent coffee grounds as a ruminant feed. Agric Wastes 1986;18:69-72.
  25. Xu CC, Cai Y, Zhang JG, Ogawa M. Fermentation quality and nutritive value of a total mixed ration silage containing coffee grounds at ten or twenty percent of dry matter. J Anim Sci 2006;85:1024-9.
  26. Axelsson L, Ahrne S. Lactic acid bacteria. Applied microbial systematics. Dordrecht, The Netherlands: Springer; 2000. pp. 367-88.
  27. Seo JK, Kim SW, Kim MH, et al. Direct-fed microbials for ruminant animals. Asian-Australas J Anim Sci 2010;23:1657-67.
  28. Maughan RJ, Griffin J. Caffeine ingestion and fluid balance: a review. J Hum Nutr Diet 2003;16:411-20.
  29. Borrelli RC, Visconti A, Mennella C, Anese M, Fogliano V. Chemical characterization and antioxidant properties of coffee melanoidins. J Agric Food Chem 2002;50:6527-33.


Supported by : Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET)