Fig. 1. Ventx1.1 represses early and late neural genes in Xenopus whole embryo and animal cap explants.
Fig. 2. Genome-wide Xenopus ChIP-Seq of Ventx1.1 identifies two Ventx1.1 response cisacting elements (VRE1 and VRE2) on the zic3 promoter.
Fig. 3. Site-directed mutagenesis of VRE1 and VRE2 within the zic3 promoter-reporter construct abolishes the repressional activity of Ventx1.1. on the reporter.
Fig. 4. VRE1 mutation partially abolishes negative and positive effects of Xcad2 and FoxD5b-En, respectively.
Fig. 5. Schematic diagram depicting Ventx1.1 as a key protein in the neural inhibitory circuit of BMP4/Smad1/Xcad2/Ventx1.1 and the reciprocal inhibitory circuit of FoxD5b/Ventx1.1.
Table 1. Primers used for serially-deleted reporter gene constructs
Table 2. Primers used for RT-PCR amplification
Table 3. Primers used for site-directed mutagenesis gene constructs
Table 4. Primers used for ChIP-PCR assay