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DRG2 Deficiency Causes Impaired Microtubule Dynamics in HeLa Cells

  • Dang, Thao ;
  • Jang, Soo Hwa ;
  • Back, Sung Hoon ;
  • Park, Jeong Woo ;
  • Han, In-Seob
  • Received : 2018.03.15
  • Accepted : 2018.09.27
  • Published : 2018.12.31

Abstract

The developmentally regulated GTP binding protein 2 (DRG2) is involved in the control of cell growth and differentiation. Here, we demonstrate that DRG2 regulates microtubule dynamics in HeLa cells. Analysis of live imaging of the plus-ends of microtubules with EB1-EGFP showed that DRG2 deficiency (shDRG2) significantly reduced the growth rate of HeLa cells. Depletion of DRG2 increased 'slow and long-lived' subpopulations, but decreased 'fast and short-lived' subpopulations of microtubules. Microtubule polymerization inhibitor exhibited a reduced response in shDRG2 cells. Using immunoprecipitation, we show that DRG2 interacts with tau, which regulates microtubule polymerization. Collectively, these data demonstrate that DRG2 may aid in affecting microtubule dynamics in HeLa cells.

Keywords

DRG2;microtubule dynamics;tau

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Fig. 1. DRG2 depletion regulates the spatial organization of dynamic switching behavior of microtubules in HeLa cells.

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Fig. 2. Depletion of DRG2 enhances resistance to microtubule inhibitors.

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Fig. 3. Depletion of DRG2 enhances resistance to microtubule depolymerization induced by nocodazole.

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Fig. 4. DRG2 binds to tau and increases phosphorylation on tau at S202.

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Acknowledgement

Supported by : National Research Foundation of Korea (NRF)