Preparation of Surface Functionalized Gold Nanoparticles and their Lateral Flow Immunoassay Applications

표면 개질된 금나노입자의 제조 및 이의 측방유동면역 센서 응용

  • Kim, Dong Seok (Department of Chemical Engineering, Kangwon National University) ;
  • Choi, Bong Gill (Department of Chemical Engineering, Kangwon National University)
  • 김동석 (강원대학교 화학공학과) ;
  • 최봉길 (강원대학교 화학공학과)
  • Received : 2017.10.24
  • Accepted : 2017.12.13
  • Published : 2018.02.10


In this work, the surface of gold nanoparticles (AuNPs) was modified with small molecules including mercaptoundecanoic acid (MUA) and L-lysine for the development of highly sensitive lateral flow (LF) sensors. Uniformly sized AuNps were synthesized by a modified Turkevich-Frens method, showing an average size of $16.7{\pm}2.1nm$. Functionalized AuNPs were then characterized by transmission electron microscopy, UV-vis spectroscopy, X-ray photoelectron spectroscopy, and Fourier transform infrared spectroscopy. The stable conjugation of AuNPs and antibodies was obtained at pH 7.07 and the antibody concentration of $10{\mu}g/mL$. The functionalized AuNP-based LF sensor exhibited lower detection limit of 10 ng/mL for hepatitis B surface antigens than that of using the bare AuNP-based LF sensor (100 ng/mL).


gold nanoparticle;functionalization;hepatitis B surface antigen;lateral flow immunoassay


Supported by : 한국산업기술진흥원, 한국연구재단


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