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Effect of Youngyanggak-san against Thioacetamide Induced Acute Liver Damage in Rat

영양각산이 Thioacetamide 유발 간손상에 미치는 영향

  • Shin, Mi-Rae (Department of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Kim, Kyeong Jo (Department of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Kim, Soo Hyun (Department of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Lee, Ji-Hye (Department of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Kwon, O Jun (Gyeongbuk Institute for Regional Program Evaluation) ;
  • Roh, Seong-Soo (Department of Herbology, College of Korean Medicine, Daegu Haany University)
  • 신미래 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 김경조 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 김수현 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 이지혜 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 권오준 (경북지역사업평가단) ;
  • 노성수 (대구한의대학교 한의과대학 본초약리학교실)
  • Received : 2017.12.12
  • Accepted : 2018.01.15
  • Published : 2018.01.30

Abstract

Objectives : The current study is to evaluate the hepatoprotective effect of youngyanggak-san (YGS) on thioacetamide (TAA)-induced acute liver injury in rats. Methods : YGS is composed of Glycyrrhizae Radix, Asiasari Radix, Cimicifugae Rhizoma, Saigae Tataricae Cornu. While N-YGS (non-youngyanggak-san) doesn't include Saigae Tataricae Cornu. Two samples were administrated TAA together for 3 days. Thirty-six rats were divided into four groups. Rats except for the normal group were received TAA (200 mg/kg of body weight, I.P) were divided into three groups (n=9/group) : Group 1 (TAA only), Group 2 (TAA + 200 mg/kg YGS) and Group 3 (TAA + 200 mg/kg N-YGS). Acute liver damage confirmed using histological examination, The factors associated with oxidative stress and liver function activity measured in serum. Also, expressions of inflammation related proteins were investigated by western blot analysis. Results : Oxidative stress factors such as ROS and $ONOO^-$ in the Group 2 was manifested by a significant rise compared with Group 1. YGS markedly decreased the elevated ROS and $ONOO^-$. Furthermore, YGS significantly reduced the levels of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) The nuclear $factor-{\kappa}B$ ($NF-{\kappa}B$) activation induced by TAA led to increase both inflammatory mediators and cytokines. While YGS administration remarkably suppressed such the overexpression. In addition, the histopathological analysis showed that the liver tissue lesions were improved obviously in YGS treatment. Conclusion : YGS provided a hepatoprotective effect on acute liver damage through the suppression of oxidative stress. Especially, this effect enhanced markedly when Saigae Tataricae Cornu is included.

Keywords

Acknowledgement

Supported by : 한국한의학연구원

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