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Genome Size Estimation of the Two Wing Morphs of Vollenhovia emeryi (Hymenoptera: Myrmicinae)

에메리개미 (Vollenhovia emeryi Wheeler)의 날개이형체의 유전체 크기 추정

  • 노푸름 (이화여자대학교 에코과학부) ;
  • 박소연 (이화여자대학교 에코과학부) ;
  • 최재천 (이화여자대학교 에코과학부) ;
  • 정길상 (국립생태원)
  • Received : 2018.09.12
  • Accepted : 2018.11.08
  • Published : 2018.12.01

Abstract

In Vollenhovia emeryi (Hymenoptera: Myrmicinae), the queen and the male are known to be clonally reproduced. Its colonies can be classified into the two morphs with the wing length of the queen caste. The morph with normal wings is called the long-winged and the other the short-winged that is brachypterous. Even though the two morphs are considered a species, investigation on the species status of the two morphs was suggested with natural separation in nature and the distinctive wing morphology. It has yet to be determined whether the clonally reproduced queen caste is haploid or diploid. Our data clearly show that the two morphs are the same species and the queen caste is diploid on the basis of the genome size data comparison.

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Fig. 1. Two morphological types of V. emeryi queen: (a) long-winged morph (L-queen) and (b) short-winged morph (S-queen).

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Fig. 2. Flow cytometric analysis of V. emeryi two wing morphs. As the internal standard, D. melanogaster (D) was added in queen and worker samples, and queen (Q) with the corresponding wing morph with sampled male was added in the male samples. (A) Long-winged queen (B) Short-winged queen (C) Long-winged worker (D) Short-winged worker (E) Long-winged male (F) Short-winged male. Vertical axis: number of nuclei and horizontal axis: fluorescence intensity.

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Fig. 3. Amplification results of the Wolbachia specific PCR. The size of the PCR amplicons was examined with the 100 bp ladder.

Table 1. Sampling localities and geographical information of the V. emeryi colonies

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Acknowledgement

Supported by : National Research Foundation of Korea (NRF), National Institute of Ecology

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