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Mass-rearing Techniques of Anastatus orientalis (Hymenoptera: Eupelmidae), as the Egg-parasitoid of Lycorma delicatula (Hemiptera: Fulgoridae): An Using Method of Antheraea pernyi (Lepidoptera: Saturniidae) and L. delicatula Eggs in Laboratory

꽃매미 알 기생천적인 꽃매미벼룩좀벌의 대량사육기술: 산누에나방과 꽃매미 알 활용 방법

  • Seo, Meeja (Crop Protection Division, Dept. of Agro-food Safety and Crop protection, National Institute of Agricultural Sciences, RDA) ;
  • Kim, Jeong Hwan (Crop Protection Division, Dept. of Agro-food Safety and Crop protection, National Institute of Agricultural Sciences, RDA) ;
  • Seo, Bo Yoon (Crop Protection Division, Dept. of Agro-food Safety and Crop protection, National Institute of Agricultural Sciences, RDA) ;
  • Park, Changgyu (Crop Protection Division, Dept. of Agro-food Safety and Crop protection, National Institute of Agricultural Sciences, RDA) ;
  • Choi, Byeong Ryeol (Crop Protection Division, Dept. of Agro-food Safety and Crop protection, National Institute of Agricultural Sciences, RDA) ;
  • Kim, Kwang Ho (Crop Protection Division, Dept. of Agro-food Safety and Crop protection, National Institute of Agricultural Sciences, RDA) ;
  • Ji, Chang Woo (Crop Protection Division, Dept. of Agro-food Safety and Crop protection, National Institute of Agricultural Sciences, RDA) ;
  • Cho, Jum Rae (Crop Protection Division, Dept. of Agro-food Safety and Crop protection, National Institute of Agricultural Sciences, RDA)
  • 서미자 (농촌진흥청 국립농업과학원 농산물안전성부 작물보호과) ;
  • 김정환 (농촌진흥청 국립농업과학원 농산물안전성부 작물보호과) ;
  • 서보윤 (농촌진흥청 국립농업과학원 농산물안전성부 작물보호과) ;
  • 박창규 (농촌진흥청 국립농업과학원 농산물안전성부 작물보호과) ;
  • 최병렬 (농촌진흥청 국립농업과학원 농산물안전성부 작물보호과) ;
  • 김광호 (농촌진흥청 국립농업과학원 농산물안전성부 작물보호과) ;
  • 지창우 (농촌진흥청 국립농업과학원 농산물안전성부 작물보호과) ;
  • 조점래 (농촌진흥청 국립농업과학원 농산물안전성부 작물보호과)
  • Received : 2018.08.07
  • Accepted : 2018.09.15
  • Published : 2018.12.01

Abstract

Eggs, immature eggs, and pupae of 8 different insects (Halyomopha halys, Riptortus pedestris, Lymantria dispar, Antheraea yamamai, Verlarifictorus spp, Antheraea pernyi, and Musca domestica) including Lycorma delicatura were used to select the alternative host for laboratory mass rearing of A. orientalis. Except L. delicatula's eggs and immature eggs of A. pernyi, other 7 tested insects were not parasitized by A. orientalis. A. pernyi was reared with oak tree leaves and its cocoons were harvested on mid-July and early October. On 4 or 5 days after emergence, only female adults showing swollen abdomen were collected and stored at $1{\sim}5^{\circ}C$. We could get 150~200 eggs per one female by dissecting the female's abdomen. For examining the possibility for laboratory mass rearing of A. orientalis with A. pernyi's immature eggs, developmental periods from egg to pupa between the two different hosts were compared. Developmental periods were 36.1 days on immature eggs of A. pernyi and 36.8 days on an original host's eggs, respectively. The number of parasitized eggs by A. orientalis' female for 24 h was 3.4 on immature eggs of A. pernyi and 4.2 on an original host's eggs, respectively. However, there were no significant statistical differences in developmental period and parasitization between the two hosts. By supplying honeyed water to newly emerged female parasitoids, it was able to maximize their longevities up to 64.3 days after emergence. Therefore, our results support potential for laboratory mass-rearing of A. orientalis using A. pernyi's immature eggs as an alternative host.

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Fig. 1. Eggs and pupae used for selecting alternative host insects for laboratory mass rearing of A. orientalis. (A) Halyomopha halys, (B) Riptortus pedestris, (C) Lymantria dispar, (D) Antheraea yamamai, (E) Verlarifictorus spp, (F) Musca domestica, (G) Antheraea pernyi eggs, (H) Antheraea pernyi immature eggs (I) Lycorma delicatula

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Fig. 2. Mass-rearing process of Antheraea pernyi adults and immature eggs as an alternative host of L. delicatula. (A) newly emerged A. pernyi adult, (B) eggs, (C) rearing of eggs on Quercus variabilis, (D) collection of pupae on Q. variabilis, (E) selection of females after emergence, (F) storage of females at 1~5℃, (G) abdominal amputation, (H) vertical dissection of abdomen, (I) egg mass, (J) washing the egg mass on the sieve with tap water , (K) drying on the fine mesh , (L) Storage of dried eggs at 1~5℃.

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Fig. 3. Laboratory mass-rearing of A. orientalis using A. pernyi ’s immature eggs. (A) released A. orientalis adults on the alternative host eggs, (B) female wasp’s oviposition, (C) host feeding, (D) feeding and oviposition trace on the surface of host eggs, (E) emergence from the hole, (F) supply the honeyed water and host eggs as preys to the emerged adults.

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Fig. 4. Developmental periods (egg to pupa) of A. orientalis on eggs of the two insects, L. delicatula and A. pernyi. NS indicates no significant difference (P = 0.074) between the two insects.

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Fig. 5. Number of eggs oviposited by A. orientalis female within 24 hours between the two insects, L. delicatula and A. pernyi. NS indicates no significant difference (P = 0.563) between the two insects.

Table 1. Eggs and pupae used for selecting alternative host insects for laboratory mass rearing of A. orientalis

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Table 2. Longevities of A. orientalis adults on two different preys

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Acknowledgement

Supported by : 농촌진흥청

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