DOI QR코드

DOI QR Code

Susceptibility of Field Populations of Plutella xylostella and Spodoptera exigua to Four Diamide Insecticides

4종의 Diamide계열 살충제에 대한 배추좀나방과 파밤나방의 지역계통별 감수성 평가

  • Cho, Sun-Ran (Department of Plant Medicine, College of Agriculture, Life and Environment Science, Chungbuk National University) ;
  • Kyung, Yejin (Department of Plant Medicine, College of Agriculture, Life and Environment Science, Chungbuk National University) ;
  • Shin, Soeun (Department of Plant Medicine, College of Agriculture, Life and Environment Science, Chungbuk National University) ;
  • Kang, Won-Jin (Department of Plant Medicine, College of Agriculture, Life and Environment Science, Chungbuk National University) ;
  • Jung, Dae Hoon (Department of Plant Medicine, College of Agriculture, Life and Environment Science, Chungbuk National University) ;
  • Lee, Seung-Ju (Department of Plant Medicine, College of Agriculture, Life and Environment Science, Chungbuk National University) ;
  • Park, Geun-Ho (Department of Plant Medicine, College of Agriculture, Life and Environment Science, Chungbuk National University) ;
  • Kim, Sung Il (Department of Plant Medicine, College of Agriculture, Life and Environment Science, Chungbuk National University) ;
  • Cho, Sung Woo (Department of Plant Medicine, College of Agriculture, Life and Environment Science, Chungbuk National University) ;
  • Kim, Hyun Kyung (Department of Plant Medicine, College of Agriculture, Life and Environment Science, Chungbuk National University) ;
  • Koo, Hyun-Na (Department of Plant Medicine, College of Agriculture, Life and Environment Science, Chungbuk National University) ;
  • Kim, Gil-Hah (Department of Plant Medicine, College of Agriculture, Life and Environment Science, Chungbuk National University)
  • 조선란 (충북대학교 농업생명환경대학 식물의학과) ;
  • 경예진 (충북대학교 농업생명환경대학 식물의학과) ;
  • 신소은 (충북대학교 농업생명환경대학 식물의학과) ;
  • 강원진 (충북대학교 농업생명환경대학 식물의학과) ;
  • 정대훈 (충북대학교 농업생명환경대학 식물의학과) ;
  • 이승주 (충북대학교 농업생명환경대학 식물의학과) ;
  • 박근호 (충북대학교 농업생명환경대학 식물의학과) ;
  • 김성일 (충북대학교 농업생명환경대학 식물의학과) ;
  • 조성우 (충북대학교 농업생명환경대학 식물의학과) ;
  • 김현경 (충북대학교 농업생명환경대학 식물의학과) ;
  • 구현나 (충북대학교 농업생명환경대학 식물의학과) ;
  • 김길하 (충북대학교 농업생명환경대학 식물의학과)
  • Received : 2018.02.05
  • Accepted : 2018.02.21
  • Published : 2018.03.01

Abstract

The present study evaluated the susceptibility of field populations of Plutella xylostella and Spodoptera exigua larvae to four diamide insecticides-chlorantraniliprole, cyantraniliprole, cyclaniliprole, and flubendiamide. All the four diamide insecticides induced 100% mortality in the populations from Seongju (SJ) and Geochang (GC) when treated at a concentration recommended for P. xylostella. However, a very low insecticidal activity was observed in the population from Pyeongchang (PC) with 42.3% 3 d after treatment with chlorantraniliprole. The relative resistance ratio of P. xylostella to cyclaniliprole was similar or low (0.1-6.3 folds) in all the four areas. However, the population from PC exhibited a high resistance ratio to chlorantraniliprole (1,196.3 folds), cyantraniliprole (105.6 folds), and flubendiamide (191.6 folds) compared with that of the susceptible strain P. xylostella. Further, the populations of S. exigua from Cheongju (CJ), Jindo (JD), and Yeonggwang (YG) were not completely controlled by the 4 diamide insecticides. These populations also showed high relative resistance ratio when compared with that of the susceptible strain S. exigua. In particular, the populations from CJ and JD showed 100,000-fold higher resistance ratio to flubendiamide. A comparison of susceptibility of S. exigua larvae to chlorantraniliprole between 2014 and 2017 showed that chlorantraniliprole induced 100% mortality in all populations in 2014, whereas a very low insecticidal activity was observed among the populations in 2017. The results of the present study indicate that the insect pests rapidly develop resistance to diamide insecticides. Therefore, alternative insecticides with a different mechanism of action and comprehensive control strategy are needed. This study can serve as a basis to control pests effectively using diamide insecticides.

Keywords

Plutella xylostella;Spodoptera exigua;Chlorantraniliprole;Cyantraniliprole;Cyclaniliprole;Flubendiamide

References

  1. Aldosari, S.A., Watson, T.F., Sivasupramaniam, S., Osman, A.A., 1996. Susceptibility of field populations of beet armyworm (Lepidoptera: Noctuidae) to cyfluthrin, methomyl, and profenofos, and selection for resistance to cyfluthrin. J. Econ. Entomol. 89, 1359-1363.
  2. Arthrpod Pesticide Resistance Database (APRD), 2017. https://www.pesticideresistance.org/display.php?page=species&arId=571. Accessed 19 January 2018.
  3. Brewer, M.J., Trumble, J.T., 1989. Field monitoring for insecticide resistance in beet armyworm (Lepidoptera: Noctuidae). J. Econ. Entomol. 82, 1520-1526.
  4. Che, W., Shi, T., Wu, Y., Yang, Y., 2013. Insecticide resistance status of field populations of Spodoptera exigua (Lepidoptera: Noctuidae) from China. J. Econ. Entomol. 106, 1855-1862.
  5. Cho, J.M., Kim, K.J., Kim, S.M., Han, D.S., Hur, J.H., 2001. Diamondback moth (Plutella xylostella L.) resistance to organophosphorus and carbamate insecticides in Kangwon alpine vegetable croplands. Korean J. Pest. Sci. 5, 30-35.
  6. Cordova, D., Benner, E.A., Sacher, M.D., Rauh, J.J., Sopa, J.S., Lahm, G.P., Selby, T.P., Stevenson, T.M., Flexner, L., Gutteridge, S., Rhoades, D.F., Wu, L., Smith, R.M., Tao, Y., 2006. Anthranilic diamides: a new class of insecticides with a novel mode of action, ryanodine receptor activation. Pestic. Biochem. Physiol. 84, 196-214.
  7. Gong, W., Yan, H.H., Gao, L., Guo, Y.Y., Xue, C.B., 2014. Chlorantraniliprole resistance in the diamondback moth (Lepidoptera: Plutellidae). J. Econ. Entomol. 107, 806-814.
  8. Hirooka, T., Nishimatsu, T., Kodama, H., Reckmann, U., Nauen, R., 2007. The biological profile of flubendiamide, a new benzenedicarboxamide insecticide. Pflanzenschutz Nachrichten-Bayer-English Edition 60, 183.
  9. IRAC (Insecticide Resistance Action Committee), 2017. IRAC mode of action classification scheme. http://www.irac-online.org/documents/moa-classification/. Accessed 19 January 2018.
  10. Kang, W.J., Koo, H.N., Jeong, D.H., Kim, H.K., Kim, J., Kim, G.H., 2017. Functional and genetic characteristics of Chlorantraniliprole resistance in the diamondback moth, Plutella xylostella (Lepidoptera: Plutellidae). Entomol. Res. 47, 394-403.
  11. Kim, Y.R., Cho, M.S., Oh, S.M., Kim, S.W., Youn, Y.N., Yu, Y.M., 2010. Resistance and susceptibility of diamondback moth, Plutella xylostella strains collected from different region in Korea to Bacillus thuringiensis. Korean J. Pestic. Sci. 14, 123-132.
  12. Lahm, G.P., Stevenson, T.M., Selby, T.P., Freudenberger, J.H., Cordova, D., Flexner, L., Bellin. C.A., Dubas, C.M., Smith, B.K., Hughes, K.A., Hollingshaus, J.G., Clark, C.E., Benner, E.A., 2007. $Rynaxypyr^{TM}$ : a new insecticidal anthranilic diamide that acts as a potent and selective ryanodine receptor activator. Bioorg. Med. Chem. Lett. 17, 6274-6279.
  13. Lahm, G.P., Thomas, S.P., Freudenberger, J.H., Stevenson, T.M., Myers, B.J., Seburyamo, G., Smith, B.K., Flexner, L., Clark, C.E., Cordova, D., 2005. Insecticidal anthranilic diamides: a new class of potent ryanodine receptor activators. Bioorg. Med. Chem. Lett. 15, 4898-4906.
  14. Lai, T., Li, J., Su, J., 2011. Monitoring of beet armyworm Spodoptera exigua (Lepidoptera: Noctuidae) resistance to chlorantraniliprole in China. Pestic. Biochem. Physiol. 101, 198-205.
  15. Lanner, J.T., Georgiou, D.K., Joshi, A.D., Hamilton, S.L., 2010. Ryanodine receptors: structure, expression, molecular details, and function in calcium release. Cold Spring Harb Perspect Biol. 2, a003996.
  16. Lee, S.C., Cho, Y.S., Kim, D.I., 1993. Comparative study of toxicological methods and field resistance to insecticides in diamondback moth (Lepidoptera: Plutellidae). Korean J. Appl. Entomol. 32, 323-329.
  17. Metcalf, R.L., Flint, W.P., 1993. Destructive and useful insects: their habitats and control, 5th edition. McGraw-Hill Book Company, Inc., NY.
  18. Moulton, J.K., Pepper, D.A., Dennehy, T.J., 2000. Beet armyworm (Spodoptera exigua) resistance to spinosad. Pest Manag. Sci. 56, 842-848.
  19. Moulton, J.K., Pepper, D.A., Jansson, R.K., Dennehy, T.J., 2002. Pro-active management of beet armyworm (Lepidoptera: Noctuidae) resistance to tebufenozide and methoxyfenozide: baseline monitoring, risk assessment, and isolation of resistance. J. Econ. Entomol. 95, 414-424.
  20. Muthusamy, R., Vishnupriya, M., Shivakumar, M.S., 2014. Biochemical mechanism of chlorantraniliprole resistance in Spodoptera litura (Fab) (Lepidoptera: Noctuidae). J. Asia Pac. Entomol. 17, 865-869.
  21. Osorio, A., Martinez, A.M., Schneider, M.I., Diaz, O., Corrales, J.L., Aviles, M.C., Smagghe, G., Pineda, S., 2008. Monitoring of beet armyworm resistance to spinosad and methoxyfenozide in Mexico. Pest Manag. Sci. 64, 1001-1007.
  22. Pu, X., Yang, Y., Wu, S., Wu, Y., 2010. Characterisation of abamectin resistance in a field-evolved multiresistant population of Plutella xylostella. Pest Manag. Sci. 66, 371-378.
  23. Roditakis, E., Vasakis, E., Grispou, M., Stavrakaki, M., Nauen, R., Gravouil, M., Bassi, A., 2015. First report of Tuta absoluta resistance to diamide insecticides. J. Pest. Sci. 88, 9-16.
  24. Santulli, G., Marks, A.R., 2015. Essential roles of intracellular calcium release channels in muscle, brain, metabolism, and aging. Curr. Mol. Pharmacol. 8, 206-222.
  25. SAS Instituent, 2009. SAS user's guide; statistics, version 9.1 ed. SAS Institute, Cary, NC.
  26. Sattelle, D.B., Cordova, D., Cheek, T.R., 2008. Insect ryanodine receptors: molecular targets for novel pest control chemicals. Invert. Neurosci. 8, 107.
  27. Steinbach, D., Gutbrod, O., Lummen, P., Matthiesen, S., Schorn, C., Nauen, R., 2015. Geographic spread, genetics and functional characteristics of ryanodine receptor based target-site resistance to diamide insecticides in diamondback moth, Plutella xylostella. Insect Biochem. Mol. Biol. 63, 14-22.
  28. Teixeira, L.A., Andaloro, J.T., 2013. Diamide insecticides: Global efforts to address insect resistance stewardship challenges. Pestic. Biochem. Physiol. 106, 76-78.
  29. Tohnishi, M., Nakao, H., Furuya, T., Seo, A., Kodama, H., Tsubata, K., Fujioka, S., Kodama, H., Hirooka, T., Nishimatsu, T., 2005. Flubendiamide, a novel insecticide highly active against lepidopterous insect pests. J. Pest. Sci. 30, 354-360.
  30. Troczka, B., Zimmer, C.T., Elias, J., Schorn, C., Bass, C., Davies, T.G. E., Fidel, L.M., Williamson, M.S., Slater, R., Nauen, R., 2012. Resistance to diamide insecticides in diamondback moth, Plutella xylostella (Lepidoptera: Plutellidae) is associated with a mutation in the membrane-spanning domain of the ryanodine receptor. Insect Biochem. Mol. Biol. 42, 873-880.
  31. Uchiyama, T., Ozawa, A., 2014. Rapid development of resistance to diamide insecticides in the smaller tea tortrix, Adoxophyes honmai (Lepidoptera: Tortricidae), in the tea fields of Shizuoka Prefecture, Japan. Appl. Entomol. Zool. 49, 529-534.
  32. Wang, X., Khakame, S.K., Ye, C., Yang, Y., Wu, Y., 2013. Characterisation of field-evolved resistance to chlorantraniliprole in the diamondback moth, Plutella xylostella, from China. Pest Manag. Sci. 69, 661-665.
  33. Wang, X., Wu, Y., 2012. High levels of resistance to chlorantraniliprole evolved in field populations of Plutella xylostella. J. Econ. Entomol. 105, 1019-1023.
  34. Wang, X.L., Li, X.Y., Shen, A.D., Wu, Y.D., 2010. Baseline susceptibility of the diamondback moth (Lepidoptera: Plutellidae) to chlorantraniliprole in China. J. Econ. Entomol. 103, 843-848.
  35. Yao, R., Zhao, D.D., Zhang, S., Zhou, L.Q., Wang, X., Gao, C.F., Wu, S.F., 2017. Monitoring and mechanisms of insecticide resistance in Chilo suppressalis (Lepidoptera: Crambidae), with special reference to diamides. Pest Manag. Sci. 73, 1169-1178.
  36. Zalucki, M.P., Shabbir, A., Silva, R., Adamson, D., Shu-Sheng, L., Furlong, M.J., 2012. Estimating the economic cost of one of the world's major insect pests, Plutella xylostella (Lepidoptera: Plutellidae): just how long is a piece of string? J. Econ. Entomol. 105, 1115-1129.
  37. Zhang, P., Gao, M., Mu, W., Zhou, C., Li, X.H., 2014. Resistant levels of Spodoptera exigua to eight various insecticides in Shandong, China. J. Pest. Sci. 39, 7-13.
  38. Zhao, F., Wang, M., Li, J., 2006. Resistance of Plutella xylostella to nine insecticides in several field populations in China. Chinese Bull. Entomol. 43, 640-643.
  39. Zuo, Y., Wang, H., Xu, Y., Huang, J., Wu, S., Wu, Y., Yang, Y., 2017. CRISPR/Cas9 mediated G4946E substitution in the ryanodine receptor of Spodoptera exigua confers high levels of resistance to diamide insecticides. Insect Biochem. Mol. Biol. 89, 79-85.