Cellular Immune Response of Protaetia brevitarsis seulensis Larvae to Metabolites Produced by Pathogenic and Symbiotic Bacteria

흰점박이꽃무지(Protaetia brevitarsis seulensis) 유충에서 병원균과 공생균 분비물질들에 의한 세포성면역반응

  • Hwang, Dooseon (Department of Applied Biology, Division of Bioresource Sciences, College of Agriculture and Life Science, Environment Friendly Agriculture Center, Kangwon National University) ;
  • Cho, Saeyoull (Department of Applied Biology, Division of Bioresource Sciences, College of Agriculture and Life Science, Environment Friendly Agriculture Center, Kangwon National University)
  • 황두선 (강원대학교 생물자원과학부 응용생물전공) ;
  • 조세열 (강원대학교 생물자원과학부 응용생물전공)
  • Received : 2017.09.19
  • Accepted : 2018.01.30
  • Published : 2018.03.01


Studies of immune responses in insects have focused on mechanisms that interact directly with invading microorganisms. However, few studies have examined the immune response to various metabolites produced by microorganisms after they enter the host. Here, we examined immune responses in Protaetia brevitarsis seulensis larvae induced by metabolites produced by symbiotic and pathogenic bacteria. The two types of bacteria were cultured under the same conditions. The bacteria were then removed and the remaining culture supernatant was injected into the larvae. The larvae injected with culture medium (Ch-medium) from symbiotic bacteria remained relatively healthy and did not develop an immune response, whereas more than 60% of the larvae injected with pathogen culture medium (Ec-medium) died after 150 hours and dark brown patches of melanin were observed at the injection site. This immune response was confirmed by the finding of activated lysosomes in insect granulocytes. More than 50% of lysosomes in larvae injected with pathogen culture medium were strongly stained after 12 h, but less than 5% of those injected with symbiotic culture media were stained. Therefore, it is assumed that symbiotic bacteria produce few (if any) substances that induce host immune responses.


Protaetia brevitarsis seulensis;Immune;Metabolite;Granulocytes


Supported by : 한국연구재단, 강원대학교


  1. Bang, K., Hwang, S., Lee, J., Cho, S., 2015. Identification of immunity-related genes in the larvae of Protaetia brevitarsis seulensis (Coleoptera: Cetoniidae) by a next-generation sequencing-based transcriptome analysis. J. Insect Sci. 15, 142.
  2. Brestoff, J.R., Artis, D., 2013. Commensal bacteria at the interface of host metabolism and the immune system. Nat. Immunol. 14, 676-684.
  3. Buchon, N., Silverman, N., Cherry, S., 2014. Immunity in Drosophila melanogaster from microbial recognition to whole organism physiology. Nat. Rev. Immunol. 14, 796-810.
  4. Carlsson, A., Nystrom T., de Cock H., Bennich H., 1998. Attacin-an insect immune protein-binds LPS and triggers the specific inhibition of bacterial outer-membrane protein synthesis. Microbiology 144, 2179-2188.
  5. Charles, H.M., Killian K.A., 2015. Response of the insect immune system to three different immune challenges. J. Insect Physiol. 81, 97-108.
  6. Cheng, J., Wang, Y., Li, F., Liu, J., Sun, Y., Wu, J., 2014. Cloning and characterization of a mannose binding C-type lectin gene from salivary gland of Aedes albopictus. Parasites Vectors 7, 337.
  7. Cho, S., 2016. Ultrastructure characterization of hemocytes in larva of Protaetia brevitarsis seulensis. Korean J. Appl. Entomol. 55, 215-221.
  8. Ferrandon, D., Imler, J., Hetru, C., Hoffmann, J.A., 2007. The Drosophila systemic immune response: sensing and signalling during bacterial and fungal infections. Nat. Rev. Immunol. 7, 862-874.
  9. Hwang, S., Bang, K., Lee, J., Cho, S., 2015. Circulating hemocytes from larvae of the Japanese rhinoceros beetle Allomyrina dichotoma (Linnaeus) (Coleoptera: Scarabaeidae) and the cellular immune response to microorganisms. PLoS ONE. 10, e0128519.
  10. Kwon, O., 2009. Effect of different diets on larval growth of Protaetia brevitarsis seulensis (Kolbe) (Coleoptera: Cetoniidae). Entomol. Res. 39, 152-154.
  11. Kwon, H., Bang, K., Cho, S., 2014. Characterization of the Hemocytes in Larvae of Protaetia brevitarsis seulensis: Involvement of Granulocyte-Mediated Phagocytosis. PLoS ONE. 9, e103620.
  12. Lavine, M.D., Strand, M.R., 2002. Insect hemocytes and their role in immunity. Insect Biochem. Mol. Biol. 32, 1295-1309.
  13. Lee, J., Hwang, S., Cho, S., 2016. Immune tolerance to an intestineadapted bacteria, Chryseobacterium sp., injected into the hemocoel of Protaetia brevitarsis seulensis. Sci. Rep. 6, 31722.
  14. Lemaitre, B., Hoffmann J., 2007. The host defense of Drosophila melanogaster. Annu. Rev. Immunol. 25, 697-743.
  15. Lu, A., Zhang, Q., Zhang, I., Yang, B., Wu, K., Xie, W., Luan, Y., Ling, E., 2014. Insect prophenoloxidase: the view beyond immunity. Front Physiol. 5, 252.
  16. Michel, T., Reichhart, J., Hoffmann, J.A., Royet, J., 2001. Drosophila Toll is activated by Gram-positive bacteria through a circulating peptidoglycan recognition protein. Nat. 414, 756-759.
  17. Rooks, M.G., Garrett, W.S., 2016. Gut microbiota, metabolites and host immunity. Nat. Rev. Immunol. 16, 341-352.
  18. Sajed, T., Marcu, A., Ramirez, M., Pon, A., Guo, A., Knox, C., Wilson, M., Grant, J., Djoumbou, Y., Wishart, D., 2015. ECMDB 2.0: A richer resource for understanding the biochemistry of E. coli. Nucleic Acids Res. 44, D495-501.
  19. Shelby, K.S., Popham, H.J., 2012. RNA-seq study of microbially induced hemocyte transcripts from larval Heliothis virescens (Lepidoptera: Noctuidae). Insects 3, 743-762.
  20. Strand, M.R., 2008. The insect cellular immune response. Insect Sci. 15, 1-14.
  21. Vlisidou, I., Wood, W., 2015. Drosophila blood cells and their role in immune responses. FEBS J. 282, 1368-1382.