Removal of polycyclic aromatic hydrocarbons from contaminated soil in a two-phase partitioning bioreactor

  • Lee, Jae-Young (Transportation Environmental Research Team, Korea Railroad Research Institute (KRRI)) ;
  • Kwon, Tae-Soon (Railroad Safety Research Division, Korea Railroad Research Institute (KRRI)) ;
  • Lee, Young-Chul (Department of BioNano Technology, Gachon University)
  • Received : 2017.03.28
  • Accepted : 2017.05.21
  • Published : 2017.09.01


A two-phase partitioning bioreactor was employed to remediate soil contaminated by a mixture of polycyclic aromatic hydrocarbons consisting of phenanthrene, anthracene, and pyrene. In this study, the transfer of three PAHs into the water-immiscible liquid phase (silicone oil or paraffine oil) from the soil was investigated during the first 24 h. And then, phenanthrene and anthracene were degraded by approximately 90% and 80%, respectively, compared with initial concentration in soil, but pyrene was not degraded during seven days of operation period. In addition, the feasibility of a soil slurry sequencing batch reactor system in terms of continuously operating a two-phase partitioning bioreactor was investigated. Phenanthrene and anthracene were degraded semi-continuously and repeatedly during two operating cycles. Pyrene was still not degraded and was just transferred into the water-immiscible liquid phase considering its solubility.


Supported by : Korea Railroad Research Institute (KRRI), Korea Environmental Industry & Technology Institute (KEITI)


  1. S. Gan, E.V. Lau and H.K. Ng, J. Hazard. Mater., 172, 532 (2009).
  2. N. Nasirpoour, S.M. Mousavi and S. A. Shojaosadati, Korean J. Chem. Eng., 32(5), 874 (2015).
  3. M. Gatheru Waigi, F. Kang, C. Goikavi, W. Ling and Y. Gao, Int. Biodeterior. Biodegradation, 104, 333 (2015).
  4. J.-C. Yoo, C. Lee, J.-S. Lee and K. Baek, J. Environ. Manage., 186, 314 (2017).
  5. C. Roh, C. Kang and J.R. Lloyd, Korean J. Chem. Eng., 32(9), 1720 (2015).
  6. N. Gonzalez, R. Simarro, M. C. Molona, L. F. Bautista, L. Delgado and J.A. Villa, Bioresour. Technol., 102, 9438 (2011).
  7. D. Zhang, L. Zhu and F. Li, Bioresour. Technol., 142, 454 (2013).
  8. H. Ni, W. Zhou and L. Zhu, J. Environ. Sci., 26, 1071 (2014).
  9. A. J. Daugulis, Trends in Biotechol., 19(11), 457 (2001).
  10. E. Deziel, Y. Comeau and R. Villemur, Biodegradation, 10, 219 (1999).
  11. R. Villemur, E. Deziel, A. Benachenhou, J. Marcoux, E. Gauthier, J. Marcoux, E. Gauthier, F. Lepine, R. Beaudet and Y. Comeau, Biotechnol. Prog., 16(6), 966 (2000).
  12. B. Guieysse, M. d.D.T. G. Girne and B. Mattiasson, Appl. Microbiol. Biotechnol., 56, 796 (2001).
  13. R.A. Efroymson and M. Alexander, Environ. Sci. Technol., 28, 1172 (1994).
  14. T.B. Janikowski, D. Velicogna, M. Punt and A. J. Daugulis, Appl. Microbiol. Biotechnol., 59, 368 (2002).
  15. R. Munoz, B. Guieysse and B. Mattiasson, Appl. Microbiol. Biotechnol., 61, 261 (2003).
  16. M. Lu, Z. Zhang, S. Sun, Q. Wang and W. Zhong, Chemosphere, 77, 161 (2009).
  17. C. Wang, F. Wang, T. Wang, Y. Bian, X. Yang and X. Jing, J. Hazard. Mater., 176, 41 (2010).
  18. A. Arca-Ramos, G. Eined, M.T. Moreira, G. Feijoo and J.M. Lema, Chem. Eng. J., 240, 281 (2014).
  19. J.-Y. Lee and T.-S. Kwon, J. Ind. Eng. Chem., 47, 46 (2017).
  20. R. Munoz, S. Villaverde, B. Guieysse and S. Revah, Biotechnol. Adv., 25, 410 (2007).
  21. R. Munoz, A. J. Daugulis, M. Hernandez and G. Quijano, Biotechnol. Adv., 20, 1707 (2012).
  22. E.E. Poleo and A. J. Dauglis, J. Hazard. Mater., 254-255, 206 (2013).
  23. D.P. Cassidy, S. Efendiev and D. M. White, Water Res., 34(18), 4333 (2000).
  24. D. P. Cassidy and A. J. Hudak, J. Hazard. Mater., B84, 253 (2001).
  25. A. Chiavola, R. Baciocchi and R. Gavasci, J. Hazard. Mater., 184, 97 (2010).
  26. Y. Ahn, B.-G. Jung, N.-C. Sung and Y.-O. Lee, J. Life Sci., 19(5), 659 (2009).
  27. J.-Y. Lee, H.-J. Cho, K. Baek and J.-W. Yang, J. Environ. Sci. Heal. A, 40, 509 (2005).
  28. J.-W Yang, Y.-J. Lee, J.-Y. Park, S.-J. Kim and J.-Y. Lee, Eng. Geol., 77, 243 (2005).
  29. J.-Y. Park, H.-H, Lee, S.-J. Kim, Y.-J. Lee and J.-W. Yang, J. Hazard. Mater., 140, 230 (2007).