- Volume 30 Issue 12
DOI QR Code
Characterization of porcine cytokine inducible SH2-containing protein gene and its association with piglet diarrhea traits
- Niu, Buyue (College of Animal Science and Technology, Northeast Agricultural University) ;
- Guo, Dongchun (State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS)) ;
- Liu, Zhiran (College of Animal Science and Technology, Northeast Agricultural University) ;
- Han, Xiaofei (College of Animal Science and Technology, Northeast Agricultural University) ;
- Wang, Xibiao (College of Animal Science and Technology, Northeast Agricultural University)
- Received : 2016.03.01
- Accepted : 2017.05.08
- Published : 2017.12.01
Objective: The cytokine inducible SH2-containing protein (CISH), which might play a role in porcine intestine immune responses, was one of the promising candidate genes for piglet anti-disease traits. An experiment was conducted to characterize the porcine CISH (pCISH) gene and to evaluate its genetic effects on pig anti-disease breeding. Methods: Both reverse transcription polymerase chain reaction (RT-PCR) and PCR were performed to obtain the sequence of pCISH gene. A pEGFP-C1-CISH vector was constructed and transfected into PK-15 cells to analysis the distribution of pCISH. The sequences of individuals were compared with each other to find the polymorphisms in pCISH gene. The association analysis was performed in Min pigs and Landrace pigs to evaluate the genetic effects on piglet diarrhea traits. Results: In the present research, the coding sequence and genomic sequence of pCISH gene was obtained. Porcine CISH was mainly localized in cytoplasm. TaqI and HaeIII PCR restriction fragment length polymorphism (RFLP) assays were established to detect single nucleotide polymorphisms (SNPs); A-1575G in promoter region and A2497C in Intron1, respectively. Association studies indicated that SNP A-1575G was significantly associated with diarrhea index of Min piglets (p<0.05) and SNP A2497C was significantly associated with the diarrhea trait of both Min pig and Landrace piglets (p<0.05). Conclusion: This study suggested that the pCISH gene might be a novel candidate gene for pig anti-disease traits, and further studies are needed to confirm the results of this preliminary research.
Supported by : National Natural Science Foundation of China, Ministry of Education of China
- Akhtar LN, Benveniste EN. Viral exploitation of host SOCS protein functions. J Virol 2011;85:1912-21. https://doi.org/10.1128/JVI.01857-10
- Yoshimura A, Naka T, Kubo M. SOCS proteins, cytokine signaling and immune regulation. Nat Rev Immunol 2007;7:454-65. https://doi.org/10.1038/nri2093
- Yoshimura A, Ohkubo T, Kiguchi T, et al. A novel cytokine-inducible gene CIS encodes an SH2-containing protein that binds to tyrosinephosphorylated interleukin3 and erythropoietin receptors. EMBO J 1995;14:2816-26.
- Matsumoto A, Masuhara M, Mitsui K, et al. CIS, a cytokine inducible SH2 protein, is a target of the JAK-STAT5 pathway and modulates STAT5 activation. Blood 1997;89:3148-54.
- Nakajima H, Liu XW, Wynshaw-Boris A, et al. An indirect effect of Stat5a in IL-2- induced proliferation: a critical role for Stat5a in IL-2-mediated IL-2 receptor alpha chain induction. Immunity 1997;7:691-701. https://doi.org/10.1016/S1074-7613(00)80389-1
- Moriggl R, Topham DJ, Teglund S, et al. Stat5 is required for IL-2-induced cell cycle progression of peripheral T cells. Immunity 1999;10:249-59. https://doi.org/10.1016/S1074-7613(00)80025-4
- Kovanen PE, Leonard WJ. Cytokines and immunodeficiency diseases:critical roles of the gamma(c)-dependent cytokines interleukins 2, 4, 7, 9, 15, and 21, and their signaling pathways. Immunol Rev 2004;202:67-83. https://doi.org/10.1111/j.0105-2896.2004.00203.x
- Li S, Chen S, Xu X, et al. Cytokine-induced Src homology 2 protein (CIS) promotes T cell receptor-mediated proliferation and prolongs survival of activated T cells. J Exp Med 2000;191: 985-94. https://doi.org/10.1084/jem.191.6.985
- Periasamy S, Dhiman R, Barnes PF, et al. Programmed death 1 and cytokine inducible SH2-containing protein dependent expansion of regulatory T cells upon stimulation with Mycobacterium tuberculosis. J Infect Dis 2011;203:1256-63. https://doi.org/10.1093/infdis/jir011
- Miah MA, Yoon CH, Kim J, et al. CISH is induced during DC development and regulates DC-mediated CTL activation. Eur J Immunol 2012;42:58-68. https://doi.org/10.1002/eji.201141846
- Jin P, Wang E, Provenzano M, et al. Molecular signatures induced by interleukin-2 on peripheral blood mononuclear cells and T cell subsets. J Transl Med 2006;4:26. https://doi.org/10.1186/1479-5876-4-26
- Khor CC, Vannberg FO, Chapman SJ, et al. CISH and susceptibility to infectious diseases. N Engl J Med 2010;362:2092-101. https://doi.org/10.1056/NEJMoa0905606
- Sun L, Jin YQ, Shen C, et al. Genetic contribution of CISH promoter polymorphisms to susceptibility to tuberculosis in Chinese children. PLoS One 2014;9:e92020. https://doi.org/10.1371/journal.pone.0092020
- Hu Z, Yang J, Wu Y, et al. Polymorphisms in CISH gene are associated with persistent hepatitis B virus infection in Han Chinese population. Plos One 2014;9:e100826. https://doi.org/10.1371/journal.pone.0100826
- Lunney JK, Ho CS, Wysocki M, et al. Molecular genetics of the swine major histocompatibility complex, the SLA complex. Dev Comp Immunol 2009;33:362-74. https://doi.org/10.1016/j.dci.2008.07.002
- Kelly D, O'Brien JJ, McCracken KJ. Effect of creep feeding on the incidence, duration and severity of post-weaning diarrhea in pigs. Res Vet Sci 1990;49:223-8.
- Jensik PJ, Arbogast LA. Regulation of cytokine-inducible SH2-containing protein (CIS) by ubiquitination and Elongin B/C interaction. Mol Cell Endocrinol 2015;401:130-41. https://doi.org/10.1016/j.mce.2014.10.017
- Nicola NA, Greenhalgh CJ. The suppressors of cytokine signaling (SOCS) proteins: important feedback inhibitors of cytokine action. Exp Hematol 2000;28:1105-12. https://doi.org/10.1016/S0301-472X(00)00525-7
- Onteru SK, Ross JW, Rothschild MF. The role of gene discovery, QTL analyses and gene expression in reproductive traits in the pig. Soc Reprod Fertil Suppl 2009;66: 87-102.
- Delgado-Ortega M, Melo S, Meurens F. Expression of SOCS1-7 and CIS mRNA in porcine tissues. Vet Immunol Immunopathol 2011;144:493-8. https://doi.org/10.1016/j.vetimm.2011.08.002
- Van Laere AS, Nguyen M, Braunschweig M, et al. A regulatory mutation in IGF2 causes a major QTL effect on muscle growth in the pig. Nature 2003;425:832-6. https://doi.org/10.1038/nature02064
- Ji H, Ren J, Yan X, et al. The porcine MUC20 gene: molecular characterization and its association with susceptibility to enterotoxigenic Escherichia coli F4ab/ac. Mol Biol Rep 2011;38:1593-60. https://doi.org/10.1007/s11033-010-0268-y