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The relationship between odd- and branched-chain fatty acids and microbial nucleic acid bases in rumen

  • Liu, Keyuan ;
  • Hao, Xiaoyan ;
  • Li, Yang ;
  • Luo, Guobin ;
  • Zhang, Yonggen ;
  • Xin, Hangshu
  • Received : 2016.12.17
  • Accepted : 2017.05.22
  • Published : 2017.11.01

Abstract

Objective: This study aims to identify the relationship between odd- and branched-chain fatty acids (OBCFAs) and microbial nucleic acid bases in the rumen, and to establish a model to accurately predict microbial protein flow by using OBCFA. Methods: To develop the regression equations, data on the rumen contents of individual cows were obtained from 2 feeding experiments. In the first experiment, 3 rumen-fistulated dry dairy cows arranged in a $3{\times}3$ Latin square were fed diets of differing forage to concentration ratios (F:C). The second experiment consisted of 9 lactating Holstein dairy cows of similar body weights at the same stage of pregnancy. For each lactation stage, 3 cows with similar milk production were selected. The rumen contents were sampled at 4 time points of every two hours after morning feeding 6 h, and then to analyse the concentrations of OBCFA and microbial nucleic acid bases in the rumen samples. Results: The ruminal bacteria nucleic acid bases were significantly influenced by feeding diets of differing forge to concentration ratios and lactation stages of dairy cows (p<0.05). The concentrations of OBCFAs, especially odd-chain fatty acids and C15:0 isomers, strongly correlated with the microbial nucleic acid bases in the rumen (p<0.05). The equations of ruminal microbial nucleic acid bases established by ruminal OBCFAs contents showed a good predictive capacity, as indicated by reasonably low standard errors and high R-squared values. Conclusion: This finding suggests that the rumen OBCFA composition could be used as an internal marker of rumen microbial matter.

Keywords

Rumen Odd- and Branched-chain Fatty Acids;Microbial Nucleic Acid Bases;Regression Equations

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Acknowledgement

Supported by : Education Department of Heilongjiang Province