Fermentation quality and in vitro methane production of sorghum silage prepared with cellulase and lactic acid bacteria

  • Khota, Waroon (Faculty of Agriculture, Khon Kaen University) ;
  • Pholsen, Suradej (Faculty of Agriculture, Khon Kaen University) ;
  • Higgs, David (Department of Biological and Environmental Sciences, University of Hertfordshire) ;
  • Cai, Yimin (Japan International Research Center for Agricultural Science (JIRCAS))
  • Received : 2016.07.01
  • Accepted : 2017.04.27
  • Published : 2017.11.01


Objective: The effects of lactic acid bacteria (LAB) and cellulase enzyme on fermentation quality, microorganism population, chemical composition and in vitro gas production of sorghum silages were studied. Methods: Commercial inoculant Lactobacillus plantarum Chikuso 1 (CH), local selected strain Lactobacillus casei (L. casei) TH 14 and Acremonium cellulase (AC) were used as additives in sorghum silage preparation. Results: Prior to ensiling Sorghum contained $10^4LAB$ and $10^6cfu/g$ fresh matter coliform bacteria. The chemical compositions of sorghum was 26.6% dry matter (DM), 5.2% crude protein (CP), and 69.7% DM for neutral detergent fiber. At 30 days of fermentation after ensiling, the LAB counts increased to a dominant population; the coliform bacteria and molds decreased to below detectable level. All sorghum silages were good quality with a low pH (<3.5) and high lactic acid content (>66.9 g/kg DM). When silage was inoculated with TH14, the pH value was significantly (p<0.05) lower and the CP content significantly (p<0.05) higher compared to control, CH and AC-treatments. The ratio of in vitro methane production to total gas production and DM in TH 14 and TH 14+AC treatments were significantly (p<0.05) reduced compared with other treatments while in vitro dry matter digestibility and gas production did not differ among treatments. Conclusion: The results confirmed that L. casei TH14 could improve sorghum silage fermentation, inhibit protein degradation and decrease methane production.


Cellulase;Lactic Acid Bacteria;Methane Production;Sorghum Silage


Grant : The Establishment of the Sustainable and Independent Farm Household Economy in the Rural Areas of Indo-China

Supported by : Japan International Research Center for Agricultural Sciences (JIRCAS)


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