DOI QR코드

DOI QR Code

Native plants (Phellodendron amurense and Humulus japonicus) extracts act as antioxidants to support developmental competence of bovine blastocysts

  • Do, Geon-Yeop ;
  • Kim, Jin-Woo ;
  • Park, Hyo-Jin ;
  • Yoon, Seung-Bin ;
  • Park, Jae-Young ;
  • Yang, Seul-Gi ;
  • Jung, Bae Dong ;
  • Kwon, Yong-Soo ;
  • Kang, Man-Jong ;
  • Song, Bong-Seok ;
  • Kim, Sun-Uk ;
  • Chang, Kyu-Tae ;
  • Koo, Deog-Bon
  • Received : 2016.12.23
  • Accepted : 2017.02.19
  • Published : 2017.09.01

Abstract

Objective: Phellodendron amurense (P. amurense) and Humulus japonicus (H. japonicus) are closely involved in anti-oxidative response and increasing antioxidant enzymes activities. However, the effects of their extracts on development of preimplantation bovine embryos have not been investigated. Therefore, we investigated the effects of P. amurense and H. japonicus extracts on developmental competence and quality of preimplantation bovine embryos. Methods: After in vitro fertilization, bovine embryos were cultured for 7 days in Charles Rosenkrans amino acid medium supplemented with P. amurense ($0.01{\mu}g/mL$) and H. japonicus ($0.01{\mu}g/mL$). The effect of this supplementation during in vitro culture on development competence and antioxidant was investigated. Results: We observed that the blastocysts rate was significantly increased (p<0.05) in P. amurense ($28.9%{\pm}2.9%$), H. japonicus ($30.9%{\pm}1.5%$), and a mixture of P. amurense and H. japonicus ($34.8%{\pm}2.1%$) treated groups compared with the control group ($25.4%{\pm}1.6%$). We next confirmed that the intracellular levels of reactive oxygen species (ROS) were significantly decreased (p<0.01) in P. amurense and/or H. japonicus extract treated groups when compared with the control group. Our results also showed that expression of cleaved caspase-3 and apoptotic cells of blastocysts were significantly decreased (p<0.05) in bovine blastocysts derived from both P. amurense and H. japonicus extract treated embryos. Conclusion: These results suggest that proper treatment with P. amurense and H. japonicus extracts in the development of preimplantation bovine embryos improves the quality of blastocysts, which may be related to the reduction of ROS level and apoptosis.

Keywords

Bovine Embryo;Phellodendron amurense;Humulus japonicas;Reactive Oxygen Species (ROS);Antioxidants

References

  1. Zullo G, Albero G, Neglia G, et al. L-ergothioneine supplementation during culture improves quality of bovine in vitro-produced embryos. Theriogenology 2016;85:688-97. https://doi.org/10.1016/j.theriogenology.2015.10.008
  2. Boni R, Tosti E, Roviello S, Dale B. Intercellular communication in in vivo- and in vitro-produced bovine embryos. Biol Reprod 1999;61:1050-5. https://doi.org/10.1095/biolreprod61.4.1050
  3. Leibfried-Rutledge ML, Critser ES, Eyestone WH, Northey DL, First NL. Development potential of bovine oocytes matured in vitro or in vivo. Biol Reprod 1987;36:376-83. https://doi.org/10.1095/biolreprod36.2.376
  4. Rizos D, Ward F, Duffy P, Boland MP, Lonergan P. Consequences of bovine oocyte maturation, fertilization or early embryo development in vitro versus in vivo: implications for blastocyst yield and blastocyst quality. Mol Reprod Dev 2002;61:234-48. https://doi.org/10.1002/mrd.1153
  5. Lee KS, Kim EY, Jeon K, et al. 3,4-Dihydroxyflavone acts as an antioxidant and antiapoptotic agent to support bovine embryo development in vitro. J Reprod Dev 2011;57:127-34. https://doi.org/10.1262/jrd.10-029A
  6. Thannickal VJ, Fanburg BL. Reactive oxygen species in cell signaling. Am J Physiol Lung Cell Mol Physiol 2000;279:L1005-28. https://doi.org/10.1152/ajplung.2000.279.6.L1005
  7. Elmore S. Apoptosis: a review of programmed cell death. Toxicol Pathol 2007;35:495-516. https://doi.org/10.1080/01926230701320337
  8. Betts DH, King WA. Genetic regulation of embryo death and senescence. Theriogenology 2001;55:171-91. https://doi.org/10.1016/S0093-691X(00)00453-2
  9. Kumar AP, Bhaskaran S, Ganapathy M, et al. Akt/cAMP-responsive element binding protein/cyclin D1 network: a novel target for prostate cancer inhibition in transgenic adenocarcinoma of mouse prostate model mediated by Nexrutine, a Phellodendron amurense bark extract. Clin Cancer Res 2007;13:2784-94. https://doi.org/10.1158/1078-0432.CCR-06-2974
  10. Naya Y, Kotake M. The Constituents of Hops. V. The Volatile Composition of Humulus japonicus Sieb. et Zucc. Bull Chem Soc Japan 1970;43:3594-6. https://doi.org/10.1246/bcsj.43.3594
  11. Sung B, Chung JW, Bae HR, et al. Humulus japonicus extract exhibits antioxidative and anti-aging effects via modulation of the AMPKSIRT1 pathway. Exp Ther Med 2015;9:1819-26. https://doi.org/10.3892/etm.2015.2302
  12. Wang W, Li Q, Liu Y, Chen B. Ionic liquid-aqueous solution ultrasonic-assisted extraction of three kinds of alkaloids from Phellodendron amurense Rupr and optimize conditions use response surface. Ultrason Sonochem 2015;24:13-8. https://doi.org/10.1016/j.ultsonch.2014.10.009
  13. Yan H, Sun X, Sun S, et al. Anti-ultraviolet radiation effects of Coptis chinensis and Phellodendron amurense glycans by immunomodulating and inhibiting oxidative injury. Int J Biol Macromol 2011;48:720-5. https://doi.org/10.1016/j.ijbiomac.2011.02.014
  14. Dehghani-Mohammadabadi M, Salehi M, Farifteh F, et al. Melatonin modulates the expression of BCL-xl and improve the development of vitrified embryos obtained by IVF in mice. J Assist Reprod Genet 2014;31:453-61. https://doi.org/10.1007/s10815-014-0172-9
  15. Niknafs B, Mehdipour A, Mohammadi Roushandeh A. Melatonin improves development of early mouse embryos impaired by actinomycin-D and TNF-alpha. Iran J Reprod Med 2014;12:799-804.
  16. Mehaisen GM, Saeed AM, Gad A, et al. Antioxidant capacity of melatonin on preimplantation development of fresh and vitrified rabbit embryos: morphological and molecular aspects. PLoS One 2015;10:e0139814. https://doi.org/10.1371/journal.pone.0139814
  17. Rodriguez-Osorio N, Kim IJ, Wang H, Kaya A, Memili E. Melatonin increases cleavage rate of porcine preimplantation embryos in vitro. J Pineal Res 2007;43:283-8. https://doi.org/10.1111/j.1600-079X.2007.00475.x
  18. Papis K, Poleszczuk O, Wenta-Muchalska E, Modlinski JA. Melatonin effect on bovine embryo development in vitro in relation to oxygen concentration. J Pineal Res 2007;43:321-6. https://doi.org/10.1111/j.1600-079X.2007.00479.x
  19. Ishizuka B, Kuribayashi Y, Murai K, Amemiya A, Itoh MT. The effect of melatonin on in vitro fertilization and embryo development in mice. J Pineal Res 2000;28:48-51. https://doi.org/10.1034/j.1600-079x.2000.280107.x
  20. Min SH, Kim JW, Do GY, et al. Effect of Humulus japonicus extract on sperm motility, fertilization status and subsequent preimplantation embryo development in cattle. Reprod Dev Biol 2014;38:115-21. https://doi.org/10.12749/RDB.2014.38.3.115
  21. Wang F, Tian X, Zhou Y, et al. Melatonin improves the quality of in vitro produced (IVP) bovine embryos: implications for blastocyst development, cryotolerance, and modifications of relevant gene expression. PLoS One 2014;9:e93641. https://doi.org/10.1371/journal.pone.0093641
  22. Takahashi M. Oxidative stress and redox regulation on in vitro development of mammalian embryos. J Reprod Dev 2012;58:1-9. https://doi.org/10.1262/jrd.11-138N
  23. Branen AL. Toxicology and biochemistry of butylated hydroxyanisole and butylated hydroxytoluene. J Am Oil Chem Soc 1975;52:59-63. https://doi.org/10.1007/BF02901825
  24. Dennery PA. Effects of oxidative stress on embryonic development. Birth Defects Res C Embryo Today 2007;81:155-62. https://doi.org/10.1002/bdrc.20098
  25. Menezo Y, Dale B, Cohen M. DNA damage and repair in human oocytes and embryos: a review. Zygote 2010;18:357-65. https://doi.org/10.1017/S0967199410000286
  26. Wang F, Tian X, Zhang L, et al. Beneficial effects of melatonin on in vitro bovine embryonic development are mediated by melatonin receptor 1. J Pineal Res 2014;56:333-42. https://doi.org/10.1111/jpi.12126
  27. Lee E, Min S-H, Song B-S, et al. Exogenous ${\gamma}$-tocotrienol promotes preimplantation development and improves the quality of porcine embryos. Reprod Fertil Dev 2015;27:481-90. https://doi.org/10.1071/RD13167
  28. Pang YW, Sun YQ, Sun WJ, et al. Melatonin inhibits paraquat-induced cell death in bovine preimplantation embryos. J Pineal Res 2016;60:155-66. https://doi.org/10.1111/jpi.12297

Cited by

  1. Mito-TEMPO improves development competence by reducing superoxide in preimplantation porcine embryos vol.8, pp.1, 2018, https://doi.org/10.1038/s41598-018-28497-5

Acknowledgement

Supported by : Rural Development Administration, KRIBB