Native plants (Phellodendron amurense and Humulus japonicus) extracts act as antioxidants to support developmental competence of bovine blastocysts

  • Do, Geon-Yeop (Department of Biotechnology, College of Engineering, Daegu University) ;
  • Kim, Jin-Woo (Department of Biotechnology, College of Engineering, Daegu University) ;
  • Park, Hyo-Jin (Department of Biotechnology, College of Engineering, Daegu University) ;
  • Yoon, Seung-Bin (Futuristic Animal Resource and Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Park, Jae-Young (Department of Biotechnology, College of Engineering, Daegu University) ;
  • Yang, Seul-Gi (Department of Biotechnology, College of Engineering, Daegu University) ;
  • Jung, Bae Dong (College of Veterinary Medicine & Institute of Veterinary Science, Kangwon National University) ;
  • Kwon, Yong-Soo (College of Pharmacy, Kangwon National University) ;
  • Kang, Man-Jong (Department of Animal Science, College of Agriculture and Life Sciences, Chonnam National University) ;
  • Song, Bong-Seok (Futuristic Animal Resource and Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Sun-Uk (Futuristic Animal Resource and Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Chang, Kyu-Tae (Futuristic Animal Resource and Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Koo, Deog-Bon (Department of Biotechnology, College of Engineering, Daegu University)
  • Received : 2016.12.23
  • Accepted : 2017.02.19
  • Published : 2017.09.01


Objective: Phellodendron amurense (P. amurense) and Humulus japonicus (H. japonicus) are closely involved in anti-oxidative response and increasing antioxidant enzymes activities. However, the effects of their extracts on development of preimplantation bovine embryos have not been investigated. Therefore, we investigated the effects of P. amurense and H. japonicus extracts on developmental competence and quality of preimplantation bovine embryos. Methods: After in vitro fertilization, bovine embryos were cultured for 7 days in Charles Rosenkrans amino acid medium supplemented with P. amurense ($0.01{\mu}g/mL$) and H. japonicus ($0.01{\mu}g/mL$). The effect of this supplementation during in vitro culture on development competence and antioxidant was investigated. Results: We observed that the blastocysts rate was significantly increased (p<0.05) in P. amurense ($28.9%{\pm}2.9%$), H. japonicus ($30.9%{\pm}1.5%$), and a mixture of P. amurense and H. japonicus ($34.8%{\pm}2.1%$) treated groups compared with the control group ($25.4%{\pm}1.6%$). We next confirmed that the intracellular levels of reactive oxygen species (ROS) were significantly decreased (p<0.01) in P. amurense and/or H. japonicus extract treated groups when compared with the control group. Our results also showed that expression of cleaved caspase-3 and apoptotic cells of blastocysts were significantly decreased (p<0.05) in bovine blastocysts derived from both P. amurense and H. japonicus extract treated embryos. Conclusion: These results suggest that proper treatment with P. amurense and H. japonicus extracts in the development of preimplantation bovine embryos improves the quality of blastocysts, which may be related to the reduction of ROS level and apoptosis.


Bovine Embryo;Phellodendron amurense;Humulus japonicas;Reactive Oxygen Species (ROS);Antioxidants


Supported by : Rural Development Administration, KRIBB


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