The Protective Effects of Dangguibohyul-tang (Dangguibuxuetang) against Disuse Muscle Atrophy in Rats

흰쥐의 불용성 근위축에 당귀보혈탕이 미치는 영향과 그 기전에 관한 고찰

  • Kim, Bum Hoi (Department of Anatomy, College of Korean Medicine and Research Institute of Oriental Medicine, Dong-eui University)
  • 김범회 (동의대학교 한의과대학 해부학교실, 동의대학교 한의학연구소)
  • Received : 2017.09.14
  • Accepted : 2017.09.29
  • Published : 2017.10.31


Objectives Oxidative stress, in which antioxidant proteins and scavenger protection are overwhelmed by reactive oxygen species (ROS) production, is recognized as one of central causes of disuse muscle atrophy. In this study, the hypothesis that oral treatment with Dangguibohyul-tang (Dangguibuxuetang) could attenuate immobilization-induced skeletal muscle atrophy was tested. Methods The hindlimb immobilization was performed with casting tape to keep the left ankle joint in a fully extended position. The Rats in Dangguibohyul-tang treated group (DGBHT) (n=10) were orally administrated Dangguibohyul-tang water extract, and rats of Control group (n=10) were given with saline only. After 2 weeks of immobilization, the morphology of right and left gastrocnemius muscles in both DGBHT and Control groups were assessed by hematoxylin and eosin staining. Results Dangguibohyul-tang water extract represented the significant protective effects against the reductions of the left gastrocnemius muscles weight and average cross section area to compared with Control group. Moreover, the treatment with Dangguibohyul-tang extract significantly enhanced the Cu/Zn-SOD activities in gastrocnemius muscle compared with Control group. Conclusions Thses results suggest that Dangguibohyul-tang has protective effects against immobilization-induced muscle atrophy by increasing the Cu/Zn-SOD activities in gastrocnemius muscle.


Disuse muscle atrophy;Dangguibohyul-tang;Gastrocnemius;Reactive oxygen species (ROS);Superoxide dismutase (SOD)


Supported by : 동의대학교


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