- Volume 33 Issue 2
DOI QR Code
Characterization of Pyrenophora tritici-repentis (Tan Spot of Wheat) Races in Baltic States and Romania
- Abdullah, Sidrat (Department of Agronomy, Horticulture and Plant Science, South Dakota State University) ;
- Sehgal, Sunish Kumar (Department of Agronomy, Horticulture and Plant Science, South Dakota State University) ;
- Ali, Shaukat (Department of Agronomy, Horticulture and Plant Science, South Dakota State University) ;
- Liatukas, Zilvinas (Institute of Agriculture, Lithuanian Research Center for Agriculture and Forestry) ;
- Ittu, Mariana (National Agricultural Research and Development Institute Fundulea) ;
- Kaur, Navjot (Department of Agronomy, Horticulture and Plant Science, South Dakota State University)
- Received : 2016.10.10
- Accepted : 2016.12.11
- Published : 2017.04.01
Tan spot, caused by the fungus Pyrenophora tritici-repentis, is economically important foliar disease in Latvia, Lithuania, and Romania; however, race structure from Baltic States and Romania is not known. In this study, we performed genotypic and phenotypic race characterization of a large collection of P. tritici-repentis isolates from these countries to determine race structure and utilize this information for better disease management and breeding wheat for tan spot resistance. We characterized 231 single spore isolates from Latvia (n = 15), Lithuania (n = 107), and Romania (n = 109) for Ptr ToxA and Ptr ToxB genes using two genes specific primers. A subset (139) of 231 isolates were further characterized for their race structure by inoculating them individually on tan spot wheat differentials set. Majority (83%) of the 231 isolates amplified Ptr ToxA gene suggesting prevalence of race 1 and 2. Further, phenotypic characterization of 139 isolates also showed wide prevalence of races 1 (68%), 2 (8%), 3 (11%), and 4 (5%) were also identified from Baltic States as well as Romania. Eighteen of the isolates (13%) did not seem to be of any of the eight known races as they lacked Ptr ToxA gene but they behaved like either race 1 or race 2, suggesting possibility of novel toxins in these isolates as their virulence tools.
Supported by : South Dakota Wheat Commission, SDSU Agricultural Experiment Station (AES)
- Aboudkhaddour, R., Turkington, T. K. and Strelkov, S. E. 2013. Race structure of Pyrenophora triciti-repentis (tan spot of wheat) in Alberta, Canada. Can. J. Plant Pathol. 35:256-268. https://doi.org/10.1080/07060661.2013.782470
- Ali, S. and Francl, L. J. 2001. Recovery of Pyrenophora triticirepentis from barley and reaction of 12 cultivars to five races and two host-specific toxins. Plant Dis. 85:580-584. https://doi.org/10.1094/PDIS.2001.85.6.580
- Ali, S. and Francl, L. J. 2002. Race structure of Pyrenophora tritici-repentis isolates obtained from wheat in South America. Plant Protect. Sci. 38(Special Issue 2):302-304.
- Ali, S. and Francl, L. J. 2003. Population race structure of Pyrenophora tritici-repentis prevalent on wheat and noncereal grasses in the Great Plains. Plant Dis. 87:418-422. https://doi.org/10.1094/PDIS.2003.87.4.418
- Ali, S., Gurung, S. and Adhikari, T. B. 2010. Identification and characterization of novel isolates of Pyrenophora triticirepentis from Arkansas. Plant Dis. 94:229-235. https://doi.org/10.1094/PDIS-94-2-0229
- Ali, S., Sarova, J. and Waukulinsky, W. 2004. Pyrenophora tritici-repentis races in the Czech Republic and Poland. Phytopathology 93(Suppl):S3 (Abstract).
- Ali, S., Singh, P. K., McMullen, M. P., Mergoum, M. and Adhikari, T. B. 2008. Resistance to multiple leaf spot diseases in wheat. Euphytica 159:167-179.
- Andrie, R. M., Pandelova, I. and Ciuffetti, L. M. 2007. A combination of phenotypic and genotypic characterization strengthens Pyrenophora tritici-repentis race identification. Phytopathology 97:694-701. https://doi.org/10.1094/PHYTO-97-6-0694
- Bankina, B. and Priekule, I. 2011. A review of tan spot research in Baltic countries: occurrence, biology and possibilities of control. Zemdirbyste (Agriculture) 98:3-10.
- Benslimane, H., Lamari, L., Benbelkacem, A., Sayoud, R. and Bouznad, Z. 2011. Distribution of races of Pyrenophora tritici-repentis in Algeria and identification of a new virulence type. Phytopathol. Mediterr. 50:203-211.
- Ciuffetti, L. M. and Tuori, R. P. 1999. Advances in the characterization of the Pyrenophora tritici-repentis: wheat interaction. Phytopathology 89:444-449. https://doi.org/10.1094/PHYTO.19184.108.40.2064
- da Luz, W. C. and Hosford, R. M., Jr. 1980. Twelve Pyrenophora trichostoma races for virulence to wheat in the central plains of North America. Phytopathology 70:1193-1196. https://doi.org/10.1094/Phyto-70-1193
- De Wolf, E. D., Effertz, R. J., Ali, S. and Francl, L. J. 1998. Vistas of tan spot research. Can. J. Plant Pathol. 20:349-370. https://doi.org/10.1080/07060669809500404
- Effertz, R. J., Meinhardt, S. W., Anderson, J. A., Jordahl, J. G. and Francl, L. J. 2002. Identification of a chlorosis-inducing toxin from Pyrenophora tritici-repentis and the chromosomal location of an insensitivity locus in wheat. Phytopathology 92:527-533. https://doi.org/10.1094/PHYTO.2002.92.5.527
- Engle, J. S., Madden, L. V. and Lipps, P. E. 2006. Distribution and pathogenic characterization of Pyrenophora tritici-repentis and Stagonospora nodorum in Ohio. Phytopathology 96:1355-1362. https://doi.org/10.1094/PHYTO-96-1355
- Francl, L. J. 1997. Local and mesoscale dispersal of Pyrenophora tritici-repentis conidia. Can. J. Plant Pathol. 19:247-255. https://doi.org/10.1080/07060669709500519
- Friesen, T. L., Ali, S., Kianian, S., Francl, L. J. and Rasmussen, J. B. 2003. Role of host sensitivity to Ptr ToxA in development of tan spot of wheat. Phytopathology 93:397-401. https://doi.org/10.1094/PHYTO.2003.93.4.397
- Gamba, F. M., Strelkov, S. E. and Lamari, L. 2012. Virulence of Pyrenophora tritici-repentis in the southern cone region of South America. Can. J. Plant Pathol. 34:545-550. https://doi.org/10.1080/07060661.2012.695750
- Hosford, R. M., Jr. 1982. Tan spot: developing knowledge 1902-1981, virulent races and wheat differentials, methodology, rating systems, other leaf diseases, literature. In: Tan spot of wheat and related disease workshop, ed. by R. M. Hosford, Jr., pp. 1-20. North Dakota State University, Fargo, ND, USA.
- Jordahl, J. G. and Francl, L. J. 1992. Increase and storage of cultures of Pyrenophora tritici-repentis. In: Advances in tan spot research, eds. by L. J. Francl, J. M. Krupinsky and M. P. McMullen, pp. 109. North Dakota State University, Fargo, ND, USA.
- Krupinsky, J. M. 1992. Grass hosts of Pyrenophora triticirepentis. Plant Dis. 76:92-95. https://doi.org/10.1094/PD-76-0092
- Lamari, L. and Bernier, C. C. 1989. Evaluation of wheat lines and cultivars to tan spot (Pyrenophora tritici-repentis) based on lesion type. Can. J. Plant Pathol. 11:49-56. https://doi.org/10.1080/07060668909501146
- Lamari, L., Sayoud, R., Boulif, M. and Bernier, C. C. 1995. Identification a new race in Pyrenophora tritici-repentis: implications for the current pathotype classification system. Can. J. Plant Pathol. 17:312-318. https://doi.org/10.1080/07060669509500668
- Lamari, L., Strelkov, S. E., Yahyaoui, A., Orabi, J. and Smith, R. B. 2003. The identification of two new races of Pyrenophora tritici-repentis from the host center of diversity confirms a one-to-one relationship in tan spot of wheat. Phytopathology 93:391-396. https://doi.org/10.1094/PHYTO.2003.93.4.391
- Lepoint, P., Renard, M. E., Legreve, A., Duveiller, E. and Maraite, H. 2010. Genetic diversity of the mating type and toxin production genes in Pyrenophora tritici-repentis. Phytopathology 100:474-483. https://doi.org/10.1094/PHYTO-100-5-0474
- Liatukas, Z., Ruzgas, V., Razbadauskienė, K., Brazauskas, G. and Koppel, R. 2012. Winter wheat cultivars 'Kovas DS', 'Zunda DS', 'Vikaras DS', 'Kaskada DS' for high input farming: development and characterization. Zemdirbyste (Agriculture) 99:255-264.
- Mikhalova, L. A., Mironenko, N. V. and Kovalenko, N. M. 2014. Populations of Pyrenophora tritici-repentis in the North Caucasus and North-West Russia: the racial composition and dynamics of virulence. Mikol. Fitopatol. 48:393-400.
- Mironenko, N. V., Baranova, O. A., Kovalenko, N. M. and Mikhalova, L. A. 2015. Frequency of ToxA gene in North Caucasian and North-West Russian populations of Pyrenophora tritici-repentis. Mikol. Fitopatol. 49:325-329.
- Misra, A. P. and Singh, R. A. 1972. Pathogenic differences amongst three isolates of Helminthosporium tritici-repentis and the performance of wheat varieties against them. Indian Phytopathol. 25:350-353.
- Moreno, M. V., Stenglein, S. A., Balatti, P. A. and Perello, A. E. 2008. Pathogenic and molecular variability among isolates of Pyrenophora tritici-repentis, causal agent of tan spot of wheat in Argentina. Eur. J. Plant Pathol. 122:239-252. https://doi.org/10.1007/s10658-008-9277-2
- Noriel, A. J., Xiaochun, S., Willium, B. and Guihua, B. 2011. Resistance to tan spot and insensitivity to Ptr ToxA in wheat. Crop Sci. 51:1059-1067. https://doi.org/10.2135/cropsci2010.08.0464
- Oliver, R. P., Lord, M., Rybak, K., Faris, J. D. and Solomon, P. S. 2008. Emergence of tan spot disease caused by toxigenic Pyrenophora tritici-repentis in Australia is not associated with increased deployment of toxin-sensitive cultivars. Phytopathology 98:488-491. https://doi.org/10.1094/PHYTO-98-5-0488
- Ronis, A., Semaskiene, R., Dabkevicius, Z. and Liatukas, Z. 2009. Influence of leaf diseases on grain yield and yield components in winter wheat. J. Plant Prot. Res. 49:151-157.
- Sarova, J., Hanzalova, A. and Bartos, P. 2005. Races of Pyrenophora tritici-repentis in Czech Republic. Acta Agrobot. 58:73-78.
- Schilder, A. M. C. and Bergstrom, G. C. 1990. Variation in virulence within the population of Pyrenophora tritici-repentis in New York. Phytopathology 80:84-90. https://doi.org/10.1094/Phyto-80-84
- Shabeer, A. and Bockus, W. W. 1988. Tan spot effects on yield and yield components relative to growth stage in winter wheat. Plant Dis. 72:599-602. https://doi.org/10.1094/PD-72-0599
- Sierotzki, H., Frey, R., Wullschleger, J., Palermo, S., Karlin, S., Godwin, J. and Gisi, U. 2007. Cytochrome b gene sequence and structure of Pyrenophora teres and P. tritici-repentis and implications for QoI resistance. Pest Manag. Sci. 63:225-233. https://doi.org/10.1002/ps.1330
- Singh, P. K. and Hughes, G. R. 2006. Genetic similarity among isolates of Pyrenophora tritici-repentis, causal agent of tan spot of wheat. J. Phytopathol. 154:178-184. https://doi.org/10.1111/j.1439-0434.2006.01083.x
- Singh, P. K., Mergoum, M., Ali, S., Adhikari, T. B., Elias, E. M., Anderson, J. A., Glover, K. D. and Berzonsky, W. A. 2006. Evaluation of elite wheat germ plasm for resistance to tan spot. Plant Dis. 90:1320-1325. https://doi.org/10.1094/PD-90-1320
- [USDA FAS] USDA Foreign Agricultural Service (2016 onwards). Gain report. URL http://gain.fas.usda.gov/Recent%20GAIN%20Publications/Grain%20and%20Feed%20Annual%20-%20Lithuania%202016_Warsaw_Lithuania_4-14-2016.pdf [4 January 2016].
- Characterizing Virulence of the Pyrenophora tritici-repentis Isolates Lacking Both ToxA and ToxB Genes vol.7, pp.3, 2018, https://doi.org/10.3390/pathogens7030074
- Metagenome Profiling Identifies Potential Biocontrol Agents for Selaginella kraussiana in New Zealand vol.10, pp.2, 2019, https://doi.org/10.3390/genes10020106