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Characterization of the rcsA Gene from Pantoea sp. Strain PPE7 and Its Influence on Extracellular Polysaccharide Production and Virulence on Pleurotus eryngii

Kim, Min Keun;Lee, Sun Mi;Seuk, Su Won;Ryu, Jae San;Kim, Hee Dae;Kwon, Jin Hyeuk;Choi, Yong Jo;Yun, Han Dae

  • Received : 2016.08.30
  • Accepted : 2017.02.27
  • Published : 2017.06.01

Abstract

RcsA is a positive activator of extracellular polysaccharide (EPS) synthesis in the Enterobacteriaceae. The rcsA gene of the soft rot pathogen Pantoea sp. strain PPE7 in Pleurotus eryngii was cloned by PCR amplification, and its role in EPS synthesis and virulence was investigated. The RcsA protein contains 3 highly conserved domains, and the C-terminal end of the open reading frame shared significant amino acid homology to the helix-turn-helix DNA binding motif of bacterial activator proteins. The inactivation of rcsA by insertional mutagenesis created mutants that had decreased production of EPS compared to the wild-type strain and abolished the virulence of Pantoea sp. strain PPE7 in P. eryngii. The Pantoea sp. strain PPE7 rcsA gene was shown to strongly affect the formation of the disease symptoms of a mushroom pathogen and to act as the virulence factor to cause soft rot disease in P. eryngii.

Keywords

bacterial pathogen;king oyster mushroom;Pleurotus eryngii;rcsA gene;soft rot disease

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Acknowledgement

Grant : Research Program for Agriculture Science and Technology Development

Supported by : Gyeongsangnam-do Agricultural Research & Extension Services