Comparison of silkworm powder from 3 Bombyx mori varieties on alcohol metabolism in rats

  • Lee, Da-Young ;
  • Cho, Jae-Min ;
  • Yun, Sun-Mi ;
  • Hong, Kyung-Sook ;
  • Ji, Sang-Deok ;
  • Son, Jong-Gon ;
  • Kim, Eun-Hee
  • Received : 2017.06.20
  • Accepted : 2017.08.08
  • Published : 2017.09.30


Increased alcohol consumption is a burden on the world because it is associated with various health problems. However, the effects of silkworms on alcohol metabolism have not been studied yet. The hard-to-eat mature silkworms have become easier to ingest recently due to the development of technology, steam-lyophilising mature silkworm larvae. In this study, we investigated and compared the effects of SMSPs from three silkworm varieties, Baekokjam, Golden-silk and Yeonnokjam weaving white, golden, and light green cocoons on alcohol metabolism in vivo. Sprague-Dawley rats pretreated with three SMSPs (0.1 g/kg or 1 g/kg body weight) or normal diet (AIN-76A) for 2 weeks were subjected to intragastric administration of absolute ethanol (3 g/kg body weight, 3 h). Three SMSPs did not affect the final body weight and liver weight. All 3 SMSPs were effective to reduce the enzymes in alcohol metabolism, alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH), and liver damage and enzymes involved in liver damage, aspartate aminotransferase (AST) and alanine aminotransferase (ALT). Among SMSP from 3 varieties of silkworm, preadministration of 1 g/kg Baekokjam SMSP showed the most effective suppressive effect on the activities of ADH, ALDH, AST and ALT. The Baekokjam SMSP contained higher amounts of beneficial amino acids than Golden-silk or Yeonnokjam SMSP. These results suggest that Baekokjam SMSP might be used as a new and promising candidate for improving alcohol metabolism and liver injury through promoting rapid alcohol metabolism.


Steamed and freeze-dried mature silkworm larval powder;Silkworm varieties;Ethanol;Alcohol dehydrogenase;Aldehyde dehydrogenase;SD rat


  1. Agarwal DP, Goedde HW (1989) Human aldehyde dehydrogenases: Their role in alcoholism. Alcohol Vol. 6, 517-523.
  2. Cederbaum AI (2012) Alcohol metabolism. Clin Liver Dis 16, 667-685.
  3. Cequera A, Garcia de Leon Mendez MC (2014) Biomarkers for liver fibrosis: Advances, advantages and disadvantages. Revista de Gastroenterologia de Mexico (English Edition) 79, 187-199.
  4. Cho JM, Kim KY, Ji SD, Kim EH (2016) Protective effect of boiled and freeze-dried mature silkworm larval powder against diethylnitrosamine-induced hepatotoxicity in mice. J Cancer Prev 21, 173-181.
  5. Do SG, Park JH, Nam H, Kim JB, Lee JY, Oh YS, et al. (2012) Silk fibroin hydrolysate exerts an anti-diabetic effect by increasing pancreatic ${\beta}$ cell mass in C57BL/KsJ-db/db mice. J Vet Sci 13, 339.
  6. Ji SD, Kim NS, Kweon HY, Choi BH, Kim KY, Koh YH (2016) Nutrition composition differences among steamed and freeze-dried mature silkworm larval powders made from 3 Bombyx mori varieties weaving different colored cocoons. Int J Indust Entomol 33, 6-14.
  7. Ji SD, Kim NS, Kweon HY, Choi BH, Yoon SM, Kim KY, et al. (2016) Nutrient compositions of Bombyx mori mature silkworm larval powders suggest their possible health improvement effects in humans. J Asia-Pac Entomol 19, 1027-1033.
  8. Ji SD, Kim NS, Kwon HY, Choi BH, Kim KY, Koh YH (2016) Nutrition composition differences among steamed and freeze-dried mature silkworm larval powders made from 3 Bombyx mori varieties weaving different colored cocoons. Int J Indust Entomol 33, 6-14.
  9. Ji SD, Kim NS, Lee JY, Kim MJ, Kweon HY, Sung GB, et al. (2015) Development of processing technology for edible mature silkworm. J Seri Entomol Sci 53, 38-43.
  10. Jung S, Lee SH, Song YS, Lee SY, Kim SY, Ko KS (2016) Effect of beverage containing fermented Akebia quinata extracts on alcoholic hangover. Prev Nutr Food Sci 21, 9-13.
  11. Kang PD, Lee SU, Jung IY, Shon BH, Kim YS, Kim KY, et al. (2007) Breeding of new silkworm variety golden silk, a yellow cocoon color for spring rearing season. J Seri Entomol Sci 49, 14-17.
  12. Kim YS, Kim KY, Kang PD, Cha JY, Heo JSH, Park BK, et al. (2008) Effect of silkworm (Bombyx mori) excrement powder on the alcoholic hepatotoxicity in Rats. J Life Sci 18, 1342-1347.
  13. Kunz RI, Brancalhao RM, Ribeiro LF, Natali MR (2016) Silkworm sericin: Properties and biomedical applications. Biomed Res Int 2016, 8175701.
  14. Lee S, Hong K, Sohn K, Mah Y, Kim K (1984) Breeding of new silkworm variety "BaegOkJam". Res Rept RDA 26, 58-64.
  15. Louvet A, Mathurin P (2015) Alcoholic liver disease: mechanisms of injury and targeted treatment. Nat Rev Gastroenterol Hepatol 12, 231-242.
  16. Mathews S, Xu M, Wang H, Bertola A, Gao B (2014) Animals models of gastrointestinal and liver diseases. Animal models of alcoholinduced liver disease: pathophysiology, translational relevance, and challenges. Am J Physiol Gastrointest Liver Physiol 306, G819-823.
  17. Mattia CJ, Adams JD, Jr., Bondy SC (1993) Free radical induction in the brain and liver by products of toluene catabolism. Biochem Pharmacol 46, 103-110.
  18. Park JE, Lee M, Mifflin R, Lee YK (2016) Enhanced ethanol catabolism in orphan nuclear receptor SHP-null mice. Am J Physiol Gastrointest Liver Physiol 310, G799-807.
  19. Park JH, Kim Y, Kim SH (2012) Green tea extract (Camellia sinensis) fermented by Lactobacillus fermentum attenuates alcohol-induced liver damage. Biosci Biotechnol Biochem 76, 2294-2300.
  20. Ryu KS, Lee HS, Kim KY, Kim MJ, Sung GB, Ji SD, et al. (2013) 1-Deoxynojirimycin content and blood glucose-lowering effect of silkworm (Bombyx mori) extract powder. Int J Indust Entomol 27, 237-242.
  21. Scoccianti C, Lauby-Secretan B, Bello PY, Chajes V, Romieu I (2014) Female breast cancer and alcohol consumption: a review of the literature. Am J Prev Med 46, S16-25.
  22. Seo JY, Kim SS, Kim JS (2014) Enhancement of Alcohol Metabolism by Sprouted Peanut Extract in SD Rats. Prev Nutr Food Sci 19, 1-4.
  23. Shield KD, Rylett M, Gmel G, Kehoe-Chan TA, Rehm J (2013) Global alcohol exposure estimates by country, territory and region for 2005-a contribution to the Comparative Risk Assessment for the 2010 Global Burden of Disease Study. Addiction 108, 912-922.
  24. Sung CK, Kim SM, Oh CJ, Yang SA, Han BH, Mo EK (2012) Taraxerone enhances alcohol oxidation via increases of alcohol dehyderogenase (ADH) and acetaldehyde dehydrogenase (ALDH) activities and gene expressions. Food Chem Toxicol 50, 2508-2514.
  25. Swift R, Davidson D (1998) Alcohol hangover-mechanisms and mediators. Alcohol Health Res World 22, 54-60.
  26. Tahir M, Sultana S (2011) Chrysin modulates ethanol metabolism in Wistar rats: a promising role against organ toxicities. Alcohol Alcohol 46, 383-392.


Grant : Elucidation the health improvement effects of boiled silk worm larvae

Supported by : Rural Development Administration