- Volume 35 Issue 1
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Comparative effect of silkworm powder from 3 Bombyx mori varieties on ethanol-induced gastric injury in rat model
- Lee, Da-Young (College of Pharmacy and Institute of Pharmaceutical Sciences, CHA University) ;
- Cho, Jae-Min (College of Pharmacy and Institute of Pharmaceutical Sciences, CHA University) ;
- Yun, Sun-Mi (College of Pharmacy and Institute of Pharmaceutical Sciences, CHA University) ;
- Hong, Kyung-Sook (College of Pharmacy and Institute of Pharmaceutical Sciences, CHA University) ;
- Ji, Sang-Deok (Department of Agricultural Biology, National Institute of Agricultural Science, Rural Development Administration) ;
- Son, Jong-Gon (Department of Agricultural Biology, National Institute of Agricultural Science, Rural Development Administration) ;
- Kim, Eun-Hee (College of Pharmacy and Institute of Pharmaceutical Sciences, CHA University)
- Received : 2017.07.03
- Accepted : 2017.08.08
- Published : 2017.09.30
Gastric ulcer is a clinical symptom characterized by inflammation of the gastric mucosa. Stress and alcohol consumption have been identified as the major cause of gastric ulcer. However, the effects of silkworms on ethanol-induced gastric ulcer have not been studied yet. The mature silkworms that are difficult to eat have become easier to ingest due to recent technological development to make steaming and freeze-drying mature silkworm larval powder (SMSP). In this study, we investigated whether three silkworm varieties, Baekokjam, Golden-silk and Yeonnokjam could alleviate ethanol-induced gastric mucosal damage in vivo. Sprague-Dawley rats pretreated with 3 SMSPs (0.1 or 1 g/kg BW) or normal diet (AIN-76A) were exposed to absolute ethanol (3 g/kg BW, 3 h) by oral gavage. Morphological examination included ulcer index as a measurement of hemorrhages and hematoxylin and eosin staining was performed to analyze the severity of gastric ulcer. Results of macroscopic examination suggested that all 3 SMSPs pretreatment significantly protected gastric mucosa against ethanol-induced damage. Microscopic observations demonstrated significant mucosal erosion and inflammation in ethanol-treated rats, which was abrogated in rats pretreated with 3 SMSPs. In addition, pretreatment with all 3 SMSPs showed significant decreases the expression of pro-inflammatory mediators, IL-6 and cyclooxygenase-2. Among SMSP from 3 varieties of silkworm, preadministration of 1 g/kg Baekokjam SMSP showed the most effective protective effect against ethanol-induced gastric ulcer. These results suggest that Baekokjam SMSP can be a potential gastroprotective agent against ethanol-induced gastric ulcer.
Steamed and freeze-dried mature silkworm larval powder;Silkworm varieties;Ethanol;Gastric ulcer;SD rat;Anti-inflammation
Grant : Elucidation the health improvement effects of boiled silk worm larvae
Supported by : Rural Development Administration
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