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Simultaneous Determination and Monitoring of Bisphenols in River Water using Gas Chromatography-Mass Spectrometry

GC-MS 를 이용한 하천수 중 Bisphenol계 화합물의 동시분석 및 모니터링

  • Kim, Jihyun (Yeongsan River Environmental Research Center, National Institute of Environmental Research) ;
  • Choi, Jeong-Heui (Natural Environment Research Division, National Institute of Environmental Research) ;
  • Kang, Tae-Woo (Yeongsan River Environmental Research Center, National Institute of Environmental Research) ;
  • Kang, Taegu (Yeongsan River Environmental Research Center, National Institute of Environmental Research) ;
  • Hwang, Soon-Hong (Yeongsan River Environmental Research Center, National Institute of Environmental Research) ;
  • Shim, Jae-Han (Division of Applied Bioscience and Biotechnology, College of Agriculture and Life Sciences, Chonnam National University)
  • 김지현 (국립환경과학원 영산강물환경연구소) ;
  • 최정희 (국립환경과학원 자연환경연구과) ;
  • 강태우 (국립환경과학원 영산강물환경연구소) ;
  • 강태구 (국립환경과학원 영산강물환경연구소) ;
  • 황순홍 (국립환경과학원 영산강물환경연구소) ;
  • 심재한 (전남대학교 농업생명과학대학 농식품생명화학부)
  • Received : 2017.07.05
  • Accepted : 2017.09.21
  • Published : 2017.09.30

Abstract

BACKGROUND:This study was carried out to establish an efficient sample preparation for the simultaneous determination of bisphenols (BPs) in river water samples using gas chromatography-mass spectrometry (GC-MS). Sample preparation was examined with conventional extraction methods, such as solid-phase extraction (SPE) and liquid-liquid extraction (LLE), and their efficiency was compared with validation results, including linearity of calibration curve, method detection limit (MDL), limit of quantification (LOQ), accuracy, and precision. METHODS AND RESULTS:The BPs (bisphenol A, BPA; bisphenol B, BPB; bisphenol C, BPC; bisphenol E, BPE; bisphenol F, BPF; bisphenol S, BPS) were analyzed using GC-MS. The range of MDLs by SPE and LLE methods was $0.0005{\sim}0.0234{\mu}g/L$ and $0.0037{\sim}0.2034{\mu}g/L$, and that of LOQs was $0.0015{\sim}0.0744{\mu}g/L$ and $0.0117{\sim}0.6477{\mu}g/L$, respectively. The calibration curve obtained from standard solution of $0.004{\sim}4.0{\mu}g/L$ (SPE) and $0.016{\sim}16{\mu}g/L$ (LLE) showed good linearity with $r^2$ value of 0.9969 over. Accuracy was 93.2~108% and 97.4~120%, and precision was 1.7~4.6% and 0.7~6.5%, respectively. The values of MDL and LOQ resulted from the SPE method were higher than those from the LLE method, particularly those values of BPA were highest among the BPs. Based on the results, the SPE method was applied to determine the BPs in river water samples. Water samples were collected from mainstream, tributary and sewage wastewater treatment plants (SWTPs) in the Yeongsan river basin. The concentration of BPB, BPC, BPE, BPF and BPS were not detected in all sites, whereas BPA was ranged $0.0095{\sim}0.2583{\mu}g/L$, which was $0.0166{\sim}0.0810{\mu}g/L$ for mainstreams, $0.0095{\sim}0.2583{\mu}g/L$ for tributaries, $0.0352{\sim}0.1217{\mu}g/L$ for SWTPs. CONCLUSION: From these results, the SPE method was very effective for the simultaneous determination of BPs in river water samples using GC-MS. We provided that it is a convenient, reliable and sensitive method enough to monitor and understand the fate of the BPs in aquatic ecosystems.

Keywords

BPA;GC-MS;Liquid-liquid extraction;Solid-phase extraction;Yeongsan River

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