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Rapid Detection for Lysinibacillus fusiformis, a Suspicious Pathogen of Bombus terrestris, using Ultra-Rapid PCR

초고속 유전자 증폭법을 이용한 서양뒤영벌 의심병원체 Lysinibacillus fusiformis의 신속 검출법

  • Kim, Somin (Department of Life Science, College of Science and Engineering, Kyonggi University) ;
  • Lim, Sujin (Department of Life Science, College of Science and Engineering, Kyonggi University) ;
  • Kim, Jungmin (Department of Life Science, College of Science and Engineering, Kyonggi University) ;
  • Kim, Byounghee (Department of Life Science, College of Science and Engineering, Kyonggi University) ;
  • Tai, Truong A (Department of Life Science, College of Science and Engineering, Kyonggi University) ;
  • Yoon, Byoungsu (Department of Life Science, College of Science and Engineering, Kyonggi University)
  • 김소민 (경기대학교 이공대학 생명과학과) ;
  • 임수진 (경기대학교 이공대학 생명과학과) ;
  • 김정민 (경기대학교 이공대학 생명과학과) ;
  • 김병희 (경기대학교 이공대학 생명과학과) ;
  • ;
  • 윤병수 (경기대학교 이공대학 생명과학과)
  • Received : 2017.09.10
  • Accepted : 2017.09.26
  • Published : 2017.09.30

Abstract

Lysinibacillus fusiformis has been suspected to be a pathogen of Bombus terrestris in Korea since 2008. In this study, we developed the rapid detection method for the L. fusiformis by utilizing the Ultra-rapid PCR. After optimizing of L. fusiformis-specific Ultra-rapid PCR, it can detect the existence of $1.0{\times}10^8$ L. fusiformis-specific DNA molecules in 4 minute and 22 seconds. Even, only 10 molecules could be detected quantitatively using this method. In addition, for the first time, in our knowledge, L. fusiformis was detected using proposed method from bumblebee produced commercially in Korea. Not only in the laboratory but also in the field, L. fusiformis-specific Ultra-rapid PCR would be applied and might be expected as convenient tools at production of bumblebee or inspection for the import and export of bumblebee.

Acknowledgement

Supported by : 농림수산식품기술기획평가원