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Development of Ultra-Rapid Reverse-Transcription PCR for the Rapid Detection against Slow Bee Paralysis Virus (SBPV)

Slow Bee Paralysis Virus (SBPV) 신속 검출을 위한 초고속 역전사 중합효소 연쇄반응법의 개발

  • Kim, Somin (Department of Life Science, Kyonggi University) ;
  • Lim, Sujin (Department of Life Science, Kyonggi University) ;
  • Kim, Jungmin (Department of Life Science, Kyonggi University) ;
  • Lim, Yoon-Kyu (College of Veterinary Medicine, Jeju National University) ;
  • Yoon, Byoungsu (Department of Life Science, Kyonggi University)
  • 김소민 (경기대학교 이공대학 생명과학과) ;
  • 임수진 (경기대학교 이공대학 생명과학과) ;
  • 김정민 (경기대학교 이공대학 생명과학과) ;
  • 임윤규 (제주대학교 수의과대학 산업곤충연구소) ;
  • 윤병수 (경기대학교 이공대학 생명과학과)
  • Received : 2017.09.15
  • Accepted : 2017.09.25
  • Published : 2017.09.30

Abstract

Slow Bee Paralysis Virus (SBPV) is a pathogenic virus against honeybee and bumblebee, causes the death of adult bee by paralyzing the fore-leg of bee. In this study, for rapid detection of SBPV from bumblebee, SBPV-specific Ultra-rapid Reverse transcription PCR was developed. After optimizing of SBPV-specific Ultra-rapid PCR, the existence of $1.0{\times}10^8$ SBPV-specific DNA molecules could be recognized in 3 minute and 35 seconds. Even $1.0{\times}10^1$ molecules of SBPV-specific DNA could be measured with quantitative manner. Meanwhile, from both imported bumblebee and bumblebee produced in Korea, SBPV were detected using proposed method. In the laboratory as well as in the field, SBPV-specific Ultra-rapid Reverse transcription PCR would be applied and might be expected as useful tools at production of bumblebee or inspection for the import and export system of bumblebee.

Acknowledgement

Supported by : 농림수산식품기술기획평가원