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Development of Rapid Detection System for Small Hive Beetle (Aethina tumida) by using Ultra-Rapid PCR

초고속 유전자 증폭법을 이용한 벌집꼬마밑빠진벌레 (Aethina tumida)의 신속한 검출 기법 개발

  • Kim, Jung-Min (Department of Life Science, Kyonggi University) ;
  • Lim, Su-Jin (Department of Life Science, Kyonggi University) ;
  • Tai, Truong A (Department of Life Science, Kyonggi University) ;
  • Hong, Ki-Jeong (Department of Plant Medicine, College of Life Science and Natural Resources, Sunchon National University) ;
  • Yoon, Byoung-Su (Department of Life Science, Kyonggi University)
  • 김정민 (경기대학교 자연과학대학 생명과학과) ;
  • 임수진 (경기대학교 자연과학대학 생명과학과) ;
  • ;
  • 홍기정 (순천대학교 생명산업과학대학 식물의학과) ;
  • 윤병수 (경기대학교 자연과학대학 생명과학과)
  • Received : 2017.06.20
  • Accepted : 2017.06.28
  • Published : 2017.06.30

Abstract

For the Rapid detection of small hive beetle (SHB; Aethina tumida) and for the mass-survey against SHB invasion, SHB-specific ultra-rapid PCR system was developed. Three different pairs of Aethina tumida-specific primers were deduced from cytochrome oxidase subunit I (COI) gene in mitochondrial DNA of SHB. Using optimized SHB-specific ultra-rapid PCR, $2.1{\times}10^1$ molecules of COI gene belonged to SHB could be detected specifically and quantitatively within 18 minutes 40 seconds. For the purpose of the application in apiary field, a DNA extraction method from bee debris was separatedly developed. When $10^5$ SHB-specific COI molecules (1/1000 body of SHB larvae) are existed in 1g of bee debris, it could be verified inner 10 minutes as qualitative and quantitative manner. SHB-specific ultra-rapid PCR we proposed would be expected to apply widely, either in apiary field or laboratory, for the rapid detections and the control against SHB-invasion.

Acknowledgement

Supported by : 농림수산식품기술기획평가원