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Small RNA Transcriptome of Hibiscus Syriacus Provides Insights into the Potential Influence of microRNAs in Flower Development and Terpene Synthesis

  • Kim, Taewook (Department of Agricultural Biotechnology, Seoul National University) ;
  • Park, June Hyun (Department of Agricultural Biotechnology, Seoul National University) ;
  • Lee, Sang-gil (Program in Applied Life Chemistry, Seoul National University) ;
  • Kim, Soyoung (Department of Agricultural Biotechnology, Seoul National University) ;
  • Kim, Jihyun (Program in Applied Life Chemistry, Seoul National University) ;
  • Lee, Jungho (Green Plant Institute) ;
  • Shin, Chanseok (Department of Agricultural Biotechnology, Seoul National University)
  • Received : 2017.05.26
  • Accepted : 2017.07.11
  • Published : 2017.08.31

Abstract

MicroRNAs (miRNAs) are essential small RNA molecules that regulate the expression of target mRNAs in plants and animals. Here, we aimed to identify miRNAs and their putative targets in Hibiscus syriacus, the national flower of South Korea. We employed high-throughput sequencing of small RNAs obtained from four different tissues (i.e., leaf, root, flower, and ovary) and identified 33 conserved and 30 novel miRNA families, many of which showed differential tissuespecific expressions. In addition, we computationally predicted novel targets of miRNAs and validated some of them using 5' rapid amplification of cDNA ends analysis. One of the validated novel targets of miR477 was a terpene synthase, the primary gene involved in the formation of disease-resistant terpene metabolites such as sterols and phytoalexins. In addition, a predicted target of conserved miRNAs, miR396, is SHORT VEGETATIVE PHASE, which is involved in flower initiation and is duplicated in H. syriacus. Collectively, this study provides the first reliable draft of the H. syriacus miRNA transcriptome that should constitute a basis for understanding the biological roles of miRNAs in H. syriacus.

Keywords

development;flowering initiation;Hibiscus syriacus;microRNA;small RNA;terpene synthesis

Acknowledgement

Supported by : Rural Development Administration

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