Effects of Light-emitting Diodes on In Vitro Growth of Virus-free Sweet Potato Plantlets

LED가 고구마 바이러스 무병묘의 기내 생장에 미치는 영향

  • Yoo, Kyoung-Ran (Institute of Life Science and Natural Resources, Wonkwang University) ;
  • Lee, Seung-Yeob (Institute of Life Science and Natural Resources, Wonkwang University)
  • 유경란 (원광대학교 생명자원과학연구소) ;
  • 이승엽 (원광대학교 생명자원과학연구소)
  • Received : 2016.07.22
  • Accepted : 2016.12.19
  • Published : 2017.08.28


The in vitro growth of virus-free sweet potato [Ipomoea batatas (L.) Lam.] plantlets was investigated under different light sources: fluorescent lamp (control); red (660 nm), blue (460 nm), white light-emitting diodes (LED), and two mixtures of blue and red LED (R:B = 8:2, and 7:3). Single node explants (10 mm) of three cultivars ('Matnami', 'Shincheonmi', and 'Yeonhwangmi') were cultured on Murashige and Skoog medium supplemented with $0.2mg{\cdot}L^{-1}$ 6-benzyladenine for 4 weeks. Explants were exposed to $150{\pm}5{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ photosynthetic photon flux at a distance of 20 cm, constant temperature of $25^{\circ}C$, and under 16/8-h (day/night) photoperiod. Using the same method, the in vitro growth of 10 cultivars under red LED was also compared. After 3 weeks, vine length was highest in plantlets cultured under red LED, and lowest in plantlets cultured under blue LED. Fresh and dry weights were also greatest in plantlets cultured under red LED. Compared to the control, vine thickness was significantly higher in plantlets grown under white LED and the 7:3 R:B LED mixture. Significant differences were observed among the 10 cultivars grown under red LED. 'Matnami', 'Shincheonmi', and 'Shinhwangmi' all had excellent vine lengths, and fresh and dry weights. Compared to the control, vine elongation of sweet potato plantlets was most effective under red LED, and culture duration was about 1 week shorter.


Supported by : 원광대학교


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