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Culture Conditions Affecting Spore Germination, Prothallus Propagation and Sporophyte Formation of Dryopteris nipponensis Koidz.

참지네고사리의 포자발아, 전엽체 및 포자체 번식에 영향을 미치는 배양 조건

  • Jang, Bo Kook (Brain Korea 21 Center for Bio-Resource Development, Division of Animal, Horticultural, and Food Sciences, Chungbuk National University) ;
  • Cho, Ju Sung (Major in Horticulture, Division of Animal, Horticultural, and Food Sciences, Chungbuk National University) ;
  • Lee, Ki Cheol (Useful Plant Resources, Korea National Arboretum of the Korea Forest Service) ;
  • Lee, Cheol Hee (Brain Korea 21 Center for Bio-Resource Development, Division of Animal, Horticultural, and Food Sciences, Chungbuk National University)
  • 장보국 (충북대학교 축산.원예.식품공학부 생물건강소재산업화사업단) ;
  • 조주성 (충북대학교 축산.원예.식품공학부 원예학전공) ;
  • 이기철 (국립수목원 유용식물증식센터) ;
  • 이철희 (충북대학교 축산.원예.식품공학부 생물건강소재산업화사업단)
  • Received : 2016.07.18
  • Accepted : 2016.11.17
  • Published : 2017.08.28

Abstract

We investigated a suitable method for in vitro germination of spores, propagation of prothalli, and the formation of sporophytes in the fern Dryopteris nipponensis Koidz. Spore germination rate was relatively high regardless of culture medium. Prothallus development was faster in Knop medium than in Murashige and Skoog (MS) media. Prothalli used in all experiments were obtained from germinated spores, and were cultivated in different concentrations of media components. The active formation of sexual organs such as antheridium made 1MS medium suitable for prothallus propagation, although there was a lower propagation ratio compared to Knop medium. Growth and morphogenesis of prothalli were most effective on 1MS medium containing 2% sucrose, and 60 mM of total nitrogen source with 20:40 mM ratio of $NH_4{^+}:NO_3{^-}$. To select a suitable soil composition for sporophyte formation, ground prothalli were cultivated on single and mixed soils using bed soil, peat moss, perlite, and decomposed granite for 14 weeks. Bed soil promoted sporophyte formation and growth regardless of single or mixed use. In particular, a mixture of bed soil and decomposed granite in a 2:1 ratio (v:v) led to accelerated sporophyte formation ($0.83/cm^2$).

Acknowledgement

Grant : 유용탐사식물자원의 대량증식 및 재배기술 개발

Supported by : 유용식물증식센터

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