DOI QR코드

DOI QR Code

Immuno-Enhancing Effect of Enzymatic Extract of Sargassum coreanum Using Crude Enzyme from Shewanella oneidensis PKA 1008

Shewanella oneidensis PKA 1008 유래 조효소 처리에 의한 큰잎모자반(Sargassum coreanum) 추출 분해물의 면역증진 효과

  • Park, Sun-Hee (Department of Food Science & Technology/Institute of Food Science, Pukyong National University) ;
  • Kim, Min-Ji (Department of Food Science & Technology/Institute of Food Science, Pukyong National University) ;
  • Kim, Go-Eun (Department of Food Science & Technology/Institute of Food Science, Pukyong National University) ;
  • Park, So-Yeong (Department of Food Science & Technology/Institute of Food Science, Pukyong National University) ;
  • Kim, Koth-Bong-Woo-Ri (Department of Food Science & Technology/Institute of Food Science, Pukyong National University) ;
  • Kim, Yeon-Ji (Institute of Fisheries Sciences, Pukyong National University) ;
  • Cho, Young-Je (School of Food Science of Biotechnology, Kyungpook National University) ;
  • Ahn, Dong-Hyun (Department of Food Science & Technology/Institute of Food Science, Pukyong National University)
  • 박선희 (부경대학교 식품공학과/식품연구소) ;
  • 김민지 (부경대학교 식품공학과/식품연구소) ;
  • 김고은 (부경대학교 식품공학과/식품연구소) ;
  • 박소영 (부경대학교 식품공학과/식품연구소) ;
  • 김꽃봉우리 (부경대학교 식품공학과/식품연구소) ;
  • 김연지 (부경대학교 수산과학연구소) ;
  • 조영제 (경북대학교 식품공학부) ;
  • 안동현 (부경대학교 식품공학과/식품연구소)
  • Received : 2017.05.22
  • Accepted : 2017.07.18
  • Published : 2017.08.31

Abstract

The immuno-enhancing effects of alginate oligosaccharides from Sargassum coreanum were investigated. The alginate oligosaccharides were produced by an alginate-degrading enzyme from S. oneidensis PKA 1008. The degraded alginate oligosaccharides were visualized by thin-layer chromatography developed using a solvent system of 1-butanol/methanol/water, 4:1:2 (v/v/v). Alginate was degraded into dimmers at 60 h. As a result, the levels of Th1 cytokine [interferon $(IFN)-{\gamma}$ and interleukin (IL)-2] and Th2 cytokine (IL-6 and IL-10) increased with increasing incubation time compared to the control in vitro. Enzymatic extract treatment promoted proliferation of splenocytes at concentrations of 100 and 200 mg/kg at 24 h in vivo. Secretion of $IFN-{\gamma}$ and IL-2 significantly increased in a dose-dependent manner at 24 h as well as induced higher production of IgG2a in serum. Natural killer cell activity was measured and tended to increase. In addition, complete blood cell counts increased in a dose-dependent manner. These results indicate that alginate oligosaccharides produced by crude enzyme from S. oneidensis PKA 1008 may have significant immune activities.

Acknowledgement

Supported by : 부경대학교

References

  1. Bae JM, Cho EK, Kim HY, Kang SH, Choi YJ. 2012. Biological analysis of enzymatic extracts from Capsosiphon fulvescens using the Microbulbifer sp. AJ-3 marine bacterium. J Life Sci 22: 627-633. https://doi.org/10.5352/JLS.2012.22.5.627
  2. Mizuno H, Saito T, Iso N, Onda N, Noda K, Takada K. 1983. Mannuronic to guluronic acid ratios of alginic acids prepared from various brown seaweeds. Bull Jpn Soc Sci Fish 49: 1591-1593. https://doi.org/10.2331/suisan.49.1591
  3. Joo DS, Lee JS, Park JJ, Cho SY, Ahn CB, Lee EH. 1995. Purification and characterization of the intracellular alginase from Vibrio sp. AL-145. Kor J Appl Microbiol Biotechnol 23: 432-438.
  4. Kim HY, Hong KH, Choi JD, Park SK, Jung SS, Choi WJ, Ahn YS, Hong YP, Song OJ, Moon DC, Lee SH, Shin IS. 2006. Development of analytical method for sodium alginate in foods. Korean J Food Sci Technol 38: 1-4.
  5. Lee JH, Lee EY. 2003. Isolation of alginate-degrading marine bacteria and characterization of alginate. Korean J Life Sci 13: 718-722. https://doi.org/10.5352/JLS.2003.13.5.718
  6. Rehm BH, Valla S. 1997. Bacterial alginates: biosynthesis and applications. Appl Microbiol Biotechnol 48: 281-288. https://doi.org/10.1007/s002530051051
  7. Uo MH, Joo DS, Cho SY, Min TS. 2006. Purification and characterization of the extracellular alginase produced by Bacillus licheniformis AL-577. J Korean Soc Food Sci Nutr 35: 231-237. https://doi.org/10.3746/jkfn.2006.35.2.231
  8. Bark SW, Kim KBWR, Kim MJ, Kang BK, Pak WM, Ahn NK, Choi YU, Park JH, Bae NY, Lim SM, Ahn DH. 2015. Antioxidant effect of enzymatic hydrolysate from Sargassum thunbergii using Vibrio crassostreae PKA 1002 crude enzyme. Microbiol Biotechnol Lett 43: 105-111. https://doi.org/10.4014/mbl.1501.01003
  9. Iwamoto Y, Xu X, Tamura T, Oda T, Muramatsu T. 2003. Enzymatically depolymerized alginate oligomers that cause cytotoxic cytokine production in human mononuclear cells. Biosci Biotechnol Biochem 67: 258-263. https://doi.org/10.1271/bbb.67.258
  10. Sunwoo C, Kim KBWR, Kim DH, Jung SA, Kim HJ, Jeong DH, Jung HY, Kang BK, Bark SW, Lim SM, Hong YK, Ahn DH. 2013. Optimization of conditions for the production and properties of alginate-degrading crude enzyme from Shewanella oneidensis PKA 1008. Korean J Microbiol Biotechnol 41: 372-378. https://doi.org/10.4014/kjmb.1212.12002
  11. Kim MJ, Bae NY, Bark SW, Kim KBWR, Park JH, Park SH, Ahn DH. 2015. Anti-inflammatory effect of alginate oligosaccharides produced by an alginate-degrading enzyme from Shewanella oneidensis PKA1008 on LPS-induced RAW 264.7 cells. Korean J Fish Aquat Sci 48: 888-897.
  12. Yang HP. 2007. Antioxidant and antitumor activities of enzymatic extracts from Sargassum coreanum. PhD Dissertation. Jeju National University, Jeju, Korea.
  13. Kang BK, Kim KBWR, Kim MJ, Bark SW, Pak WM, Ahn NK, Choi YU, Bae NY, Park JH, Ahn DH. 2015. Anti-inflammatory effect of Sargassum coreanum ethanolic extract through suppression of NF-${\kappa}B$ pathway in LPS-induced RAW264.7 cells and mouse. Microbiol Biotechnol Lett 43: 112-119. https://doi.org/10.4014/mbl.1504.04008
  14. Athukorala Y, Lee KW, Kim SK, Jeon YJ. 2007. Anticoagulant activity of marine green and brown algae collected from Jeju Island in Korea. Bioresour Technol 98: 1711-1716. https://doi.org/10.1016/j.biortech.2006.07.034
  15. Ko SC, Kang SM, Ahn G, Yang HP, Kim KN, Jeon YJ. 2010. Antioxidant activity of enzymatic extracts from Sargassum coreanum. J Korean Soc Food Sci Nutr 39: 494-499. https://doi.org/10.3746/jkfn.2010.39.4.494
  16. Park YM, Won JH, Yun KJ, Ryu JH, Han YN, Choi SK, Lee KT. 2006. Preventive effect of Ginkgo biloba extract (GBB) on the lipopolysaccharide-induced expressions of inducible nitric oxide synthase and cyclooxygenase-2 via suppression of nuclear factor-${\kappa}B$ in RAW 264.7 cells. Biol Pharm Bull 29: 985-990. https://doi.org/10.1248/bpb.29.985
  17. Wong TY, Preston LA, Schiller NL. 2000. Alginate lyase: review of major sources and enzyme characteristics, structure-function analysis, biological roles, and applications. Annu Rev Microbiol 54: 289-340. https://doi.org/10.1146/annurev.micro.54.1.289
  18. Zhu Y, Wu L, Chen Y, Ni H, Xiao A, Cai H. 2016. Characterization of an extracellular biofunctional alginate lyase from marine Microbulbifer sp. ALW1 and antioxidant activity of enzymatic hydrolysates. Microbiol Res 182: 49-58. https://doi.org/10.1016/j.micres.2015.09.004
  19. Scharton TM, Scott P. 1993. Natural killer cells are a source of interferon gamma that drives differentiation of $CD4^+$ T cell subsets and induces early resistance to Leishmania major in mice. J Exp Med 178: 567-577. https://doi.org/10.1084/jem.178.2.567
  20. Vivier E, Tomasello E, Baratin M, Walzer T, Ugolini S. 2008. Functions of natural killer cells. Nat Immunol 9: 503-510. https://doi.org/10.1038/ni1582
  21. Abbs AK, Lichtman AH. 2003. Cells and tissues of the immune system. In Cellular and Molecular Immunology. 5th ed. Jason M, Bill S, eds. WB Saunders Company, Philadelphia, PA, USA. p 32-33,264-269.
  22. Mosmann TR, Cherwinski H, Bond MW, Giedlin MA, Coffman RL. 1986. Two types of murine helper T cell clone. I. Definition according to profiles of lymphokine activities and secreted proteins. J Immunol 136: 2348-2357.
  23. Kishimoto T, Akira S, Narazaki M, Taga T. 1995. Interleukin-6 family of cytokines and gp130. Blood 86: 1243-1254.
  24. Dienz O, Rincon M. 2009. The effects of IL-6 on CD4 T cell responses. Clin Immunol 130: 27-33. https://doi.org/10.1016/j.clim.2008.08.018
  25. Fiorentino DF, Zlotnik A, Vieira P, Mosmann TR, Howard M, Moore KW, O’Garra A. 1991. IL-10 acts on the antigen-presenting cell to inhibit cytokine production by Th1 cells. J Immunol 146: 3444-3451.
  26. Mao XF, Piao XS, Lai CH, Li DF, Xing JJ, Shi BL. 2005. Effect of ${\beta}$-glucan obtained from the Chinese herb Astragalus membranaceus and lipopolysaccharide challenge on performance, immunological, adrenal, and somatotropic responses of weanling pigs. J Anim Sic 83: 2775-2782. https://doi.org/10.2527/2005.83122775x
  27. Clerici M, Ferrario E, Trabattoni D, Viviani S, Bonfanti V, Venzon DJ, Clerici E, Shearer GM, Villa ML. 1994. Multiple defects of T helper cell function in newly diagnosed patients with Hodgkin's disease. Eur J Cancer 30A: 1464-1470.
  28. Male D, Brostoff J, Roth DB, Roitt I. 2006. Cells, tissues, and organs of the immune system. In Immunology. 7th ed. Mosby, St. Louis, MO, USA. p 19-58.
  29. Lee HJ, Lim SY, Kang MG, Park J, Chung HJ, Yang SJ. 2015. Beneficial effects of Daebong persimmon against oxidative stress, inflammation, and immunity in vivo. J Korean Soc Food Sci Nutr 44: 491-496. https://doi.org/10.3746/jkfn.2015.44.4.491
  30. Biron CA, Nguyen KB, Pien GC, Cousens LP, Salazar-Mather TP. 1999. Natural killer cells in antiviral defense: function and regulation by innate cytokines. Annu Rev Immunol 17: 189-220. https://doi.org/10.1146/annurev.immunol.17.1.189
  31. Frese-Schaper M, Keil A, Yagita H, Steiner SK, Falk W, Schmid RA, Frese S. 2014. Influence of natural killer cells and perforin-mediated cytolysis on the development of chemically induced lung cancer in A/J mice. Cancer Immunol Immunother 63: 571-580. https://doi.org/10.1007/s00262-014-1535-x
  32. Ha Y, Kim OK, Nam DE, Kim Y, Kim E, Jun W, Lee J. 2015. Effect of Curcuma longa L. extracts on natural killer cells and T cells. J Korean Soc Food Sci Nutr 44: 307-313. https://doi.org/10.3746/jkfn.2015.44.3.307