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Evaluations on Antioxidant Effect of Water Extract from Graviola Leaves

그라비올라잎 열수추출물의 항산화 효능 평가

  • Choi, Jong-Hwa (Interdisciplinary Program of Perfume and Cosmetics, Graduate School of Chonnam National University) ;
  • Ohk, Seung-Ho (Interdisciplinary Program of Perfume and Cosmetics, Graduate School of Chonnam National University)
  • 최종화 (전남대학교 대학원 향장품학 협동과정) ;
  • 옥승호 (전남대학교 대학원 향장품학 협동과정)
  • Received : 2017.04.12
  • Accepted : 2017.06.09
  • Published : 2017.06.30

Abstract

This study examined the antioxidant activity of the water extract from graviola leaves to develop a harmless and highly stable natural antioxidant. The total polyphenol content, total flavonoid content, DPPH radical scavenging activity, and MTT assay activity were measured. As a result, 62.3 g of the water extract from graviola leaves was obtained at $98^{\circ}C$ using 300 g of graviola leaf powder. The total polyphenol content was $291.97+2.39{\mu}g/mL$ and the total flavnoid content was $161{\pm}7.85{\mu}g/mL$ in a 1 mg/mL water extract from graviola leaves. The DPPH radical scavenging activity showed 51.6%, 67.8%, 79%, 82.4% and 83.9% at concentrations of 1, 2.5, 5, 10 and 15 mg/mL. This shows concentration-dependent scavenging activity and significant antioxidant activity. As a result of measuring the toxicity about HDF cells, a HDF cell survival rate of 100% was observed at a 150 mg /mL concentration, which was the same as that of the control group and a higher cell survival rate at a lower concentration. In conclusion, the graviola leaf extract can be developed as a material of food or cosmetics containing natural antioxidants.

Keywords

antioxidant activity;flavonoid;Graviola leaves;Human Dermal Fibroblasts (HDF cell);polyphenol

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