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Production of siderophore from L-glutamic acid as both carbon and nitrogen sole sources in Acinetobacter sp. B-W

글루탐산을 유일한 탄소원과 질소원으로 이용하는 Acinetobacter sp. B-W의 시드로포어 생산

  • Kim, Kyoung-Ja (Department of life Science and Biotechnology, College of Natural Science, Soonchunhyang University) ;
  • Jang, Ju-Ho (Department of life Science and Biotechnology, College of Natural Science, Soonchunhyang University) ;
  • Yang, Yong-Joon (Department of Plant and Food Science, Sangmyung University)
  • 김경자 (순천향대학교 생명시스템학과) ;
  • 장주호 (순천향대학교 생명시스템학과) ;
  • 양용준 (상명대학교 식물식품공학과)
  • Received : 2017.04.12
  • Accepted : 2017.05.22
  • Published : 2017.06.30

Abstract

Catechol type siderophore different from 2, 3-dihydroxybenzoic acid (DHB) was produced from Acinetobacter sp. B-W grown in medium containing L-glutamic acid as both carbon and nitrogen sole sources at $28^{\circ}C$. Optimal concentration of glutamic acid for siderophore production was 3% and production of siderophore was decreased above 3% glutamic acid. In previous report, siderophore, 2, 3-DHB was produced from strain B-W grown in medium containing glucose as carbon source and glutamic acid as nitrogen source. Rf value of siderophore produced from strain B-W grown in medium glutamic acid as both carbon and nitrogen sole sources at $28^{\circ}C$ was 0.32, while 2, 3-DHB was 0.84 in butanol-acetic acid-water (12:3:5) as developing solvent. Antioxidative activity of 2, 3-DHB was not detected in that siderophore produced from glutamic acid. Catechol nature of siderophore was detected by Arnow test. Although in iron-limited media optimal cell growth was identified at $36^{\circ}C$, significant quantities of siderophore were produced only at $28^{\circ}C$. Biosynthesis of siderophore was strongly inhibited by growth at $36^{\circ}C$. Production of siderophore was completely inhibited by $10{\mu}M\;FeCl_3$.

Keywords

Acinetobacter sp. B-W;L-glutamic acid;siderophore;sole carbon and nitrogen sources

Acknowledgement

Supported by : Soonchunhyang University

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