Eco-friendly and efficient in situ restoration of the constructed sea stream by bioaugmentation of a microbial consortium

복합미생물 생물증강법을 이용한 인공해수하천의 친환경 효율적 현장 수질정화

  • Yoo, Jangyeon (Department of Convergence Study on Ocean Science and Technology, Korea Institute of Ocean Science and Technology) ;
  • Kim, In-Soo (Department Environmental Engineering, Korea Maritime and Ocean University) ;
  • Kim, Soo-Hyeon (Department Civil and Environmental Engineering, Graduate School of Korea Maritime and Ocean University) ;
  • Ekpeghere, Kalu I. (Department Civil and Environmental Engineering, Graduate School of Korea Maritime and Ocean University) ;
  • Chang, Jae-Soo (Department Environmental Engineering, Korea Maritime and Ocean University) ;
  • Park, Young-In (Division of Public Health and Environment, Kosin University) ;
  • Koh, Sung-Cheol (Department Environmental Engineering, Korea Maritime and Ocean University)
  • 유장연 (한국해양과학기술전문대학원 해양과학기술융합학과) ;
  • 김인수 (한국해양대학교 환경공학과) ;
  • 김수현 (한국해양대학교 대학원 토목환경공학과) ;
  • 칼루 엑페게어 (한국해양대학교 대학원 토목환경공학과) ;
  • 장재수 (한국해양대학교 환경공학과) ;
  • 박영인 (고신대학교 보건환경학부) ;
  • 고성철 (한국해양대학교 환경공학과)
  • Received : 2017.06.09
  • Accepted : 2017.06.21
  • Published : 2017.06.30


A constructed sea stream in Yeongdo, Busan, Republic of Korea is mostly static due to the lifted stream bed and tidal characters, and receives domestic wastewater nearby, causing a consistent odor production and water quality degradation. Bioaugmentation of a microbial consortium was proposed as an effective and economical restoration technology to restore the polluted stream. The microbial consortium activated on site was augmented on a periodic basis (7~10 days) into the most polluted site (Site 2) which was chosen considering the pollution level and tidal movement. Physicochemical parameters of water qualities were monitored including pH, temperature, DO, ORP, SS, COD, T-N, and T-P. COD and microbial community analyses of the sediments were also performed. A significant reduction in SS, COD, T-N, and COD (sediment) at Site 2 occurred showing their removal rates 51%, 58% and 27% and 35%, respectively, in 13 months while T-P increased by 47%. In most of the test sites, population densities of sulfate reducing bacterial (SRB) groups (Desulfobacteraceae_uc_s, Desulfobacterales_uc_s, Desulfuromonadaceae_uc_s, Desulfuromonas_g1_uc, and Desulfobacter postgatei) and Anaerolinaeles was observed to generally decrease after the bioaugmentation while those of Gamma-proteobacteria (NOR5-6B_s and NOR5-6A_s), Bacteroidales_uc_s, and Flavobacteriales_uc_s appeared to generally increase. Aerobic microbial communities (Flavobacteriaceae_uc_s) were dominant in St. 4 that showed the highest level of DO and least level of COD. These microbial communities could be used as an indicator organism to monitor the restoration process. The alpha diversity indices (OTUs, Chao1, and Shannon) of microbial communities generally decreased after the augmentation. Fast uniFrac analysis of all the samples of different sites and dates showed that there was a similarity in the microbial community structures regardless of samples as the augmentation advanced in comparison with before- and early bioaugmentation event, indicating occurrence of changing of the indigenous microbial community structures. It was concluded that the bioaugmentation could improve the polluted water quality and simultaneously change the microbial community structures via their niche changes. This in situ remediation technology will contribute to an eco-friendly and economically cleaning up of polluted streams of brine water and freshwater.


Flavobacteriaceae;bioaugmentation;microbial consortium;polluted stream;pyrosequencing;restoration;sulfate reducing bacteria (SRB)


Supported by : Busan Green Environment Center


  1. Ahn, T.W., Choi, I.S., and Oh, J.M. 2009. A study on the quality improvement of secondary treatment effluent utilize the natural purification method. Environ. Impact Assess. 18, 79-87.
  2. Ahn, S.E. and Kim, G. 2016. Economic values of freshwater ecosystem services from demand and supply perspectives. J. Korean Soc. Environ. Eng. 38, 580-587.
  3. American Public Health Association (APHA). 2005. American Water Works Association, Water Environment Federation. Standard Methods for the Examination of Water and Wastewater, 21st Ed.; Authors: Washington, DC, USA.
  4. Biovankorea. 2010. Microbial agent effective in wastewater treatment and its manufacturing methods and wastewater treatment technology. Korea Patent. No. 10-2010-0089906.
  5. Blazejak, A. and Schippers, A. 2011. Real-time PCR quantification and diversity analysis of the functional genes aprA and dsrA of sulfate-reducing prokaryotes in marine sediments of the Peru continental margin and the Black Sea. Front. Microbiol. 2, 253-279.
  6. Cankovic, M., Petric, I., Margus, M., and Ciglenecki, I. 2017. Spatiotemporal dynamics of sulfate-reducing bacteria in extreme environment of Rogoznica Lake revealed by 16S rRNA analysis J. Mar. Syst. 172, 14-23.
  7. Chang, J.S., Song, J., Kim, I.S., Yoo, J.Y., and Koh, S.C. 2015. Eco-friendly remediation and odor control of a contaminated urban stream using beneficial microorganisms. Korean J. Microbiol. 51, 389-397.
  8. Chao, A. 1984. Non-parametric estimation of the number of classes in a population. Scand. J. Stat. 11, 265-270.
  9. Chon, T.S., Qu, X., Cho, W.S., Hwang, H.J., Tang, H., Liu, Y., Choi, J.H., Jung, M., Chung, B.S., Lee, H.Y., et al. 2013. Evaluation of stream ecosystem health and species association based on multitaxa (benthic macroinvertebrates, algae, and microorganisms) patterning with different levels of pollution. Ecol. Inform. 17, 58-72.
  10. Chung, Y.J. and Im, K.S. 2006. Purification of stream water quality by using rope media filter. J. Korean Soc. Water Qual. 22, 238-243.
  11. Ekpeghere, K.I., Bae, H.J., Kwon, S.H., Kim, B.H., Park, D.J., and Koh, S.C. 2009. Clean-up of the crude oil contaminated marine sediments through biocarrier-mediated bioaugmentation. Korean J. Microbiol. 45, 354-361.
  12. Ekpeghere, K.I., Kim, B.H., Son, H.S., Whang, K.S., Kim, H.S., and Koh, S.C. 2012. Functions of effective microorganisms in bioremediation of the contaminated harbor sediments. J. Environ. Sci. Health A Tox. Hazard Subst. Environ. Eng. 47, 44-53.
  13. Hamady, M., Lozupone, C., and Knight, R. 2010. Fast Uni Frac: Facilitating high-throughput phylogenetic analyses of microbial communities including analysis of pyrosequencing and Phylo Chip data. ISME J. 4, 17-27.
  14. Herrero, M. and Stuckey, D.C. 2015 Bioaugmentation and its application in wastewater treatment: A review. Chemosphere 140, 119-128.
  15. Jiao, Y., Zhao, Q., Jin, W., Hao, X., and You, S. 2011. Bioaugmentation of a biological contact oxidation ditch with indigenous nitrifying bacteria for in situ remediation of nitrogen-rich stream water. Bioresour. Technol. 102, 990-995.
  16. Jung, S.R. and Lin, Y.F. 2004. Seasonal effect on ammonia nitrogen removal by constructed wetlands treating polluted river water in Southern Taiwan. Environ. Pollut. 127, 291-301.
  17. Kim, S.J., Choi, Y.S., and Bae, W.G. 2006. Application of hybrid constructed wetland system for stream water quality improvement. Korean Water Environ. Assoc. 22, 209-214.
  18. Kim, M.K., Choi, J.S., Kim, S.J., and Kim, H.G. 2013. Improvement of medium and small urban stream water quality and applicability of design factor using biological and physicochemical processing. J. Kor. Soc. Environ. Eng. 35, 509-517.
  19. Kim, I.S., Ekpeghere, K.I., Ha, S.Y., Kim, B.S., Song, B., Chun, J., Kim, J.T., Kim, H.G., and Koh, S.C. 2014. Full-scale biological treatment of tannery wastewater using the novel microbial consortium BM-S-1. J. Environ. Sci. Health A Tox. Hazard Subst. Environ. Eng. 49, 355-364.
  20. Kim, S.J. and Lee, S.S. 2010. The development of treatment system for removing the low concentrated nitrogen and phosphorus using phototrophic bacteria and media. Korean J. Microbiol. 46, 27-32.
  21. Kim, K. and Yeo, H.K. 2004. A river restoration program based on a close-to-nature river improvement process in Korea. Adv. Hydro-Sci. Engineer. 4.
  22. Lenk, S., Arnds, J., Zerjatke, K., Musat, N., Amann, R., and Musmann, M. 2011. Novel groups of Gammaproteobacteria catalyse sulfur oxidation and carbon fixation in a coastal, intertidal sediment. Environ. Microbiol. 13, 758-774.
  23. Liu, B., Giannis, A., Zhang J., Chang, V.W.C., and Wang, J.Y. 2013. Characterization of induced struvite formation from sourceseparated urine using seawater and brine as magnesium sources. Chemosphere 93, 2738-2747.
  24. Luisa, W.M., Letícia, T., Francielle, B., Raquel, D., Patricia, D.Q., Kateryna, Z., Jennifer, D., Robson, A., Eric, W.T., Ana, P., et al. 2015. Culture-independent analysis of bacterial diversity during bioremediation of soil contaminated with a diesel-biodiesel blend (B10)S. J. Bioremed. Biodegrad. 6. doi:10.4172/2155-6199.1000318.
  25. Ma, F., Guo, J.B., Zhao, L.J., Chang, C.C., and Cu, D. 2009. Application of bioaugmentation to improve the activated sludge system into the contact oxidation system treating petrochemical wastewater. Bioresour. Technol. 100, 597-602.
  26. McBride, M.J. 2014. The family Flavobacteriaceae. The Prokaryotes. pp. 643-676.
  27. Ministry of Environment. 2017. Regulatory Standards for Stream Water Quality.
  28. Nogales, B., Lanfranconi, M.P., Pina-Villalonga, J.M., and Bosch, R. 2011. Anthropogenic pertur bations in marine microbial communities. FEMS Microbiol. Rev. 35, 275-298.
  29. Oh, Y.M., Yee, J.H., Pak, J.J., Choi, K.J., Pak T.J., and Yee, T.H. 2010. Water quality improvement of stagnant water using an upflow activated carbon biofilm process and microbial community analysis. J. Korean Soc. Environ. Eng. 32, 1191-1200.
  30. Park, J.S., Kim, B.K., Kim, W.S., Seo, D.S., and Kim, W.J. 2014. Investigation on water purification effect through long-term continuous flow test of porous concrete using effective microorganisms. J. Korea Concr. Inst. 26, 219-227.
  31. Saeed, T. and Sun, G.Z. 2011. Enhanced denitrification and organics removal in hybrid wetland columns: comparative experiments. Bioresour. Technol. 102, 967-974.
  32. Shannon, C.E. 1948. A mathematical theory of communication. Bell Syst. Tech. J. 27, 379-423 and 623-656.
  33. Tamaki, H., Hanada, S., Kamagata, Y., Nakamura, K., Nomura, N., Nakano, K., and Matsumura1, M. 2003. Flavobacterium limicola sp. nov., a psychrophilic, organic-polymer-degrading bacterium isolated from freshwater sediments. Int. J. Syst. Evol. Microbiol. 53, 519-526.
  34. Tang, W., Zhang, W., Zhao, Y., Wang, Y., and Shan, B. 2013. Ecological nitrogen removal from polluted river water in a novel ditch-wetland-pond system. Ecol. Eng. 60, 135-139.
  35. Tian, X., Wang, G., Guan, D., Li, J., Wang, A., Li, J., Yu, Z., Chen, Y., and Zhang, Z. 2016. Reverse osmosis brine for phosphorus recovery from source separated urine. Chemosphere 165, 202-210.
  36. Tortosa, G., Correa, D., Sanchez-Raya, A.J., Delgado, A., Sáchez- Monedero, M.A., and Bedmar, E.J. 2011. Effects of nitrate contamination and seasonal variation on the denitrification and greenhouse gas production in La Rocina Stream (Donana National Park, SW Spain). Ecol. Eng. 37, 539-548.
  37. Wallenstein, M.D., Myrold, D.D., Firestone, M., and Voytek, M. 2006. Environmental controls on denitrifying communities and denitrification rates: insights from molecular methods. Ecol. Appl. 16, 2143-2152.[2143:ECODCA]2.0.CO;2
  38. Wang, J., Gong B., Huang, W., Wang, Y., and Zhou, J. 2017. Bacterial community structure in simultaneous nitrification, denitrification and organic matter removal process treating saline mustard tuber wastewater as revealed by 16S rRNA sequencing. Bioresour. Technol. 228, 31-38.
  39. Wang, Y., Sheng, H.F., He, Y., Wu, J.Y., Jiang, Y.X., Tam, N.F.Y., and Zhoua, H.W. 2012. Comparison of the levels of bacterial diversity in freshwater, intertidal wetland, and marine sediments by using millions of illumina tags. J. Appl. Environ. Microbiol. 78, 8264-8271.
  40. Yang, K.H. 2003. Water treatment system using titanium bioball. Korea Patent. No. 10-2003-0102359.
  41. Yao, S., Chen, L., Guan, D., Zhang, Z., Tian, X., Wang, A., Wang, G., Yao, Q., Peng, D., and Li, J. 2017. On-site nutrient recovery and removal from source-separated urine by phosphorus precipitation and short-cut nitrification-denitrification. Chemosphere 175, 210-218.