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Decolorization of dyes by a purified laccase from Coprinus comatus

정제된 먹물버섯(Coprinus comatus) laccase에 의한 염료 탈색

  • Kim, Su Yeon (Department of Biochemistry, Kangwon National University) ;
  • Choi, Ji Young (Department of Biochemistry, Kangwon National University) ;
  • Choi, Hyoung T. (Department of Biochemistry, Kangwon National University)
  • 김수연 (강원대학교 자연과학대학 생화학과) ;
  • 최지영 (강원대학교 자연과학대학 생화학과) ;
  • 최형태 (강원대학교 자연과학대학 생화학과)
  • Received : 2017.04.04
  • Accepted : 2017.05.17
  • Published : 2017.06.30

Abstract

An inky cap, Coprinus comatus synthesizes and secretes a laccase in the liquid yeast extract peptone dextrose medium. We have successfully purified the enzyme through the ion-exchange chromatography and the preparative gel electrophoresis. The estimated molecular weight was 67 kDa by the SDS-PAGE analysis. Optimum pH was pH 4.3 and optimum temperature was $25^{\circ}C$. The Km value was 0.45 mM and the Vmax was 0.0132 OD/min/unit for o-tolidine. Purified laccase showed 49.3% decolorizing activity against remazol brilliant blue R (RBBR) and 41.6% decolorizing activity against Poly R-478 after 12 h incubation.

Keywords

Coprinus comatus;dye decolorization;laccase

Acknowledgement

Supported by : 강원대학교

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