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Effects of Water Stress on Carotenoid and Proline Contents in Kale (Brassica oleracea var. acephala) leaves

수분스트레스가 케일 잎의 카로티노이드 및 프롤린 함량에 미치는 영향

  • Lee, Hyo-Joon (Department of Bio-Environmental Chemistry, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Chun, Jin-Hyuk (Department of Bio-Environmental Chemistry, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Kim, Sun-Ju (Department of Bio-Environmental Chemistry, College of Agriculture and Life Sciences, Chungnam National University)
  • 이효준 (충남대학교 농업생명과학대학 생물환경화학과) ;
  • 천진혁 (충남대학교 농업생명과학대학 생물환경화학과) ;
  • 김선주 (충남대학교 농업생명과학대학 생물환경화학과)
  • Received : 2010.05.30
  • Accepted : 2010.06.22
  • Published : 2017.06.30

Abstract

BACKGROUND : Environmental stress has a major effect on the growth and yields of vegetables, and can significantly affect nutritionally important phytochemicals, causing large economic losses. METHODS AND RESULTS : The present study was aimed at exploring the effects of water stress on the carotenoid and proline contents in kale leaves to understand drought tolerance of kale plants. Kale was randomly divided into two groups at 57 days after sowing (DAS). One of the groups was well-watered (WW) and the other was water stressed (WS). Harvesting of kale leaves was started one day after treatment (58 DAS) and continued for 10 days (~67 DAS). We investigated the status of plant growth (leaf number, length, width, fresh weight) of kale throughout the study. Carotenoid (lutein, ${\alpha}-carotene$, zeaxanthin, ${\beta}-carotene$) and proline contents were analyzed by high-performance liquid chromatography (HPLC). Our results showed that the total carotenoid contents ranged from 926.0 to 1,212.0 mg/kg dry wt. (at 3 and 2 days, respectively) in WW treatment and 887.8 to 1,157.4 mg/kg dry wt. (at 10 and 4 days, respectively) in WS treatment. The ratio of individual carotenoid to the total carotenoid contents of kale leaves was 51.4 for lutein, 4.44 for zeaxanthin, 2.76 for ${\alpha}-carotene$, and 41.4% for ${\beta}-carotene$. Total carotenoid contents showed a significant reduction from 7 days (1,037.2 mg/kg dry wt.) to 10 days (887.8 mg/kg dry wt.) in WS treatment. The lutein content did not show a significant difference in WW between 7 and 10 days after treatment but showed a significant difference in WS treatment. The ${\alpha}-carotene$ content showed no significant difference between the treatments. However, zeaxanthin content was higher during 4-10 days and ${\beta}-carotene$ content was lower during 6-10 days in WS than in WW on each harvest day. In WW, the proline content showed no significant difference, but in WS, the proline content started to increase at 7 days and almost doubled in 10 days. CONCLUSION : The marked increase in zeaxanthin and proline contents in kale leaves indicated that the two phytochemicals are associated with drought tolerance in the plant.

Keywords

Carotenoids;HPLC analysis;Kale;Proline;Water stress

Acknowledgement

Supported by : Rural Development Administration

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