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UPLC-DAD-QTOF/MS를 이용한 국내 재배 블루베리(Vaccinium corymbosum)와 복분자(Rubus coreanus)의 플라보노이드 특성 비교

Comparison of Flavonoid Characteristics between Blueberry (Vaccinium corymbosum) and Black Raspberry (Rubus coreanus) Cultivated in Korea using UPLC-DAD-QTOF/MS

  • 김영진 (국립농업과학원 농식품자원부 기능성식품과) ;
  • 김헌웅 (국립농업과학원 농식품자원부 기능성식품과) ;
  • 이민기 (국립농업과학원 농식품자원부 기능성식품과) ;
  • 이선혜 (국립농업과학원 농식품자원부 기능성식품과) ;
  • 장환희 (국립농업과학원 농식품자원부 기능성식품과) ;
  • 황유진 (국립농업과학원 농식품자원부 기능성식품과) ;
  • 최정숙 (국립농업과학원 농식품자원부 기능성식품과) ;
  • 이성현 (국립농업과학원 농식품자원부 기능성식품과) ;
  • 차연수 (전북대학교 생활과학대학 식품영양학과) ;
  • 김정봉 (국립농업과학원 농식품자원부 기능성식품과)
  • Jin, Young (Department of Agro-food Resources, National Institute of Agricultural Sciences) ;
  • Kim, Heon-Woong (Department of Agro-food Resources, National Institute of Agricultural Sciences) ;
  • Lee, Min-Ki (Department of Agro-food Resources, National Institute of Agricultural Sciences) ;
  • Lee, Seon-Hye (Department of Agro-food Resources, National Institute of Agricultural Sciences) ;
  • Jang, Hwan-Hee (Department of Agro-food Resources, National Institute of Agricultural Sciences) ;
  • Hwang, Yu-Jin (Department of Agro-food Resources, National Institute of Agricultural Sciences) ;
  • Choe, Jeong-Sook (Department of Agro-food Resources, National Institute of Agricultural Sciences) ;
  • Lee, Sung-Hyun (Department of Agro-food Resources, National Institute of Agricultural Sciences) ;
  • Cha, Youn-Soo (Department of Food Science and Human Nutrition, College of Human Ecology, Chonbuk National University) ;
  • Kim, Jung-Bon (Department of Agro-food Resources, National Institute of Agricultural Sciences)
  • 투고 : 2017.05.23
  • 심사 : 2017.06.21
  • 발행 : 2017.06.30

초록

UPLC-DAD-QTOF/MS를 이용하여 국내에서 재배한 7품종의 하이부시 블루베리와 7지역에서 재배된 복분자 열매에서 총 29종 (블루베리 29종, 복분자 종4)의 개별 플라보노이드 성분을 정성 및 정량 분석하였다. 블루베리의 평균 플라보노이드 함량은 143.0 mg/100g DW로 관찰되었고, 'Darrow'에서 가장 높았으며, 'Nelson'이 가장 낮은 것으로 나타났다. 복분자의 평균 플라보노이드 함량은 95.4 mg/100g DW로 나타났으며, 총 플라보노이드 함량은 정읍>순창>고창>광양 순으로 높았으나 정읍, 순창, 고창간에 유의적인 함량차이는 나타나지 않았다. 블루베리는 hyperoside와 isoqercitrin이 각각 총 플라보노이드 함량 중 평균 31.4%, 13.3%을 차지하였으며, 복분자는 rutin과 miquelianin이 각각 총 플라보노이드 함량 중 평균 51.4%, 40.2%를 차지하여 두 베리류 열매간 주요 플라보노이드 성분의 특성 차이를 확인할 수 있었다. 블루베리 열매에서 quercetin 3-O-robinobioside, quercetin 3-O-(6"-O-malonyl)glucoside, isorhamnetin 3-O-robinobioside, avicularin, kaempferol 3-O-(6"-O-acetyl)glucoside, quercetin이 처음으로 동정되었다. 본 연구 결과는 국내산 블루베리와 복분자를 이용한 기능성 식품 개발에 필요한 기초 자료로 활용될 수 있을 것으로 판단되며, 추후 연구에서는 보다 다양한 베리류에 대한 추가 분석이 필요할 것으로 생각된다. 또한 본 연구 방법은 베리류 뿐만 아니라 다양한 식물에 존재하는 플라보노이드의 정성 및 정량 분석이 가능하여 활용 가치가 높을 것으로 사료된다.

키워드

Flavonoid;Rubus coreanus;UPLC-DAD-QTOF/MS;Vaccinium corymbosum

과제정보

연구 과제 주관 기관 : Rural Development Administration

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