Comparison of Flavonoid Characteristics between Blueberry (Vaccinium corymbosum) and Black Raspberry (Rubus coreanus) Cultivated in Korea using UPLC-DAD-QTOF/MS

UPLC-DAD-QTOF/MS를 이용한 국내 재배 블루베리(Vaccinium corymbosum)와 복분자(Rubus coreanus)의 플라보노이드 특성 비교

  • Jin, Young (Department of Agro-food Resources, National Institute of Agricultural Sciences) ;
  • Kim, Heon-Woong (Department of Agro-food Resources, National Institute of Agricultural Sciences) ;
  • Lee, Min-Ki (Department of Agro-food Resources, National Institute of Agricultural Sciences) ;
  • Lee, Seon-Hye (Department of Agro-food Resources, National Institute of Agricultural Sciences) ;
  • Jang, Hwan-Hee (Department of Agro-food Resources, National Institute of Agricultural Sciences) ;
  • Hwang, Yu-Jin (Department of Agro-food Resources, National Institute of Agricultural Sciences) ;
  • Choe, Jeong-Sook (Department of Agro-food Resources, National Institute of Agricultural Sciences) ;
  • Lee, Sung-Hyun (Department of Agro-food Resources, National Institute of Agricultural Sciences) ;
  • Cha, Youn-Soo (Department of Food Science and Human Nutrition, College of Human Ecology, Chonbuk National University) ;
  • Kim, Jung-Bon (Department of Agro-food Resources, National Institute of Agricultural Sciences)
  • 김영진 (국립농업과학원 농식품자원부 기능성식품과) ;
  • 김헌웅 (국립농업과학원 농식품자원부 기능성식품과) ;
  • 이민기 (국립농업과학원 농식품자원부 기능성식품과) ;
  • 이선혜 (국립농업과학원 농식품자원부 기능성식품과) ;
  • 장환희 (국립농업과학원 농식품자원부 기능성식품과) ;
  • 황유진 (국립농업과학원 농식품자원부 기능성식품과) ;
  • 최정숙 (국립농업과학원 농식품자원부 기능성식품과) ;
  • 이성현 (국립농업과학원 농식품자원부 기능성식품과) ;
  • 차연수 (전북대학교 생활과학대학 식품영양학과) ;
  • 김정봉 (국립농업과학원 농식품자원부 기능성식품과)
  • Received : 2017.05.23
  • Accepted : 2017.06.21
  • Published : 2017.06.30


BACKGROUND: The objective of this study was to identify and compare the main phenolic compounds (anthocyanins, flavonoids, phenolic acids) in blueberry and black raspberry cultivated in Korea using ultra-performance liquid chromatography diode array detection-quadrupole time-of-flight mass spectrometry (UPLC-DAD-QTOF/MS). METHODS AND RESULTS: Twenty-nine flavonoids were identified by comparison of ultraviolet and mass spectra with data in a chemical library and published data. Blueberry contained flavonols including kaempferol, quercetin, isorhamnetin, myricetin, and syringetin aglycones. Isorhamnetin 3-O-robinobioside, kaempferol 3-O-(6"-O-acetyl)glucoside, quercetin, quercetin 3-O-arabinofuranoside (avicularin), quercetin 3-O-(6''-O-malonyl) glucoside, and quercetin 3-O-robinobioside were detected for the first time in blueberry. The flavonoids in raspberry consisted of quercetin aglycone and its glycosides. The mean total flavonoid content in blueberry [143.0 mg/100 g dry weight (DW)] was 1.5-times that in raspberry (95.4 mg/100 g DW). The most abundant flavonoid in blueberry was quercetin 3-O-galactoside (hyperoside, up to 76.1 mg/100 g DW) and that in raspberry was quercetin 3-O-glucuronide (miquelianin, up to 55.5 mg/100 g DW). Miquelianin was not detected in blueberry. CONCLUSION: Flavonol glycosides were the main flavonoids in blueberry and black raspberry cultivated in Korea. The composition and contents of flavonoids differed between blueberry and black raspberry, and may be affected by the cultivar and cultivation conditions.


Flavonoid;Rubus coreanus;UPLC-DAD-QTOF/MS;Vaccinium corymbosum


Supported by : Rural Development Administration


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