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Inhibitory Effect of Chlorine Dioxide Using Reactive Oxygen Species Against Heart Contraction of the Indianmeal Moth, Plodia interpunctella

이산화염소의 활성산소 발생에 따른 화랑곡나방 심장박동 억제 효과

  • Kim, Chul-young (Department of Plant Medicals, Andong National University) ;
  • Kwon, Hyeok (Department of Biosystems and Biotechnology, Korea University) ;
  • Kim, Wook (Department of Biosystems and Biotechnology, Korea University) ;
  • Kim, Yonggyun (Department of Plant Medicals, Andong National University)
  • 김철영 (안동대학교 식물의학과) ;
  • 권혁 (고려대학교 생명공학부) ;
  • 김욱 (고려대학교 생명공학부) ;
  • 김용균 (안동대학교 식물의학과)
  • Received : 2017.02.07
  • Accepted : 2017.04.15
  • Published : 2017.06.01

Abstract

Heart contraction is essential for insect hemolymph circulation, in which various physiological changes need control of the heart contraction. Thus, interruption of heartbeats intimidate insect survival. Chlorine dioxide induces release of reactive oxygen species (ROS), which has been regarded as the main insecticidal agent. This study analyzed the effect of chlorine dioxide on heartbeats of the Indianmeal moth, Plodia interpunctella. The larvae have the dorsal vessel on the medial dorsal region in a form of an extending tube from the first thorax to 10th abdominal segment. Dorsal vessel contraction was noticeable between 3rd and 10th abdominal segments, where five heart chambers were observed. Average heartbeat rate was 118.6 beats per min at $25^{\circ}C$. However, the heartbeats varied with ambient temperature. Injection of chlorine dioxide to hemocoel suppressed the heartbeats in a dose-dependent manner. The suppressive effect of chlorine dioxide also increased with exposure time to gas form of the chemical at 100 ppm. However, vitamin E (an antioxidant against ROS) treatment significantly rescued the larvae treated with chlorine dioxide in the heart contraction activity. These results suggest that chlorine dioxide negatively influences on the heart contraction of P. interpunctella via its production of ROS.

Acknowledgement

Supported by : 농림수산식품기술평가원

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