Stability of Ethanolic Extract from Fermented Cirsium setidens Nakai by Bioconversion during Different Storing Conditions

저장조건에 따른 생물전환 발효고려엉겅퀴 주정추출물의 안정성 조사

  • Received : 2017.03.08
  • Accepted : 2017.04.03
  • Published : 2017.04.30


This study investigated the stability of ethanolic extract from fermented Cirsium setidens Nakai (FCSN) in order to develop functional materials during different storage conditions. We evaluated pectolinarin and pectolinarigenin contents, total phenol content and antioxidant activity (DPPH radical scavenging activity and FRAP assay) of ethanolic extract from FCSN obtained by bioconverison at various temperatures (4, 25 and $50^{\circ}C$) and pHs (4.0, 7.0 and 10.0). Our results show that the pectolinarin, pectolinarigenin, and total phenol contents in ethanolic extract from FCSN were decreased during the storage periods. Moreover, the DPPH radical scavenging activity did not significantly change at $4^{\circ}C$ and $25^{\circ}C$. Pectolinarin and pectolinarigenin contents, total phenol content and DPPH radical scavenging activity of ethanolic extract from FCSN at acidic pH (pH 4.0) and neutral pH (pH 7.0) were higher than those at the alkaline pH range. These results indicate that the optimum storage condition of the ethanolic extract from FCSN was $4^{\circ}C$ and pH 4.0~7.0 range.


fermented;C. setidens Nakai;stability;pectolinarin;pectolinarigenin;antioxidant activity;total phenol content


  1. Biglari F, AlKarkhi AF, Easa AM. 2008. Antioxidant activity and phenolic content of various date palm (Phoenix dactylifera) fruits from Iran. Food Chem 107:1636-1641
  2. Bondet V, Brand-Williams W, Berset C. 1997. Kinetics and mechanisms of antioxidant activity using the DPPH free radical method. LWT-Food Sci Technol 30:609-615
  3. Chang SY, Song JH, Kwak YS, Han MJ. 2012. Quality characteristics of Gondre tofu by the level of Cirsium setidens powder and storage. Korean J Food Culture 27:737-742
  4. Duval B, Shetty K. 2001. The stimulation of phenolics and antioxidant activity in pea (Pisum sativum) elicited by genetically transformed anise root extract. J Food Biochem 25:361-377
  5. Ferenci P, Dragosics B, Dittrich H, Frank H, Benda L, Lochs H, Meryn S, Base W, Schneider B. 1989. Randomized controlled trial of silymarin treatment in patients with cirrhosis of the liver. J Hepatol 9:105-113
  6. Ham SS, Lee SY, Oh DH, Kim SH, Hong JK. 1997. Development of beverages drinks using mountain edible herbs. J Korean Soc Food Sci Nutr 26:92-97
  7. Hong JH, Kim HY, Kim JY, 2011. Factors affecting reactivity of various phenolic compounds with the Folin-Ciocalteu reagent. J Korean Soc Food Sci Nutr 40:205-213
  8. Hong MJ, Lee GD, Kim HK, Kwon JH. 1998. Changes in functional and sensory properties of chicory roots induced by roasting processes. Kor J Food Sci Technol 30:413-418
  9. Ishida H, Umino T, Tsuji K, Kosuge T. 1987. Studies on antihemorrhagic substances in herbs classified as hemostatics in Chinese medicine. VII. On the antihemorrhagic principle in Cirsium japonicum DC. Chem Pharm Bull (Tokyo) 35:861-864
  10. Im HE, Yoe HK, Chang SY, Han MJ. 2012. Quality characteristics of Gondregaedduck by the level of Cirsium setidens and storage. Korean J Food Culture 27:400-406
  11. Jeon HJ, Yu SN, Kim SH, Park SK, Choi HD, Kim KY, Lee SY, Chun SS, Ahn SC. 2014. Antiobesity effect of citrus peel extract fermented with Aspergillus oryzae. J Life Sci 4:827-836
  12. Kang IJ, Ham SS, Chung CK, Lee SY, Oh DH, Choi KP, Do JJ. 1997. Development of fermented soysauce using Cirsium setidens Nakai and comfrey. J Korean Soc Food Sci Nutr 26:1152-1158
  13. Kim BH, Kang JH, Lee SY, Cho HJ, Kim YJ, Kim YJ, Ahn SC. 2006. Biotransformation of ginseng to compound K by Aspergillus oryzae. J Life Sci 16:136-140
  14. Kim SH, Joo MH, Yoo SH. 2009. Structural identification and antioxidant properties of major anthocyanin extracted from Omija (Schizandra chinensis) fruit. J Food Sci 74:134-140
  15. Kim IB, Shin S, Lim BL, Seong GS, Lee YE. 2010. Bioconversion of soybean isoflavone by Lactobacillus plantarum and Bifidobacterium longum. Korean J Food Cookery Sci 17:214-219
  16. Kim YH, Bae DB, Lee JS, Park SO, Lee SJ, Cho OH, Lee OH. 2013. Determination of eleutherosides and ${\beta}$-glucan content from different parts and cultivating areas of A. senticosus and A. koreanum. J Korean Soc Food Sci Nutr 42:2082-2087
  17. Kim YH, Bae DB, Park SO, Lee SH, Cho OH, Lee OH. 2013. Method validation for the determination of eleutherosides and ${\beta}$-glucan in Acanthopanax koreanum. J Korean Soc Food Sci Nutr 42:1419-1425
  18. Lee SJ. 1966. Korean Folk Medicine. Seoul National University Press, Seoul, Korea. pp.145-146
  19. Lee OH, Kim JH, Kim YH, Lee YJ, Lee JS, Jo JH, Kim BG, Lim JK, Lee BY. 2014. Nutritional components and physiological activities of Cirsium setidens Nakai. J Korean Soc Food Sci Nutr 43:791-798
  20. Lee OH, Lee HB, Lee JS, Lee BY. 2005. Optimization of extraction condition and stability of olive leaf extract. Korean J Food Sci Technol 37:178-182
  21. Lee SH, Heo SI, Li L, Lee MJ, Wang MH. 2008. Antioxidant and hepatoprotective activities of Cirsium setidens Nakai against $CCl_4$-induced liver damage. Am J Chin Med 36:107-114
  22. Lee SH, Jin YS, Heo SI, Shim TH, Sa JH, Choi DS, Wang MH. 2006. Composition analysis and antioxidative activity from different organs of Cirsium setidens Nakai. Korean J Food Sci Technol 38:571-576
  23. Lee YJ, Lee JH, Kim YH, Kim JH, Yu SY, Kim DB, Lee JS, Cho ML. Cho JH, Kim BK, Lee BY, Lee OH. 2015. Assessment of the pectolinarin content and the radical scavenging-linked antiobesity activity of Cirsium setidens Nakai extracts. Food Sci Biotechnol 24:2235-2243
  24. Lee WB, Kwon HC, Cho OR, Lee KC, Choi Su, Baek NI, Lee KR. 2002. Phytochemical constituents of Cirsium setidens Nakai and their cytotoxicity against human cancer cell lines. Arch Pharm Res 25: 628-635
  25. Lim AK, Jung MJ, Kim DW, Hong JH, Jung HK, Kim KS, Kim YH, Kim DI. 2009. An extrapolation concentration decision effect antihyperlipidemic of aglycone isoflavone from biotransformation soybean on the fed high-fat diet rats. J Korean Soc Food Sci Nutr 38:1167-1173
  26. Noh H, Lee H, Kim E, Mu L, Rhee YK, Cho CW, Chung J. 2013. Inhibitory effect of a Cirsium setidens extract on hepatic fat accumulation in mice fed a high-fat diet via the induction of fatty acid ${\beta}$-oxidation. Biosci Biotechnol Biochem 77:1424-1429
  27. Oh JW, Lee JH, Cho ML, Shin GH, Kim JM, Choi SI, Jung TD, Kim YH, Lee SJ, Lee BJ, Park SJ, Lee OH. 2015. Development and validation of analytical method for pectolinarin and pectolinarigenin in fermented Cirsium setidens Nakai by bioconversion. J Korean Soc Food Sci Nutr 44:1504-1509
  28. Ozgen M, Reese RN, Tulio AZ, Scheerens JC, Miller AR. 2006. Modified 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) method to measure antioxidant capacity of selected small fruits and comparison to ferric reducing antioxidant power (FRAP) and 2,2'-diphenyl-1-picrylhydrazyl (DPPH) methods. J Agric Food Chem 54:1151-1157
  29. Park YH, Won EK, Son DJ. 2002. Effect of pH on the stability of green tea catechins. J Fd Hyg Safety 17:117-123
  30. Quan LH, Liang Z, Kim HB, Kim SH, Kim SY, Noh YD, Yang DC. 2008. Conversion of ginsenoside Rd to compound K by crude enzymes extracted from Lactobacillus brevis LH8. J Ginseng Res 32:226-231
  31. Suh JT, Ryu SY, Kim WB, Choi KS, Kim BH. 1996. Improvement of gemination rate by low temperature and development of effective shading cultivation of Cirsium setidens under rain shelter in highland. Korean J Plant Res 9:151-156
  32. Surh J, Kim JO, Kim MH, Lee JC, Lee BY, Kim MY, Yang HW, Yun S, Jeong HR. 2009. Nutritional properties, as food resources for menu development, of cubed snailfish, shaggy sea raven, and two kinds of wild vegetables that are staple products in Samcheok. Korean J Food Cookery Sci 25: 690-702
  33. Thao NT, Cuong TD, Hung TM, Lee JH, Na M, Son JK, Jung HJ, Fang Z, Woo MH, Choi JS, Min BS. 2011. Simultaneous determination of bioactive flavonoids in some selected Korean thistles by high-performance liquid chromatography. Arch Pharm Res 34:455-461
  34. Yoo MY, Kim SK, Yang JY, 2004. Characterization of an antioxidant from sporophyll of Undaris pinatifida. Kor J Micorbiol Biotechnol 32:307-311
  35. Yoo YM, Nam JH, Kim MY, Choi J, Park HJ. 2008. Pectolinarin and pectolinarigenin of Cirsium setidens prevent the hepatic injury in rats caused by D-galactosamine via an antioxidant mechanism. Biol Pharm Bull 31:760-764


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