Effects of different methionine sources on production and reproduction performance, egg quality and serum biochemical indices of broiler breeders

  • Xiao, Xue (Feed Science Institute, College of Animal Science, Zhejiang University) ;
  • Wang, Yongxia (College of Animal Science and Technology, Zhejiang Agricultural and Forestry University) ;
  • Liu, Weilong (Animal Husbandry and Veterinary Bureau) ;
  • Ju, Tingting (Feed Science Institute, College of Animal Science, Zhejiang University) ;
  • Zhan, Xiuan (Feed Science Institute, College of Animal Science, Zhejiang University)
  • Received : 2016.05.21
  • Accepted : 2016.12.07
  • Published : 2017.06.01


Objective: The study was conducted to evaluate the effects of different methionine (Met) sources on production performance, reproduction performance, egg quality and serum biochemical indices in broiler breeders. Methods: After receiving a basal diet (containing 0.25% Met) for a 2-wk pretreatment period, a total of 360 39-wk-old Lingnan yellow broiler breeders were randomly allocated to four treatments with six replicates each (15 birds per replicate). Breeders were fed with basal diets (control) or diets supplemented with DL-methionine (DLM), DL-2-hydroxy-4-methylthio butytric calcium (MHA-Ca) and coated DL-Met (CME) respectively. Results: The results showed that CME supplementation promoted laying rate and decreased feed-to-egg ratio (F/E) (p<0.05), DLM and MHA-Ca supplementation decreased F/E (p<0.05) when compared with control group. The rate of fertility, hatchability and birthrate were higher (p<0.05) in DLM, MHA-Ca, and CME groups than control group. Compared with control group, CME increased the eggshell thickness (p<0.05); MHA-Ca improved the eggshell thickness, shell ratio and eggshell strength (p<0.05). Results also showed that CME elevated the concentration of total protein in serum (p<0.05); MHA-Ca improved the concentration of calcium in serum (p<0.05). The concentration of serum uric acid in DLM, MHA-Ca, and CME groups was lower than that in control group (p<0.05). Besides, CME had higher concentrations of serum taurine, cysteine and cystanthionine (p<0.05) while MHA-Ca and DLM promoted the serum taurine concentration (p<0.05) compared with control group. Conclusion: Based on the results, it was concluded that Met supplementation could enhance the production and reproduction performance as well as the antioxidant status and egg quality of broiler breeders. In terms of improving the production performance, reproduction performance and antioxidant performance, CME was superior to DLM and MHA-Ca; but in regard to the enhancement of eggshell quality and serum Ca concentration, MHA-Ca was superior to DLM and CME.



Supported by : Zhejiang Province Key S&T


  1. Vazquez-Anon M, Gonzalez-Esquerra R, Saleh E. Evidence for 2-hydroxy-4 (methylthio) butanoic acid and DL-methionine having different dose responses in growing broilers. Poult Sci 2006;85:1409-20.
  2. Ai Q, Xie X. Effects of replacement of fish meal by soybean meal and supplementation of methionine in fish meal/soybean meal-based diets on growth performance of the southern catfish silurus meridionalis. J World Aquacult Soc 2005;36:498-507.
  3. Zhan XA, Li JX, Xu ZR. Effects of methionine and betaine supplementation on growth performance, carcase composition and metabolism of lipids in male broilers. Br Poult Sci 2006;47:576-80.
  4. Zhang LB, Guo YM. Effects of liquid DL-2-hydroxy-4-methylthio butanoic acid on growth performance and immune responses in broiler chickens. Poult Sci 2008;87:1370-6.
  5. Boebel KP, Baker DH. Efficacy of the calcium salt and free acid forms of methionine hydroxy analog for chicks. Poult Sci 1982;61:1167-75.
  6. Chi S, Tan B, Dong X, Yang Q, Liu H. Effects of supplemental coated or crystalline methionine in low-fishmeal diet on the growth performance and body composition of juvenile cobia Rachycentron canadum (Linnaeus). Chinese J Oceanol Limnol 2014;32:1297-306.
  7. Martin-Venegas R, Geraert PA, Ferrer R. Conversion of the methionine hydroxy analogue DL-2-hydroxy-(4-methylthio) butanoic acid to sulfur-containing amino acids in the chicken small intestine. Poult Sci 2006;85:1932-8.
  8. Sauer N, Emrich K, Piepho HP. Meta-analysis of the relative efficiency of methionine-hydroxy-analogue-free-acid compared with dl-methionine in broilers using nonlinear mixed models. Poult Sci 2008;87: 2023-31.
  9. Xie M, Hou SS, Huang W. Effect of excess methionine and methionine hydroxy analogue on growth performance and plasma homocysteine of growing Pekin ducks. Poult Sci 2007;86:1995-9.
  10. Van Weerden EJ, Schutte JB, Bertram HL. Comparison of DL-methionine, DL-methionine-Na, DL-methionine hydroxy analogue-Ca, and DL-methionine hydroxy analogue-free acid with layers. Poult Sci 1984;63:1793-99.
  11. Benefield BC, Patton RA, Stevenson MJ, Overton TR. Evaluation of rumen-protected methionine sources and period length on performance of lactating dairy cows within Latin squares. J Dairy Sci 2009; 92:4448-55.
  12. Liu Z, Bateman A, Bryant M, Abebe A, Roland D. Estimation of bioavailability of DL-methionine hydroxy analogue relative to DLmethionine in layers with exponential and slope-ratio models. Poult Sci 2004;83:1580-6.
  13. Bunchasak C, Silapasorn T. Effects of adding methionine in lowprotein diet on production performance, reproductive organs and chemical liver composition of laying hens under tropical conditions. Int J Poult Sci 2005;4:301-8.
  14. Meng GH, Song D, Li LB, et al. Dietary methionine requirement of Jing Brown layer hens from 9 to 17 weeks of age. J Anim Physiol Anim Nutr 2016; doi: 10.1111/jpn.12525.
  15. Ekmay RD, Mei SJ, Sakomura NK, Coon CN. The cysteine, total sulfur amino acid, tyrosine, phenylalanine+ tyrosine, and non-essential amino acid maintenance requirements of broiler breeders. Poult Sci 2016;95:1341-7.
  16. Willemsen H, Swennen Q, Everaert N. Effects of dietary supplementation of methionine and its hydroxy analog DL-2-hydroxy-4-methylthiobutanoic acid on growth performance, plasma hormone levels, and the redox status of broiler chickens exposed to high temperatures. Poult Sci 2011;90:2311-20.
  17. Dong Y, Guo XY, Shi ML. Maternal dietary supplementation with two sources of selenium affects the mortality and the antioxidative status of chick embryo at different developmental periods. Int J Agric Biol 2014;16:629-33.
  18. Yenice E, Mizrak C, Gultekin M, Atik Z, Tunca M. Effects of organic and inorganic forms of manganese, zinc, copper, and chromium on bioavailability of these minerals and calcium in late-phase laying hens. Biol Trace Elem Res 2015;167:300-7.
  19. Waheed S, Fatima I, Mannan A, Chaudhary MS, Qureshi IH. Trace element concentration in egg-yolk and egg-white of farm and domestic chicken eggs. Int J Environ Anal Chem 1985;21:333-44.
  20. Sun Z, Ma Q, Li Z, Ji C. Effect of partial substitution of dietary spraydried porcine plasma or fishmeal with soybean and shrimp protein hydrolysate on growth performance, nutrient digestibility and serum biochemical parameters of weanling piglets. Asian-Australas J Anim Sci 2009;22:1032-7.
  21. Dennis MD, Jefferson LS, Kimball SR. Role of p70S6K1-mediated phosphorylation of eIF4B and PDCD4 proteins in the regulation of protein synthesis. J Biol Chem 2012; 287:42890-9.
  22. Richards JD, Atwell CA, Vazquez-Anon M, Dibner JJ. Comparative in vitro and in vivo absorption of 2-hydroxy-4 (methylthio) butanoic acid and methionine in the broiler chicken. Poult Sci 2005;84:1397-405.
  23. Nakamori K, Koyama I, Nakamura T. Effectiveness of taurine in protecting biomembrane against oxidant. Chem Pharm Bull 1990;38: 3116-9.
  24. Riedijk MA, Stoll B, Chacko S. Methionine transmethylation and transsulfuration in the piglet gastrointestinal tract. Proc Natl Acad Sci USA 2007;104:3408-13.
  25. Thomas EL, Grisham MB, Melton DF. Evidence for a role of taurine in the in vitro oxidative toxicity of neutrophils toward erythrocytes. J Biol Chem 1985;260:3321-9.
  26. Puchala R, Pierzynowski SG, Sahlu T. Effects of methionine and hormones on amino acid concentration in the skin of Angora goats. Small Rumin Res 1998;29:93-102.
  27. Swennen Q, Geraert PA, Mercier Y. Effects of dietary protein content and 2-hydroxy-4-methylthiobutanoic acid or DL-methionine supplementation on performance and oxidative status of broiler chickens. Br J Nutr 2011;106:1845-54.
  28. China Feed Database. Table of feed composition and nutritive value in China: 1998 Edition. China Feed 1999;4:25-31.

Cited by

  1. Boone) vol.49, pp.9, 2018,